The morphology of the cells was imaged under a conventional light microscopy (Nikon, Japan). After recording images, all tangible cells (attached/viable cells and floating cells) were collected in the culture system, and the number of cells was counted in a hemocytometer chamber under a microscope.
Conventional light microscopy
Manufactured by Nikon
Sourced in Japan, United States
A conventional light microscope is a standard optical instrument used to magnify and observe small objects. It utilizes visible light and a series of lenses to increase the apparent size of the specimen, allowing for detailed examination. The core function of a conventional light microscope is to provide enhanced visual access to microscopic samples.
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Lab products found in correlation
2 protocols using conventional light microscopy
1
Cell Morphology and Quantification
2
Ovarian Protein Localization Using IHC
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For immunohistochemical localization of proteins, the avidin-biotin-peroxidase complex was used on ovarian sections as previously described (Pascuali et al. 2018) . The utilized primary antibodies and their concentration are detailed in Table 1, lectin BS-1 was diluted 1:75 in PBS. Negative controls were obtained in the absence of the primary antibody.
Stained sections were analyzed and digitally photographed at 40×, 100× or 400× magnification by conventional light microscopy as needed (Nikon, Melville, NY, USA).
Stained sections were analyzed and digitally photographed at 40×, 100× or 400× magnification by conventional light microscopy as needed (Nikon, Melville, NY, USA).
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