Primary antibodies used for Western blotting and immunofluorescence staining were mouse anti-K7 (RCK-105; Progen, Heidelberg, Germany), rat anti-K8 and rat anti-K19 (Troma I and Troma III, respectively; Developmental Studies Hybridoma Bank, Iowa, IA, USA), rabbit anti-K8 (273) and rabbit anti-K18 (275; kind gifts from J.E. Eriksson), rabbit anti-K20 (It-Ks 20.10; Epitomics, Burlingame, CA, USA), rat anti-Hsc70 (Enzo Life sciences; Farmingdale, NY, USA), mouse anti-K8 pS74 (LJ4; kind gift from M.B. Omary), rabbit anti-Ki67 (Abcam, Cambridge, MA, USA), rat anti-HSF2 (Abcam) and rabbit anti-IκB-α (Santa Cruz Biotechnology; Dallas, TX, USA). Secondary antibodies used for Western blotting were HRP-conjugated anti-mouse (GE healthcare, Little Chalfont, UK), anti-rat (GE healthcare and Cell Signaling Technology, Danvers, MA, USA) and anti-rabbit (Cell Signaling Technology) IgG antibodies. Secondary antibodies used for immunofluorescence staining were Alexa 488/Alexa 546 anti-mouse, Alexa 488 anti-rat and Alexa 488 anti-rabbit antibodies (Invitrogen, Carlsbad, CA, USA). Nuclei were stained with DRAQ5 (Cell Signaling Technology).
Rat anti k8
Manufactured by Developmental Studies Hybridoma Bank
Sourced in Germany, United States
Rat anti-K8 is a monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is designed to specifically recognize and bind to the K8 (Keratin 8) protein. The primary function of this antibody is to serve as a research tool for the detection and analysis of K8 in various biological samples and applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti vimentin, by Cell Signaling Technology (2 mentions)
Rabbit anti oct4, by Abcam (2 mentions)
Mouse anti tra 1 60, by Thermo Fisher Scientific (2 mentions)
Rabbit anti lamin b1, by Abcam (2 mentions)
Mouse anti gigaxonin, by Santa Cruz Biotechnology (2 mentions)
Mouse anti keratin 8, by Thermo Fisher Scientific (2 mentions)
Mouse anti nestin, by Thermo Fisher Scientific (2 mentions)
Mouse anti tra 1 81, by Thermo Fisher Scientific (2 mentions)
Mouse anti vimentin, by Thermo Fisher Scientific (2 mentions)
Rabbit anti lamin a c, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti sox2, by Thermo Fisher Scientific (2 mentions)
Draq5, by Cell Signaling Technology (1 mentions)
Rabbit anti iκbα, by Santa Cruz Biotechnology (1 mentions)
Mouse anti k7 rck 105, by Progen Biotechnik (1 mentions)
Alexa 488 anti rat, by Thermo Fisher Scientific (1 mentions)
Rat anti hsc70, by Enzo Life Sciences (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
Mouse anti gfap, by Merck Group (1 mentions)
Rabbit anti gfap, by Agilent Technologies (1 mentions)
X ray film, by Fujifilm (1 mentions)
4 protocols using rat anti k8
1
Immunodetection of Cytoskeletal Proteins
2
Comprehensive Immunofluorescence and Western Blot Antibody Protocol
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The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma, clone GA5; IF 1:300), mouse anti-Gigaxonin (Santa Cruz Biotechnology, F3, WB 1:200), rabbit anti-Vimentin (Cell-Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific, V9, WB 1:1000), mouse anti-Keratin 8 (Thermo Fisher Scientific, TS1, IF 1:100), rat anti-K8 (Developmental Studies Hybridoma Bank, Troma I, WB 1:5000), rabbit anti-Lamin A/C (Santa Cruz Biotechnology), rabbit anti-Lamin B1 (Abcam ab16048, IF 1:10,000, WB 1:10,000), mouse anti-Nestin (Thermo Fisher Scientific, 10C2, IF 1:200), mouse anti-NF-M/H (clone RMdO-20; IF 1:100), mouse anti-Tra-1-60 (Thermo Fisher Scientific, 41-1000, IF 1:300), mouse anti-Tra-1-81 (Thermo Fisher Scientific, 41-1100, IF 1:300), rabbit anti-Oct4 (Abcam, ab19857, IF 1:40), and rabbit anti-Sox2 (Thermo Fisher Scientific, 48-1400, IF 1:125). The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat-, anti-mouse-, and rabbit-antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat-, anti-mouse-, and rabbit-antibodies (Sigma, WB 1:5000).
3
Islet Protein Analysis by Western Blot
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Isolated and hand‐picked islets from K8+/+ and K8−/− mice were homogenized with a 1‐mL syringe (BD, Franklin Lakes, NJ, USA) and 30‐G needle (Henke Sass Wolf, Tuttlingen, Germany), and samples were prepared for SDS‐PAGE and Western blotting as described.20 Primary antibodies used were as follows: rabbit anti‐insulin (Santa Cruz Biotechnologies), rat anti‐K8 (Troma I; Developmental Studies Hybridoma Bank), rabbit anti‐K18 (275, kind gift from Professor J.E. Eriksson), rat anti‐Hsc70 (Stressgen Bioreagents, Ann Arbor, MI, USA), rabbit anti‐GLUT2 (Polyclonal; Millipore) and rabbit anti‐MFN2 (Sigma‐Aldrich, St. Louis, MO, USA). Anti‐rabbit HRP (Promega Biosciences, San Luis Obispo, CA, USA) and anti‐rat HRP (GE Healthcare, Little Chalfont, UK) secondary antibodies were used. The signal on PVDF‐membranes was developed with ECL developing solution (GE Healthcare) and further exposed to X‐ray films (Fuji, Tokyo, Japan). The Western blot films were then analysed with IMAGE J software (NIH) for individual band quantification.
4
Comprehensive Antibody Panel for Cellular Characterization
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The following primary antibodies and concentrations were used in this study: rabbit anti-GFAP (DAKO, Agilent, clone Z0334; IF 1:500, WB 1:10,000), mouse anti-GFAP (Sigma, clone GA5; IF 1:300), mouse anti-pSer13-GFAP (gift from Dr. Masaki Inagaki, clone KT13, IF 1:20), mouse anti-Gigaxonin (Santa Cruz Biotechnology, F3, WB 1:200), rabbit anti-Vimentin (Cell Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific, V9, WB 1:1000), mouse anti-Keratin 8 (Thermo Fisher Scientific, TS1, IF 1:100), rat anti-K8 (Developmental Studies Hybridoma Bank, Troma I, WB 1:5000), rabbit anti-Lamin A/C (Santa Cruz Biotechnology), rabbit anti-Lamin B1 (Abcam ab16048, IF 1:10,000, WB 1:10,000), mouse anti-Nestin (Thermo Fisher Scientific, 10C2, IF 1:200), mouse anti-Tra-1–60 (Thermo Fisher Scientific, 41–1000, IF 1:300), mouse anti-Tra-1–81 (Thermo Fisher Scientific, 41–1100, IF 1:300), rabbit anti-Oct4 (Abcam, ab19857, IF 1:40), and rabbit anti-Sox2 (Thermo Fisher Scientific, 48–1400, IF 1:125). The following secondary antibodies and concentrations were used: Alexa 488- and Alexa 594-conjugated goat anti mouse and rabbit antibodies (Thermo Fisher Scientific, IF 1:500), and peroxidase-conjugated goat anti-mouse and rabbit antibodies (Sigma, WB 1:5000).
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