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Multimetal target

Manufactured by Nikon
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The Multimetal target is a laboratory equipment used for sputter coating applications. It consists of multiple metals arranged in a specific configuration, allowing for the deposition of complex metal alloy films onto substrates.

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Lab products found in correlation

Ct pro 3d, by Nikon (3 mentions) Parafilm m, by Amcor (2 mentions) Xt h 225 st microfocus ct scanner, by Nikon (2 mentions) Vgstudio max, by Volume Graphics (2 mentions) Xt h 225 st, by Nikon (1 mentions) Vgstudio max 3, by Volume Graphics (1 mentions)

3 protocols using multimetal target

1

Iodine-Enhanced Microfocus-CT Imaging of Embryos

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Embryos were fixed in 4% paraformaldehyde and potassium triiodide (Lugol's iodine/I2KI, to impart tissue contrast), with a total iodine content of 63.25 mg/mL (iodine mass of 2.49 × 10−4 mol/mL), in a 1:1 ratio for 8 h before imaging. Prior to scanning, the embryos were washed, wrapped in Parafilm M (Bemis, Oshkosh, WI, USA) and secured in 3% w/v Agar (Sigma-Aldrich, UK) within a low-density plastic cylinder to ensure mechanical stability during scan acquisition. Images were acquired using an XT H 225 ST microfocus-CT scanner with a multimetal target (Nikon Metrology, Tring, UK). Scans were reconstructed using modified Feldkamp filtered back projection algorithms with proprietary software (CTPro3D; Nikon Metrology) and post-processed using VG Studio MAX (Volume Graphics GmbH, Heidelberg, Germany). Soft tissues were analysed by Phong shading of direct volume renderings and plain projections and the vascular system by maximum intensity projections.
Peltzer N., Darding M., Montinaro A., Draber P., Draberova H., Kupka S., Rieser E., Fisher A., Hutchinson C., Taraborrelli L., Hartwig T., Lafont E., Haas T.L., Shimizu Y., Böiers C., Sarr A., Rickard J., Alvarez-Diaz S., Ashworth M.T., Beal A., Enver T., Bertin J., Kaiser W., Strasser A., Silke J., Bouillet P, & Walczak H. (2018). LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature, 557(7703), 112-117.
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2

Iodine-Enhanced Microfocus-CT Imaging of Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryos were fixed in 4% paraformaldehyde and potassium triiodide (Lugol's iodine/I2KI, to impart tissue contrast), with a total iodine content of 63.25 mg/mL (iodine mass of 2.49 × 10−4 mol/mL), in a 1:1 ratio for 8 h before imaging. Prior to scanning, the embryos were washed, wrapped in Parafilm M (Bemis, Oshkosh, WI, USA) and secured in 3% w/v Agar (Sigma-Aldrich, UK) within a low-density plastic cylinder to ensure mechanical stability during scan acquisition. Images were acquired using an XT H 225 ST microfocus-CT scanner with a multimetal target (Nikon Metrology, Tring, UK). Scans were reconstructed using modified Feldkamp filtered back projection algorithms with proprietary software (CTPro3D; Nikon Metrology) and post-processed using VG Studio MAX (Volume Graphics GmbH, Heidelberg, Germany). Soft tissues were analysed by Phong shading of direct volume renderings and plain projections and the vascular system by maximum intensity projections.
Peltzer N., Darding M., Montinaro A., Draber P., Draberova H., Kupka S., Rieser E., Fisher A., Hutchinson C., Taraborrelli L., Hartwig T., Lafont E., Haas T.L., Shimizu Y., Böiers C., Sarr A., Rickard J., Alvarez-Diaz S., Ashworth M.T., Beal A., Enver T., Bertin J., Kaiser W., Strasser A., Silke J., Bouillet P, & Walczak H. (2018). LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature, 557(7703), 112-117.
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3

Micro-CT imaging of fetal specimens

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Micro CT images of the specimens were acquired using an XT H 225 ST microfocus-CT scanner with a multi-metal target (Nikon Metrology, Tring, UK). X-ray energies and beam current were between 80 -110 kV and 87-180 µA respectively. Exposure times were from 250 ms to 354 ms, with the number of projections optimized for the size of the specimen (number of pixels covered within area of interest x 1.5) and one X-ray frame per projection. Where possible, each fetus was scanned three times (approximately 19 minutes each, total scan time approximately 57 minutes), to provide one overview whole body dataset at lower magnification followed by two higher-magnification scans of the brain, and thorax & abdomen. Projection images were reconstructed using modified Feldkamp filtered back-projection algorithms with proprietary software (CTPro3D; Nikon Metrology, UK) and post processed using VG Studio MAX 3.0 (Volume Graphics GmbH, Heidelberg, Germany). Isotropic voxel sizes varied according to specimen size and magnification achieved and ranged from 7.4 µm to 51.0 µm.
Hutchinson J.C., Kang X., Shelmerdine S.C., Segers V., Lombardi C.M., Cannie M.M., Sebire N.J., Jani J.C, & Arthurs O.J. (2018). Postmortem microfocus computed tomography for early gestation fetuses: a validation study against conventional autopsy. American journal of obstetrics and gynecology, 218(4).
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