The 24 h cultured skin biopsies from the ‘bath’ and injection approach were incubated dermal side down on 0.5 m ammonium-isothiocyanate (Merck, Darmstadt, Germany) for 45 min at 37°C. The epidermis was peeled off the dermis and cut into 5 × 5 mm pieces and fixed in acetone for 20 min at room temperature. Targeting mAb was visualized as described above and LC counter-stained with biotinylated CD1a (clone HI149, BioLegend), followed by Strepavidin-Alexa–Fluor 488 (Invitrogen). Evaluation was performed on a conventional Olympus epifluorescence microscope.
Ammonium isothiocyanate
Manufactured by Merck Group
Sourced in Germany
Ammonium-isothiocyanate is a chemical compound used in laboratory settings. It serves as a precursor for the synthesis of other chemical compounds. The compound's core function is to provide a source of isothiocyanate, which is a reactive functional group utilized in various chemical reactions and experiments.
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2 protocols using ammonium isothiocyanate
1
Epidermal Langerhans Cell Visualization
2
Immunofluorescent Epidermal-Dermal Analysis
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The 24 h cultured skin biopsies from the ‘bath’ and injection approach were incubated dermal side down on 0.5 m ammonium-isothiocyanate (Merck, Darmstadt, Germany) for 45 min at 37°C. The epidermis was peeled off the dermis and cut into 5 × 5 mm pieces and fixed in acetone for 20 min at room temperature. Targeting mAb was visualized as described above and LC counterstained with biotinylated CD1a (clone HI149, BioLegend), followed by Strepavidin-Alexa–Fluor 488 (Invitrogen). Evaluation was performed on a conventional Olympus epifluorescence microscope.
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