Human saliva α amylase

Manufactured by Merck Group

Sourced in Germany, United States

Human saliva α-amylase is an enzyme found in human saliva that functions to break down starch into smaller carbohydrates. It is a key component in the initial stages of digestion.

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Lab products found in correlation

2 protocols using human saliva α amylase

Bioactive Compound Absorption Assay

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Caffeic acid, ferulic acid, sinapic acid, 5-O-caffeoylquinic acid (5-CQA), caffeine, human saliva α-amylase (300-1500 U/mg protein), pepsin (674 U/mg), pancreatin (4 x UPS) and bile salts were purchased from Sigma-Aldrich (Steinheim, Germany). 4,5-Dicaffeoylquinic acid (4,5-DiCQA) was obtained from Phytolab (Vestenbergsgreuth, Germany) and dihydroCaffeic acid and dihydroferulic acid from Alfa Aeser (Heysham, UK). Culture flasks, Transwell fitted with polycarbonate membranes were acquired from Corning® Costar® Transwell (Sigma Aldrich, St. Louis, US). Dulbecco's modified Eagle's medium (DMEM) was purchased from Gibco Invitrogen (Paisley, UK). The Caco-2 cell line PD7 clone was kindly provided by Dr. Edith Brot-Laroche (Paris, France). All chemicals and reagents used were of analytical grade from Panreac (Barcelona, Spain), Fisher Scientific (Loughborough, UK), and Sigma Aldrich (Steinheim, Germany).

Monente C., Ludwig I.A., Stalmach A., de Peña M.P., Cid C, & Crozier A. (2015). In vitro studies on the stability in the proximal gastrointestinal tract and bioaccessibility in Caco-2 cells of chlorogenic acids from spent coffee grounds. International journal of food sciences and nutrition, 66(6).

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Evaluation of Bioactive Compounds

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Human saliva α-amylase (14 µkat /mg proteins), pepsin from porcine gastric (11 µkat /mg), pancreatin from porcin pancreas (4xUPS, 0.12 µkat of trypsin/mg), α-amylase from Bacillus licheniformis, α-glucosidase and bile salts were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Solvents including acetone, methanol, acetic acid and acetonitrile were analytical grade and purchased from Sigma-Aldrich. Folin-ciocalteau reagent, sodium carbonate, 2,2-dipheny-1-picryhydrazyl radical (DPPH), fluorescein, trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), 2,2-azobis (2-methylpropionamidine) dihydrochloride (AAPH), potassium persulfate, DNS (3,5-dinitrosalicylic acid), p-nitrophenyl-α-D-glucopyranoside and sodium hydroxide (NaOH) were obtained from Sigma-Aldrich (Oakville, ON, Canada). The phenolic acid standards (over ≥ 980 g/Kg pure); gallic, protocatechic, chlorogenic, caffeic, vanillic, syringic, p-coumaric, ferulic, o-coumaric, trans-cinnamic, the flavanoid standards; (+)-catechin, rutin, isoquercitrin, myricetin, apigenin and kaempherol were also purchased from Sigma-Aldrich (St. Louis, Missouri, USA). De Man, Rogosa and Sharp (MRS), M17 and lactose used to growth the bacterial strains were purchased from (Sigma Aldrich, Canada).

Chait Y.A., Gunenc A., Bendali F., & Hosseinian F. (2021). Functional fermented carob milk: Probiotic variability and polyphenolic profile. Journal of Food Bioactives, 14.

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