Software of automated hematology analyzer for animal xn 5 series

Blood EDTA samples were used for complete blood cell count (CBC) with the hematology analyzer XN-1000 V (Sysmex Europe GmbH, Norderstedt, Germany), equipped with veterinary software (Software of Automated hematology Analyzer for Animal XN-V series (Sysmex). All samples were analyzed within 8 h after blood collection. Blood plain tubes were centrifuged 3000× g for 10 min, and serum samples were collected for biochemical analysis: routine biochemical profile analysis was performed using an automated clinical chemistry analyzer (Cobas C501; Roche Diagnostics International AG, Rotkreuz, Switzerland); folate and cobalamin (B12) immunoassay analysis was performed with an automated Cobas e601 analyzer (Roche Diagnostics). Serum canine C reactive protein (cCRP) concentration (mg/L) was determined via a commercially available turbidimetric immunoassay kit (Turbovet canine CRP, Acuvet Biotech, Zaragoza, Spain) applied to a Cobas C501 analyzer, following the manufacturer’s instructions. Fecal calprotectin was determined via a species-specific ELISA kit (Canine Calprotectin CP, MyBioSource, San Diego, CA, USA), following the manufacturer’s instructions. Briefly, 10 mg of samples were homogenized in 100 μL of PBS and centrifuged for 20 min at 1500× g; the supernatant was carefully collected and stored at −80 °C until analysis. Fecal samples were then thawed and measured in a unique batch.