Hplc grade chloroform and poly lactide co glycolic acid

The ingredients for nanoparticle preparation were purchased as follows. 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), Cholesterol and Maleimide-terminated PEG-DSPE (Mal-PEG) from Avanti Polar lipids Inc. (Birmingham, AL, USA), Protamine sulfate salt Grade X, HPLC grade chloroform and Poly (lactide-co-glycolic acid) or PLGA from Sigma Chemical Co. (St. Louis, MO, USA). The cell culture reagents Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Lonza, Fetal bovine serum albumin (BSA) from Mediatech, and penicillin/streptomycin antibiotics were purchased from Gibco, Invitrogen Corp. (Carlsbad, CA, USA). The primary antibody for P-glycoprotein was from Pierce, Thermo Scientific (Waltham, MA) and β-actin from Sigma Chemical Co. (St. Louis, MO, USA). The anti-mouse HRP-labeled secondary antibody was purchased from GE Healthcare (Little Chalfont, UK). The MTT reagent was supplied by Sigma Chemical Co. (St. Louis, MO, USA).