Nupage novex bis tris midi gel 15 or 26 wells

Total protein concentrations were determined using the BCA kit (Pierce). Equal amounts of total protein extract (30 µg) were electrophoretically separated using SDS-PAGE in 4-12% Bis-Tris gels (NuPAGE ® Novex Bis-tris midi gel 15 or 26 wells, Life Technologies, Carlsbad, USA) and transferred to nitrocellulose membranes. Blocked membranes (5% non-fat dry milk in TBS-0.1% Tween-20) were incubated with primary antibodies overnight at 4 °C and washed three times with TBS-0.1% Tween-20 (T-BST) for 10 min. Membranes were then labeled with secondary IgG-HRP antibodies raised against each corresponding primary antibody. After three washes with T-BST, the membranes were incubated with ECL chemiluminescent reagent (Clarity Western ECL substrate; GE Healthcare, Little Chalfont, UK) according to the instructions of the supplier. Peroxydase activity was detected with a Fusion TX7 camera system (Fisher Scientific). Quantification was made by densitometric analysis with the Quantity One 1D image analysis software (version 4.4; Biorad, Hercules, CA, USA). The optical densities were normalized with respect to a "standard protein" (GAPDH/actin). For each sample, a partition ratio was calculated and normalized with respect to the sample with the highest value defined as 1.