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Colorimetric elisa kit

Manufactured by Abcam

The Colorimetric ELISA kit is a laboratory equipment designed for quantitative measurement of target analytes in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique, where the presence of the target analyte is detected through a color change reaction. The kit provides a standardized and reliable method for researchers to measure the concentration of specific proteins, hormones, or other biomolecules in their samples.

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2 protocols using colorimetric elisa kit

1

Quantifying mCherry Protein Levels

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mCherry protein levels were quantified with a commercially available colorimetric ELISA kit (Abcam). Briefly, a tagged capture antibody and HRP-labeled detection antibody were mixed in solution with standards, controls, or samples generating a capture antibody/mCherry/detection antibody complex which is then immobilized to the wells of a 96-well plate coated with an anti-tag antibody. 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate was added, the reaction was stopped, and absorbance was measured at 450 nm.
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2

Quantifying Inflammatory Cytokines

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The levels of IL-1β and IL-18 in the cells or tissues were determined using a colorimetric ELISA kit (Abcam) according to the manufacturer’s instructions.
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