Fixed femora were embedded in methylmethacrylate [19] . Osteoclasts were identified on longitudinal sections (5 μm) of the distal femurs using tartrate-resistant acid phosphatase (TRAP) staining [20] . TRAP staining was photographed using a Aperio ScanScope CS slide scanner (Leica Biosystems) and analysed using Image J-based software [21] .
Aperio scanscope cs slide scanner
Manufactured by Leica
Sourced in United States, United Kingdom
The Aperio ScanScope CS Slide Scanner is a digital slide scanner designed for high-throughput scanning of microscope slides. The device captures high-resolution images of the entire slide, allowing for detailed examination and analysis of the samples.
Automatically generated - may contain errors
Lab products found in correlation
Aperio imagescope, by Leica (3 mentions)
Imagescope software, by Leica (2 mentions)
Imagescope, by Leica (2 mentions)
Novolink max polymer detection system, by Leica (2 mentions)
β catenin d10a8, by Cell Signaling Technology (2 mentions)
Pser473 akt d9e, by Cell Signaling Technology (2 mentions)
Aperio imagescope software version 12, by Leica (1 mentions)
Aperio imagescope v12, by Leica (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Kaiser s glycerol gelatine, by Merck Group (1 mentions)
Oil red o solution, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Ep1454y, by Abcam (1 mentions)
Nuclear v9 algorithm, by Leica (1 mentions)
Birc5, by Santa Cruz Biotechnology (1 mentions)
Aurka, by Abcam (1 mentions)
Egfr pharmdx kit, by Agilent Technologies (1 mentions)
Grp94, by Abcam (1 mentions)
Benchmark xt platform, by Roche (1 mentions)
Fjk 16, by Thermo Fisher Scientific (1 mentions)
22 protocols using aperio scanscope cs slide scanner
1
Osteoclast Quantification in Femurs
2
Lung Fixation and Histological Analysis
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Following induction of isoflurane anesthesia, a midline abdominal incision was made, and the abdominal aorta was transected to exsanguinate and euthanize the animal. A midline incision was continued through the thoracic cavity and neck to allow injection of 5 ml of normal saline into the right ventricle of the heart to flush the pulmonary vasculature of residual blood. A luer lock–hubbed 20-gauge × ½-inch cannula was inserted into the trachea and secured in place with a suture. The lungs were fixed by insufflation with 10% neutral buffered formalin at 20 cm H2O for 24 hours at room temperature. The lung lobes were removed and embedded in paraffin, and 5-μm tissue slices were prepared and stained with hematoxylin and eosin by standard histopathology techniques (22 (link)). Histology images were acquired with an Aperio ScanScope CS slide scanner (Leica Biosystems) using a 40× objective lens and subsequently analyzed using Aperio ImageScope software version 12.3 (Leica Biosystems).
3
Histological Analysis of Hind Paw Sections
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H&E sections were assessed using an Axioskope mot plus microscope (Zeiss, Toronto ON). Scans of whole hind paw sections (maximum resolution 20x) were acquired using an Aperio ScanScope CS slide scanner (Leica Biosystems, Concord, ON). High resolution images (Figures 5 e–h; 6 e–h) were generated by zooming in on the whole paw scans using Aperio ImageScope v12.2.2.5015 (Leica Biosystems, Buffalo Grove, IL). Images of cartilage and CD163+ staining (Figure 7 e–m) were captured using a QIClick CCD Camera (QImaging, Surrey, BC) and processed using Northern Eclipse version 8 (Empix, Mississauga, ON).
4
Quantitative Analysis of Pulmonary Fibrosis
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The left lung was fixed overnight in 10% formalin (05-01004F BioOptica), dehydrated in a graded ethanol series, embedded in paraffin and cut into 4-µm sections. Pulmonary fibrosis was analyzed using digital microscopy. For the analysis of the fibrosis area, sections were stained with Masson’s trichrome, digitalized by AperioScanScope CS Slide Scanner (Leica Biosystem, New Castle Ltd, UK) at 40x magnification and submitted to ImageScope software (Leica).
5
Oil Red O Staining of Lipid Droplets
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HSC were cultured on coverslips and fixed with 4% paraformaldehyde (Merck Millipore). Coverslips were rinsed with 60% isopropanol and incubated with Oil Red O solution (Sigma-Aldrich) for 10 min. Afterward, cells were rinsed with 60% isopropanol and incubated with hematoxylin (Sigma-Aldrich) and tap water for 1 and 5 min, respectively. Slides were mounted with Kaiser's glycerol gelatine (Merck Millipore), scanned with Aperio Scanscope CS slide scanner (Leica Biosystems), and analyzed by Imagescope (Leica Biosystems, http://www.le-icabiosystems.com/pathology-imaging/aperio-epathology/ integrate/Imagescope/).
6
Quantifying Microvascular Density using CD34 and CD105
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The MVD of blood vessels stained with CD34 and CD105 was obtained by digital analysis. The slides were scanned into high-resolution images using the Aperio Scanscope CS Slide Scanner (Aperio Technologies Inc, Vista, CA, USA). All digital images obtained in .svs format were visualized with the ImageScope software (Aperio
7
Quantifying Immune Cell Populations in Ovarian Cancer
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Cell density of selected immune populations was analyzed using digital microscopy. The absolute cell count was quantified automatically using a custom-programmed script in Cognition Network Language based on the Definiens Cognition Network Technology platform (Definiens AG, Munich, Germany). Briefly, CD3, CD163, and BDCA2 stained slides were digitalized using an Aperio ScanScope CS Slide Scanner (Aperio Technologies, (Leica Biosystem, New Castle Ltd, UK) at 40× magnification and analyzed using Tissue Studio 2.0 (Definiens AG). The quantitative scoring algorithm was customized using commercially available templates (Supplementary Figure S1
8
Quantifying Kidney Fibrosis in IRI
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Under terminal general anesthesia, the left kidney in warm IRI experiments was harvested at 30 d after the ischemia experiment, fixed in 10% neutral-buffered formalin, and paraffin embedded. Histologic sections (4 μm) were stained with Sirius Red for quantification of fibrosis. Sirius Red-stained sections were scanned using the Aperio ScanScope CS slide scanner (Aperio Technologies, Leica Biosystems), and digitized images were analyzed with Aperio ImageScope software using an algorithm optimized for the detection of interstitial fibrosis (red staining of collagen fibers) as a percentage of the total volume of tissue on the slide. 19 Subcapsular areas were excluded so as to limit the analysis to parenchymal changes.
9
Histopathological Evaluation of Mouse Colitis
10
Immunohistochemical Evaluation of MET in ESCC
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Freshly cut 4-μm sections of each paraffin block of 22 ESCC samples and 19 NTST were used to perform immunohistochemistry using primary antibody against MET (EP1454Y, Abcam®, USA). Antigen retrieval was performed in a water bath while slides were submerged in citrate buffer, pH 6.0, for 40 min at 98°C. The detection was performed following the supplier's recommendations for Novolink™ Max Polymer Detection System (Leica, UK). Samples from lung adenocarcinoma were used as a positive control of MET expression. In the negative control, the primary antibody was replaced with the antibody diluent solution.
Digital images were captured using the Aperio ScanScope CS Slide Scanner (Aperio Technologies, USA) under 20× objective magnification (0.5 μm resolution). An expert pathologist selected the tumor areas using ImageScope software suite (Aperio Technologies). The digital image analysis was performed on whole slide images with Aperio Membrane V9 algorithms (Aperio Technologies). The quantification was done automatically, after algorithm calibration by an experienced observer, and results are reported for MET immunohistochemistry (IHC) as scores from 0 to 3+, and positive tumor cell was defined at the membrane completeness between scores 1 to 3.
Digital images were captured using the Aperio ScanScope CS Slide Scanner (Aperio Technologies, USA) under 20× objective magnification (0.5 μm resolution). An expert pathologist selected the tumor areas using ImageScope software suite (Aperio Technologies). The digital image analysis was performed on whole slide images with Aperio Membrane V9 algorithms (Aperio Technologies). The quantification was done automatically, after algorithm calibration by an experienced observer, and results are reported for MET immunohistochemistry (IHC) as scores from 0 to 3+, and positive tumor cell was defined at the membrane completeness between scores 1 to 3.
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