Skov3 (human ovarian carcinoma cells) were cultured in DMEM/F12 medium + FBS (10%) and antibiotics (100 U/mL penicillin together with 100 µg/mL streptomycin) in a 5% CO2 atmosphere at 37 °C in humidified incubator. Different concentrations of PPI (0, 100, 200, 400 μg/mL) were used to treat cells for 24 h, and the MTT assay was used to evaluate the cytotoxicity of PPI [40 (link)]. The S0033 detection kit (Beyotime, Haimen, Jiangsu, China) was used to measure the intracellular ROS levels in accordance with this method.
S0033 detection kit
Manufactured by Beyotime
Sourced in China
The S0033 detection kit is a laboratory equipment product that is designed to detect the presence of a specific analyte or compound in a sample. The kit provides the necessary reagents and materials to perform the detection process. The core function of the kit is to enable reliable and accurate identification of the target substance, without making any interpretations or extrapolations about its intended use or applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using s0033 detection kit
1
Cytotoxicity and ROS Evaluation of PPI in Skov3 Cells
2
Intracellular ROS and Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An S0033 detection kit (Beyotime) was used to measure intracellular reactive oxygen species (ROS) levels in accordance with the instructions. An MA0233 detection kit (Meilum one‐step terminal deoxyribonucleotide transferase‐mediated nick end labeling [TUNEL] apoptosis assay kit [FTIC], Melonepharma) was used to measure the TUNEL levels in accordance with the included instructions.
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