Jb6 p and jb6 p cells

JB6 P⁺ and JB6 P⁻ cells (mouse skin epithelial cells) were obtained from the American type Culture Collection (Manassas, VA, USA) and were free from mycoplasma contamination. Cells were grown in MEM (Invitrogen, Carlsbad, CA, USA) supplemented with 5% FBS and antibiotics. NMuMG cells were received as a kind gift from Dr Richard Schwartz (MSU) and were grown in DMEM (Invitrogen) supplemented with 10% FBS and antibiotics. Pharmacological inhibitors against cMyc, mTOR and Erk1 were purchased from EMD Millipore (Billerca, MA, USA). Tivantinib was purchased from APExBIO (Houston, TX, USA). FGFR-1 Ab for immunofluorescence was purchased from Cell Signaling Technology (Danvers, MA, USA). FGF2 Ab was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies: p-mTOR (Cell Signaling Technology #2974), mTOR (Cell Signaling Technology #2983), cMyc (Cell Signaling Technology #5605), p-Erk1 (Cell Signaling Technology #4370), Erk1 (Cell Signaling Technology #9102), Actin (Sigma #A5060), HGF (Abcam #ab-83760), FGF2 (Santa Cruz #sc-365106), FGFR-1 (R&D Systems, Minneapolis, MN, USA #MAB765), Anti-Rabbit 2nd Ab (Li-Cor #926-32213), Anti-Mouse 2nd Ab (Li-Cor #926-32212).