Succinylated wheat germ agglutinin

Manufactured by Vector Laboratories

Sourced in United States

Succinylated wheat germ agglutinin is a lectin derived from wheat germ. It has the ability to bind to N-acetylglucosamine and sialic acid residues on cell surfaces.

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Lab products found in correlation

Sambucus nigra, by Vector Laboratories (1 mentions) Near ir fluorescent reactive dye, by Thermo Fisher Scientific (1 mentions) Lens culinaris agglutinin, by Vector Laboratories (1 mentions) Griffonia simplicifolia, by Vector Laboratories (1 mentions) Pisum sativum agglutinin, by Vector Laboratories (1 mentions) Concanavalin a type 4, by Merck Group (1 mentions) Phaseolus vulgaris erythroagglutinin, by Vector Laboratories (1 mentions) Phaseolus vulgaris leucoagglutinin, by Vector Laboratories (1 mentions) Streptavidin pe, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions)

2 protocols using succinylated wheat germ agglutinin

Quantifying Bacterial Cell Wall Protein Binding

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To examine the binding of fluorescent proteins to sacculi, 0.5 mL of sacculi resuspended in PBS (OD₆₀₀ of 3.4) were incubated with 48 μg mL⁻¹ AtlA^BSP-GFP, or 67 μg mL⁻¹ AtlA^FL-GFP, or 45 μg mL⁻¹ AtlA^C-GFP, or 46 μg mL⁻¹ GFP-eMapZ, or 28 μg mL⁻¹ GFP, or 10 μg mL⁻¹ succinylated wheat germ agglutinin (Vectorlabs) for 1 hour with agitation. Sample aliquots were assayed to determine the total fluorescence. Then samples were centrifuged (16,000 g, 3 min), followed by two washes with PBS. The pellet was resuspended in 0.5 mL of PBS, and sample aliquots were assayed to determine the pellet fluorescence. Data are presented as a percentage of fluorescence of the pellet normalized to the total fluorescence of the sample.
To examine the binding of AtlA^BSP-GFP to cell wall material, 0.5 mg of lyophilized cell wall was incubated with 48 μg mL⁻¹ AtlA^BSP-GFP in 0.5 mL of PBS. The experiment was conducted as described above.

Zamakhaeva S., Chaton C.T., Rush J.S., Castro S.A., Kenner C.W., Yarawsky A.E., Herr A.B., van Sorge N.M., Dorfmueller H.C., Frolenkov G.I., Korotkov K.V, & Korotkova N. (2021). Modification of cell wall polysaccharide guides cell division in Streptococcus mutans. Nature chemical biology, 17(8), 878-887.

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Glycan Profiling of Confluent Cells

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Confluent cells were detached from cell culture dishes by gentle resuspension in PBS and were transferred to V-bottom 96-well plates for staining (1–2 × 10⁶ cells/well). Prior to this, control cells were treated with 4 µg/ml kifunensine (Bio-Techne, Minneapolis, MN, USA) overnight. Cells were washed with staining buffer (PBS supplemented with 2% (v/v) fetal calf serum, 2 mM EDTA) and were then stained successively with a near-IR fluorescent reactive dye (1:500, Thermo Fisher Scientific), biotin-conjugated lectins and streptavidin-PE (1:1000, Thermo Fisher Scientific) diluted in FACS buffer. The following biotinylated lectins were used: Sambucus nigra (5 µg/ml), Griffonia simplicifolia (0.5 µg/ml), Pisum sativum agglutinin (1 µg/ml), Lens culinaris agglutinin (0.5 µg/ml), Phaseolus vulgaris erythroagglutinin (1 µg/ml), Phaseolus vulgaris leucoagglutinin (1 µg/ml), and succinylated wheat germ agglutinin (2 µg/ml) (all from Vector laboratories, San Francisco, CA, USA) as well as Concanavalin A Type IV (1 µg/ml) and Tritium vulgaris (0.5 µg/ml) (from Sigma–Aldrich). Stained cells were fixed with 2% formaldehyde (FA) in PBS (Polysciences) and were analysed using a FACSCanto II and the FlowJo 10.6.2 software package (Becton Dickinson).

Hobohm L., Koudelka T., Bahr F.H., Truberg J., Kapell S., Schacht S.S., Meisinger D., Mengel M., Jochimsen A., Hofmann A., Heintz L., Tholey A, & Voss M. (2022). N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion. Cellular and Molecular Life Sciences, 79(3), 185.

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