Breast cancer cell lines were obtained from American Type Culture Collection (ATCC) and HepG2 from LGC Standards GmbH and cultured at 37°C, 5% CO2 in a humidified atmosphere. Cell line identity was authenticated by STR profiling, and all cells were verified to be mycoplasma‐free (Eurofins Genomics). HepG2 (RRID: CVCL_0027) and MCF7 (RRID: CVCL_0031) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) High Glucose with 10% fetal bovine serum (FBS), both from Cytiva/HyClone. Insulin (Thermo Fisher Scientific) was added to 10 μg/mL for MCF7. BT‐474 (CVCL_0179) and T47D (CVCL_0553) were cultured in RPMI‐1640 (Cytiva/HyClone) with 10% FBS (Cytiva/HyClone). For experiments involving stimulation with ER ligands, cells were seeded in phenol red‐free DMEM (Cytiva/HyClone) with 2.5% charcoal‐stripped, dextran‐treated FBS (CSS‐FBS, Cytiva/HyClone).
Bt 474
Manufactured by Cytiva
Sourced in Sweden
The BT-474 is a laboratory instrument designed for cell culture and analysis. It is capable of performing various routine cell culture tasks such as cell counting, cell viability assessment, and cell line maintenance. The BT-474 utilizes advanced optical and digital technologies to provide accurate and reliable data, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Insulin, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by Thermo Fisher Scientific (2 mentions)
Mycoplasma free, by Eurofins (2 mentions)
Fetal bovine serum (fbs), by Cytiva (2 mentions)
Rpmi 1640, by Cytiva (2 mentions)
Hepg2, by LGC (2 mentions)
Hepg2, by Cytiva (2 mentions)
Dmem high glucose medium, by Cytiva (2 mentions)
Css fbs, by Cytiva (2 mentions)
Bt474, by American Type Culture Collection (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
Skov3, by American Type Culture Collection (2 mentions)
Skov3, by Cytiva (2 mentions)
Skbr3, by Cytiva (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Bt474, by Merck Group (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
4 protocols using bt 474
1
Breast and Liver Cancer Cell Culture
2
Breast and Liver Cancer Cell Culture
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Breast cancer cell lines were obtained from American Type Culture Collection (ATCC) and HepG2 from LGC Standards GmbH and cultured at 37°C, 5% CO2 in a humidified atmosphere. Cell line identity was authenticated by STR profiling, and all cells were verified to be mycoplasma‐free (Eurofins Genomics). HepG2 (RRID: CVCL_0027) and MCF7 (RRID: CVCL_0031) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) High Glucose with 10% fetal bovine serum (FBS), both from Cytiva/HyClone. Insulin (Thermo Fisher Scientific) was added to 10 μg/mL for MCF7. BT‐474 (CVCL_0179) and T47D (CVCL_0553) were cultured in RPMI‐1640 (Cytiva/HyClone) with 10% FBS (Cytiva/HyClone). For experiments involving stimulation with ER ligands, cells were seeded in phenol red‐free DMEM (Cytiva/HyClone) with 2.5% charcoal‐stripped, dextran‐treated FBS (CSS‐FBS, Cytiva/HyClone).
3
Cell Line Cultivation Protocol
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AU565, SKBR3, SKOV3, BT474, and A549 cell lines were obtained from American Type Culture Collection (ATCC, via LGC Promochem, Borås, Sweden) and were grown in McCoy’s 5A (SKOV3, SKBR3), RPMI-1640 (AU565, BT474) or Dulbecco’s modified Eagle medium (A549) (Cytiva Hyclone, Uppsala, Sweden) supplemented with 10% FBS (20% FBS for BT474) (Sigma-Aldrich) in a humidified incubator at 37 °C in 5% CO2 atmosphere.
4
Cell Line Cultivation Protocols
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AU565, SKBR3, SKOV3, MCF7, BT474, and A549 cell lines were obtained from the American Type Culture Collection (ATCC via LGC Promochem, Borås, Sweden). They were grown in McCoy’s 5A (SKOV3, SKBR3), RPMI-1640 (AU565, BT474), or Dulbecco’s modified Eagle medium (MCF7, A549) (Cytiva Hyclone, Uppsala, Sweden) in a humidified incubator at 37 °C in 5% CO2 atmosphere. The media were supplemented with 10% fetal bovine serum (FBS) (20% for BT474) (Sigma-Aldrich) and a mixture of penicillin 100 IU/mL and 100 µg/mL streptomycin.
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