Pe conjugated anti human cd8

Manufactured by BD

Sourced in United States

PE-conjugated anti-human CD8 is a monoclonal antibody that binds to the CD8 surface receptor expressed on certain T-cells. The antibody is labeled with the fluorescent dye phycoerythrin (PE), enabling detection and analysis of CD8-positive cells using flow cytometry.

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Lab products found in correlation

Fitc conjugated anti human cd4, by BD (2 mentions) Flow cytometry, by BD (1 mentions) Biochemical autoanalyzer, by Beckman Coulter (1 mentions) Cobas amplicor hiv 1 monitor test kit, by Roche (1 mentions) Accuri c6 plus, by BD (1 mentions) Ab8216, by Abcam (1 mentions) Pe conjugated anti human cd33, by BioLegend (1 mentions) Apc conjugated anti human cd45, by BD (1 mentions) Fitc conjugated anti mouse cd45, by BD (1 mentions) Pe conjugated anti human cd19, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Pe conjugated anti human cd3, by BD (1 mentions)

Close spelling variants of this product

Anti-CD8 (193 citations) CD8-PE (128 citations) Anti-CD8-PE (95 citations) Anti-human CD8-PE (14 citations) PE-conjugated anti-CD8 (12 citations) Anti-human CD8 (10 citations)

3 protocols using pe conjugated anti human cd8

Biomarkers for HIV-1 Infection Assessment

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Patient blood samples were drawn after a 12-h fast within 3 days of admitted to hospital. The serum sodium concentration was assayed with a biochemical autoanalyzer (Beckman Coulter, CA, USA) using an ion-selective electrode method. The numbers of CD4+ and CD8+ T cells were measured by flow cytometry (B&D Science, NJ, USA) following staining with FITC-conjugated anti-human CD4, PE-conjugated anti-human CD8, and PE-Cy5 conjugated anti-human CD3 mAbs (B&D Sciences, NJ, USA). HIV-1 RNA was assayed according to the standard protocol of the COBAS Amplicor HIV-1 Monitor Test kit (Roche, IN, USA) on the COBAS Amplicor PCR system (version 1.5) (Roche, IN, USA).

Xu L., Ye H., Huang F., Yang Z., Zhu B., Xu Y., Qiu Y, & Li L. (2014). Moderate/Severe Hyponatremia Increases the Risk of Death among Hospitalized Chinese Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome Patients. PLoS ONE, 9(10), e111077.

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Immunophenotyping of Humanized Mice

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Blood, spleen, and BM cells from hu-mice were stained with antibodies and analyzed using a BD Accuri C6 Plus (BD Biosciences). The antibodies used to detect human cells included APC-conjugated anti-human CD45 (555485, BD Pharmingen^TM), FITC-conjugated anti-mouse CD45 (553080, BD Pharmingen^TM), APC-conjugated anti-human CD4 (555349, BD Pharmingen^TM), PE-conjugated anti-human CD8 (555367, BD Pharmingen^TM), PE-conjugated anti-human CD19 (302208, BioLegend), and PE-conjugated anti-human CD33 (303403, BioLegend). For immunocy-tochemistry, anti-CD45 (ab8216, Abcam) and anti-CD34 (MAB72271, R&D Systems) antibodies were used, and proper isotype-matched IgG antibodies were used to detect the primary signals. Flow cytometric data were analyzed using the CSampler^TM Plus software program (BD Bio-sciences).

Han A.R., Lee J.E., Lee M.J., Ko S.Y., Shin H.S., Lee J.Y, & Lee D.R. (2021). Distinct Repopulation Activity in Hu-Mice between CB- and LPB-CD34＋ Cells by Enrichment of Transcription Factors. International Journal of Stem Cells, 14(2), 203-211.

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Phenotypic Analysis of Immune Cell Infiltration

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The frozen blocks were sectioned (8-μm-thick) for immunostaining. PEconjugated anti-human CD3, PE-conjugated anti-human CD8, and FITC-conjugated anti-human CD4 antibodies (all from BD Biosciences) were applied overnight at 4°C. The slides were washed and stained with DAPI to evaluate the nuclei. The slides were observed by fluorescence microscopy. To quantify tissue infiltration of CD4 or CD8 T cells, the number of markerpositive cells for each subset within the cross-section of the tissues was measured using Image J 1.50i software. To stain live HUVECs in the gel tissues, paraffin blocks were sectioned (5-μmthick) and incubated with FITC-conjugated ULEX (Vector Lab., Inc.) overnight at 4°C. The slides were washed and stained with DAPI (Thermo Fisher Scientific) to evaluate the nuclei. The slides were observed by fluorescence microscopy.

Lim S., Kirkiles-Smith N.C., Pober J.S., Bothwell A.L, & Choi J.M. (2018). Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection. Biomaterials, 183, 128-138.

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