Hucs10p

miRNA122a negative HCC cell lines Hep3B, PLC/PRF/5, SKHep1, and SNU423 were obtained from ATCC and maintained in DMEM (Thermo Fisher Scientific, Scoresby, Australia) media supplemented with 10% fetal bovine serum (FBS) (Gibco, Australia) and 1% penicillin/streptomycin (P/S) (Gibco). miRNA122a positive HCC cell line HuH7 was provided by Dr. Kim Bridle (University of Queensland). Cell line ID service was provided by the Australian Genome Research Facility (AGRF) and cell line genotyping was performed to confirm identity of cell lines. Breast cancer cell line T47D and prostate cancer cell lines LNCaP and DU145 were maintained in DMEM media with 10% FCS and 1% P/S. Melanoma cell lines 92.1, Mel202, Mel270 (gifted by Nicholas Hayward) and ovarian cancer lines SKOV3, CAOV3, TOV21G were cultured in RPMI media (Thermo Fisher Scientific) supplemented with 10% FCS and 1% P/S. Human retinal pigmental epithelium cell line ARPE-19 was maintained in DMEM/F12 media (Thermo Fisher Scientific) with 10% FCS and 1% P/S. Cryopreserved human hepatocytes (HUCS10P) (Lonza, Australia) containing pooled hepatocytes from 10 donors were cultured as per the manufacturer's recommendations in human hepatocyte maintenance media.

The LO-2 human normal hepatic cell line and the QGY-7701 and HUH7 HCC cell lines were obtained from ATCC. The HepG2, Bel-7404, SK-Hep-1, and Hep3B HCC cell lines were obtained from the National Collection of Authenticated Cell Cultures (NCAC) in China. Primary human hepatocytes (HH, mixed donor) were obtained from Lonza (HUCS10P). Cell lines were cultured with Eagle's minimum essential medium (EMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco). HH cells were cultured in HCM™ Hepatocyte Culture Medium BulletKit™ (CC-3198, Lonza). All cells were incubated at 37°C and 5% CO₂. hsa-miR-582-5p knockout (miRKO) cell lines were generated by GenePharma with the CRISPR/Cas9 system.