We used 8 glioma cell lines, namely U87MG, A172, LN405, SW1783, T98G, SW1088, CCF-STTG-1, and Gos-3. Cell lines U87MG, A172, and T98G were purchased from the European Collection of Cell Cultures (Salisbury, Wiltshire, UK). Cell lines SK-PN-DW, CCF-STTG1, SW1088, and SW1783 were purchased from the American Type Culture Collection (Manassas, VA, USA). Cell lines LN405 and Gos-3 were obtained from the Deutsche Sammlung Von Mikroorganismen und Zellkulturen (Braunschweig, Germany). Cell lines were cultured in RPMI L-Glutamax medium (GIBCO-BRL, Gaithersburg, MD, USA), supplemented with 10 % fetal bovine serum (FBS), 10 % non-essential amino acids (NEAs), 1 % penicillin, and 0.1 % amphotericin B. Cell lines were maintained in a 37 °C incubator with the supply of 5 % CO2. Sub-culturing of cells was performed after 80 % confluence with the help of trypsin/EDTA 1X.
Gos 3
Manufactured by Leibniz Institute DSMZ
Sourced in Germany
The Gos-3 is a laboratory equipment designed for the cultivation and analysis of microbial cultures. Its core function is to provide a controlled environment for the growth and observation of various microorganisms. The Gos-3 enables researchers to monitor and maintain optimal conditions for microbial cultures, facilitating scientific investigations and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Sw1088, by American Type Culture Collection (2 mentions)
Sw1783, by American Type Culture Collection (2 mentions)
Sk pn dw, by American Type Culture Collection (1 mentions)
Ccf sttg1, by American Type Culture Collection (1 mentions)
U87mg, by European Collection of Cell Cultures (1 mentions)
U87mg, by American Type Culture Collection (1 mentions)
U251mg, by American Type Culture Collection (1 mentions)
Human hek293t, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Xav939, by Selleck Chemicals (1 mentions)
2 protocols using gos 3
1
Glioma Cell Line Culture Protocol
2
Glioma Cell Culture and XAV-939 Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glioma cell lines SW1783, U87MG, SW1088, LN18, T98G and U251MG, and human HEK293T cells were purchased from the American Type Culture Collection (ATCC). Glioma cell lines GMS-10 and GOS-3 were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). All cell lines were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, USA) medium supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 100 units/mL penicillin and 100 units/mL streptomycin in a humidified atmosphere at 37 ˚C with 5% CO2. XAV-939 (#S1180) was purchased from Selleck Chemicals (USA) and dissolved in dimethylsulfoxide (DMSO).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!