The prefrontal cortical tissues were collected and isolated. The levels of 8-OH-dG were measured using commercially available assay kits (8-hydroxy 2 deoxyguanosine ELISA Kit, ab201734, Cayman, Ann Arbor, MI, USA).
8 hydroxy 2 deoxyguanosine elisa kit
Manufactured by Cayman Chemical
Sourced in United States
The 8-hydroxy 2 deoxyguanosine ELISA Kit is a laboratory tool used to quantify the levels of 8-hydroxy-2'-deoxyguanosine, a biomarker for oxidative stress, in biological samples. The kit employs a competitive enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of the target analyte.
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3 protocols using 8 hydroxy 2 deoxyguanosine elisa kit
1
Prefrontal Cortex 8-OH-dG Quantification
2
Prefrontal Cortex 8-OH-dG Levels
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The prefrontal cortical tissues were collected and isolated. The levels of 8-OH-dG were measured using commercially available assay kits (8-hydroxy 2 deoxyguanosine ELISA Kit, Cayman, Ann Arbor, MI, USA).
3
Quantifying Reactive Oxygen Species and DNA Damage
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Cells were cultivated in 96 well black plates according to the manufacturer's instructions. This test was performed 48 h after the exposure (40,000 cells/well) using the DHE (Dihydroethidium) Assay Kit-Reactive Ox Species (ab236206, Abcam China, Shanghai, China), according to manufacturer's protocol. It allows the quantification of reactive oxygen Species (Superoxide radical and hydrogen peroxide) in living cells, and uses dihydroethidium DHE as a fluorescent probe for the detection of ROS.
Assessment of oxidative DNA damage -quantification of 8-OHdG 8-hydroxy-2'-deoxyguanosine (8-OHdG) is formed as a result of oxidative damage to DNA by reactive oxygen and nitrogen species. The concentration of 8-OHdG was measured using 8-hydroxy-2'-deoxyguanosine ELISA Kit, (ab201734, Cayman, Ann Arbor, MI, USA), according to manufacturer's protocol. The assay uses a plate coated with 8-hydroxy-2'deoxyguanosine, and an antibody, bound to the enzyme horseradish peroxidase (HRP) for detection (Ndong Ba et al., 2019) .
Assessment of oxidative DNA damage -quantification of 8-OHdG 8-hydroxy-2'-deoxyguanosine (8-OHdG) is formed as a result of oxidative damage to DNA by reactive oxygen and nitrogen species. The concentration of 8-OHdG was measured using 8-hydroxy-2'-deoxyguanosine ELISA Kit, (ab201734, Cayman, Ann Arbor, MI, USA), according to manufacturer's protocol. The assay uses a plate coated with 8-hydroxy-2'deoxyguanosine, and an antibody, bound to the enzyme horseradish peroxidase (HRP) for detection (Ndong Ba et al., 2019) .
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