PGE2, Butaprost, PF-04418948 and Iloprost were purchased from Millipore-Sigma. ONO-AE248 and ONO-AE1–329 were gifts from Ono Pharmaceuticals. C52 (Charnwood Molecular) and PF-04418948 (Millipore-Sigma) were resuspended in 40% PEG (Sigma-Aldrich) and 60% of a 30% Kolliphor HS15 solution (Sigma-Aldrich) and administered orally at 10 mg/kg/d and 2.5 mg/kg/d, respectively. U-13C-glucose, U-13C-lactate, U-13C-glutamine and U-13C-pyruvate were purchased from Cambridge Isotopes. HUSH-29 plasmids containing shRNAs to human GYS1 were purchased from Origene Technologies. Human MDMs were incubated with 1640 medium with GlutaMAX + HEPES without sodium pyruvate (Thermo Fisher Scientific, 72400146). Mouse macrophages were incubated in DMEM without sodium pyruvate (Sigma-Aldrich, D5796).
Glutamax hepes
Manufactured by Thermo Fisher Scientific
Sourced in Poland
GlutaMAX + HEPES is a buffer solution designed for cell culture applications. It contains the amino acid glutamine and HEPES, a widely used buffering agent. The product is intended to maintain the pH and nutrient levels in cell culture media.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
U 13c glucose, by Cambridge Isotopes (1 mentions)
U 13c pyruvate, by Cambridge Isotopes (1 mentions)
U 13c glutamine, by Cambridge Isotopes (1 mentions)
U 13c lactate, by Cambridge Isotopes (1 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Sw620, by Thermo Fisher Scientific (1 mentions)
Mycoprobe mycoplasma detection kit, by R&D Systems (1 mentions)
Colo205, by Thermo Fisher Scientific (1 mentions)
Mouse interferon gamma, by Merck Group (1 mentions)
4 protocols using glutamax hepes
1
Investigating Lipid Metabolism Pathways
2
Embryoid Body Formation from Human Embryonic Stem Cells
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Cells were seeded in 200 µl /well of hES-media (Final DMEM-F12 (Thermo Fisher Scientific, 11320074), 20% KSR (Thermo Fisher Scientific, 10828028), 1% Penicillin /Streptomycin, 1% NEAA (Thermo Fisher Scientific, 11140050), 0,5% GlutaMAX, HEPES (Thermo Fisher Scientific, 31330038)), supplemented with final 10 µM Y-27632. Single-cell suspensions were spun at 100 x g for 1 min at RT and further cultured for 16 hr at 37 °C, 5% CO2. The following day 150 µl media supernatant was carefully exchanged by 150 µl fresh hES-media (without Y-27632). Cells were further cultured for additional 48 hr at 37 °C, 5% CO2. The very same media was replaced every 48 hr until day 9. At day 9, EBs were collected, washed once in DPBS and RNA isolated (s. RNA isolation and cDNA synthesis).
3
Cell Line Culture and Authentication
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Cell lines used in this study were purchased
from the American Type Culture Collection via LGC Standards. Human
normal lung fibroblasts (MRC-5), alveolar basal epithelial cell adenocarcinoma
(A549), colorectal adenocarcinoma (Colo205), hepatocellular carcinoma
(HepG2), breast adenocarcinoma (MCF7), and colorectal adenocarcinoma
(SW620) and its MDR variants55 (link) were cultured
in standard conditions (37 °C, 5% CO2, 100% relative
humidity) in high glucose DMEM medium supplemented with GlutaMax,
HEPES (ThermoFisher Scientific) and 10% fetal bovine serum (EURx,
Poland). All cell lines were tested for Mycoplasma contamination using a MycoProbe mycoplasma detection kit (R&D
System).
from the American Type Culture Collection via LGC Standards. Human
normal lung fibroblasts (MRC-5), alveolar basal epithelial cell adenocarcinoma
(A549), colorectal adenocarcinoma (Colo205), hepatocellular carcinoma
(HepG2), breast adenocarcinoma (MCF7), and colorectal adenocarcinoma
(SW620) and its MDR variants55 (link) were cultured
in standard conditions (37 °C, 5% CO2, 100% relative
humidity) in high glucose DMEM medium supplemented with GlutaMax,
HEPES (ThermoFisher Scientific) and 10% fetal bovine serum (EURx,
Poland). All cell lines were tested for Mycoplasma contamination using a MycoProbe mycoplasma detection kit (R&D
System).
4
YAMC Cell Culture Protocol
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YAMC cells were kindly provided by Dr. Robert Whitehead16 (link). Unless otherwise stated, YAMC cells were cultured in RPMI 1640 media containing GlutaMAX, Hepes (Thermo Fisher Scientific, Waltham, MA) and supplemented with 5% fetal bovine serum (Thermo Fisher Scientific), ITS (Corning, Tewksbury, MA) and mouse interferon gamma (Sigma-Aldrich, St.Louis, MO).
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