Trichloroacetic Acid (TCA) pro analysis grade was purchased from Biopack (Buenos Aires, Argentina). Dimethyl Sulfoxide (DMSO) pro analysis grade was obtained from Anedra (Buenos Aires, Argentina). Chromatographic grade demineralized water (<0.2 μsiemens) was obtained in our laboratory with ionic interchange resins. Acetonitrile (AcN) and Methanol (Me) (J.T. Baker, USA) HPLCgrade were used. Sulfadiazine (Stanton L_1205050015269/0088) was obtained from the hospital pharmacy and pyrimethamine was obtained from sigma Aldrich.
A Table Top centrifuge (MRC, Scientific Instruments, Argentina) and a rotary evaporator (Heidolph Laborota 4010) equipped with a ROTAVAP valve control equipment were used for the pretreatment procedures. A certified 0.1 mg analytical (Ohaus-Pionner, USA) was used in weighing operations. All micropipettes were calibrated before use. All HPLC solvents were degassed with a vacuum pump (Pascal, Buenos Aires, Argentina). An ultrasonic homogenizer (FAETA, Argentina) was also used on extraction procedures.
A Table Top centrifuge (MRC, Scientific Instruments, Argentina) and a rotary evaporator (Heidolph Laborota 4010) equipped with a ROTAVAP valve control equipment were used for the pretreatment procedures. A certified 0.1 mg analytical (Ohaus-Pionner, USA) was used in weighing operations. All micropipettes were calibrated before use. All HPLC solvents were degassed with a vacuum pump (Pascal, Buenos Aires, Argentina). An ultrasonic homogenizer (FAETA, Argentina) was also used on extraction procedures.