Fc receptor blocking was performed (clone 93; BioLegend) in FACS buffer (0.5% BSA, 2 mM EDTA, and 0.02% sodium azide in PBS) for 5 min at 4°C before surface staining (4°C for 30 min). The following anti-mouse antibodies from BioLegend were used for surface staining: APC/Cyanine7 anti-CD11b, PE/Cyanine7 anti-mouse CD19 Antibody, Brilliant Violet 711 anti-CD45, Alexa Fluor 647 anti-mouse CD206 (MMR) Antibody, Brilliant Violet 421 anti-mouse F4/80 Antibody, PE anti-mouse FcεRIα, Alexa Fluor 700 anti-mouse Ly-6C Antibody, Alexa Fluor 488 anti-mouse Ly-6G Antibody, Brilliant Violet 510 anti-mouse I-A/I-E Antibody, and PE/Cyanine7 anti-mouse Kit. The APC Rat Anti-Mouse CD19 was from BD Pharmingen. Live cells were distinguished by LIVE/DEAD Fixable Yellow Dead Cell Stain Kit (Thermo Fisher Scientific). Flow cytometry was performed on BD LSRFortessa X-20 flow cytometer. Flow cytometry data were analyzed with FlowJo software (Tree Star).
Brilliant violet 711 anti cd45
Manufactured by BioLegend
Brilliant Violet 711 anti-CD45 is a fluorochrome-conjugated antibody that binds to the CD45 protein, which is a common leukocyte antigen expressed on the surface of most hematopoietic cells. This product is designed for use in flow cytometry applications to identify and characterize cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Brilliant violet 421, by BioLegend (1 mentions)
Apc rat anti mouse cd19, by BD (1 mentions)
Brilliant violet 510, by BioLegend (1 mentions)
Apc cyanine7 anti cd11b, by BioLegend (1 mentions)
Anti mouse antibodies, by BioLegend (1 mentions)
Live dead fixable yellow dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Lsrfortessa x 20 flow cytometer, by BD (1 mentions)
1 step fix lyse solution, by Thermo Fisher Scientific (1 mentions)
Apc anti cd66b, by BioLegend (1 mentions)
Human fc receptor block, by BioLegend (1 mentions)
2 protocols using brilliant violet 711 anti cd45
1
Comprehensive Immune Cell Profiling
2
Multiparametric Flow Cytometry for Immune Cell Profiling
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100 µl whole blood was incubated in human Fc Receptor Block (Biolegend) followed by anti-human fluorophore-conjugated antibodies: PerCP/Cyanine5.5 anti-CD3), APC/Cyanine7 anti-CD11b, Brilliant Violet 650 anti-CD25, PE/Cyanine5 anti-CD62P/P-Selectin, Brilliant Violet 711 anti-CD45, APC anti-CD66b, Brilliant Violet 605 anti-CD56/NCAM, Alexa Fluor488 anti-CD14, Brilliant Violet 785 anti-CD8, PE anti-CD16 (all from Biolegend); Alexa Fluor594 anti-ACE-2 (R&D Systems), PerCP-eFluor710 anti-CD19 (eBioscience), and BV421 anti-HLA-DR, DP, DQ (BD Biosciences). Red blood cells were lysed and cells were fixed using 1-step Fix/Lyse Solution (eBioscience), rinsed with FACS buffer, and resuspended in FACS buffer (0.5% BSA, 0.005% EDTA, 1 × PBS). Samples were analyzed using the NovoCyte Quanteon flow cytometer, NovoSampler Q, and NovoExpress Software. An average of 1 × 105 events were collected from the “Live Cell” gate for analysis. Analysis was conducted using FlowJo software version 10. The complete gating strategy is provided in Supplemental Fig. 2 .
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