Hiprep sephacryl s 200 hr column

Manufactured by GE Healthcare

The HiPrep Sephacryl S-200 HR column is a size-exclusion chromatography column designed for the separation and purification of biomolecules. It is composed of a Sephacryl matrix, which provides a porous structure for size-based separation. The column is suitable for fractionation of proteins, peptides, and other macromolecules within a specific molecular weight range.

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Lab products found in correlation

Kta fast protein liquid chromatography system, by GE Healthcare (1 mentions) Vivaspin spin columns, by GE Healthcare (1 mentions)

Close spelling variants of this product

HiPrep 16/60 Sephacryl S-200 HR column HiPrep 26/60 Sephacryl S-200 HR size-exclusion column HiPrep 16/60 Sephacryl S-200 HR gel filtration column HiPrep 26/60 Sephacryl S-200 HR column HiPrep Sephacryl S-200 HR Sephacryl S-200 HR Sephacryl S-200

2 protocols using hiprep sephacryl s 200 hr column

Purification of Recombinant PHGDH Protein

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Either recombinant His-PHGDH or recombinant untagged PHGDH was analyzed using a GE HiPrep Sephacryl S-200 HR column. The column was run at 0.5 ml/min for one-column volume in storage buffer. Fractions were collected from just before the void volume to the elution volume of a 17-kDa standard. Fractions were then subjected to SDS-PAGE, and the gels were stained with colloidal Coomassie. After destaining, the gel was imaged and quantitated as described above.

Mattaini K.R., Brignole E.J., Kini M., Davidson S.M., Fiske B.P., Drennan C.L, & Vander Heiden M.G. (2015). An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation. Cancer & Metabolism, 3, 5.

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Purification of MSA/ABD-iTEP-pOVA Complex

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MSA was incubated with ABD-iTEP-pOVA overnight at 4 °C. Then MSA/ABD-iTEP-pOVA was purified by ÄKTA fast protein liquid chromatography system (GE Healthcare) equipped with a HiPrep Sephacryl S-200 HR column (diameter: 26 mm, length: 600 mm, particle diameter: 50 µm, GE Healthcare). Samples were eluted with PBS at a flow rate of 1 mL/min. The fractions containing the MSA/ABD-iTEP-pOVA complex were combined and concentrated by Vivaspin spin columns (Molecular mass cut-off: 10,000 kDa, GE Healthcare).

Wang P., Zhao P., Dong S., Xu T., He X, & Chen M. (2018). An Albumin-binding Polypeptide Both Targets Cytotoxic T Lymphocyte Vaccines to Lymph Nodes and Boosts Vaccine Presentation by Dendritic Cells. Theranostics, 8(1), 223-236.

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