Gelred nucleic acid gel stain 10 000x

The DNA was extracted from fresh stool using the Norgen Stool DNA Isolation Kit (Norgen, Biotek Corporation) and stored at -20°C. PCR was performed with the 2X PCR Taq Plus MasterMix (Applied Biological Materials Inc ABM) using Blastocystis-specific primers F:5′-GAAGGACTCTCTGACGATGA-3'/R:5′-GTCCAAATGAAAGGCAGC-3' (351 bp) for subtype 1 (4), F:5′-CATGAGTAAAGTCCCGTWGGGA-3'/R:5′-CCCTTTTACAGTTCATTCGCCTA-3' (1500 bp) for subtype 2 (27) (link), and F:5′-TAGGATTTGGTGTTTGGAGA-3'/R:5′-TTAGAAGTGAAGGAGATGGAAG-3' (526 bp)
for subtype 3 (4) with the PCR setting parameters described in figure 1.
Electrophoresis was performed by adding 8 µl of the PCR products to a 1.7% agarose gel and staining with GelRed® Nucleic Acid Gel Stain, 10,000X in Water (Biotium Inc.) for 30 min at 100 V.