Elga purelab flex system

TNBS (MB5547), 5-ASA (MB7539), and DCFH-DA (MA0219) were purchased from Dalian Meilun Biotechnology Company, China. 1-phenyl 2-thiourea (PTU) (P7629) and Ethyl 3-aminobenzoate methanesulfonate (Tricaine, E10521) were purchased from Sigma-Aldrich company of USA. Puerarin, 3ʹ-methoxyPuerarin, daidzin, oroxindin, glycyrrhizic acid and esculin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Palmatine chloride, chlorogenic acid, oroxyloside, fraxin and phellodendrine were purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China). Berberine chloride was obtained from Dalian Meilun BiotechnologyCo., Ltd (Dalian, China). Baicalin was obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). The purities of all compounds were no less than 98%. HPLC-grade acetonitrile, methanol and formic acid were from Merk (Darmstadt, Germany). Deionized water was prepared with an Elga PURELAB flex system (ELGA LabWater, UK).

Poly (ethylene oxide)-b-poly (caprolactone) (PEO-PCL) 5k-4k, poly (ethylene oxide)-b-poly (styrene) (PEO-PS) 2,3k-3,1k were purchased from Polymer Source (Polymer Source, Inc., Dorval, Canada). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N- (lissamine rhodamine B sulphonyl) (ammonium salt) (Lys- Rhod-PE) come from Avanti Polar Lipids (Avanti Polar Lipids, Inc., USA). (N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) was bought from Thermo Fisher (NBD-PE, N360, Waltham, MA, US). Pheophorbide-a was purchased from Wako (Japan). Chloroform, methanol, acetone, phosphate buffer (PBS, P4417) and carboxyfluorescein (CBF, 21877) were purchased from Sigma Aldrich (Merck, Darmstadt, Germany). The water used to prepare all PBS solutions was ultrapure water at 18 MΩ resistivity obtained with an ELGA Purelab Flex system (ELGA LabWater, High Wycombe, UK). Anthracene-9,10-dipropionic acid disodium salt (ADPA) was purchased from Santa Cruz Biotechnology (Dallas, TX, US).