All animal experiments were carried out according to the guidelines of the German Regulations for Animal Welfare and have been approved by the local Ethical Committee for Animal Experiments. A number of 4×106 MPC or MTT cells were re-suspended in 40 µL of Dulbecco's phosphate-buffered saline and injected subcutaneously into the shoulder of 10-15 week-old female nude mice (Rj:NMRI-Foxn1nu/nu, Janvier Labs, Le Genest-Saint-Isle, France). Prior to imaging procedures, general anesthesia was induced and maintained with inhalation of 10% (v/v) desflurane in 30/10% (v/v) oxygen/air. During anesthesia, animals were continuously warmed at 37°C. Tumor growth was monitored three times per week using caliper measurements. Tumor volume was calculated assuming a tri-axial ellipsoid with the axes a, b, and c using the formula V = π/6×abc. Animals were sacrificed using CO2 inhalation and cervical dislocation.
Rj nmri foxn1nu nu
Manufactured by Janvier Labs
Sourced in France
The Rj:NMRI-Foxn1nu/nu is a laboratory equipment designed for specific research applications. It serves as a tool for conducting experiments, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Further information may be available from the manufacturer or subject matter experts.
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5 protocols using rj nmri foxn1nu nu
1
Subcutaneous Tumor Xenograft Model in Nude Mice
2
In Vivo Functionality of RevCAR T-Cells
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All animal procedures have been approved by the local ethics committee for animal experiments (Landesdirektion Dresden, 24–9165.40-4/2013, 24.9168.21–4/2004-1) and were performed at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) in accordance with the German regulations of animal welfare. Five-week-old male NMRI-nude immunodeficient mice (Rj:NMRI-Foxn1nu/nu) (Janvier Labs, Le Genest-Saint-Isle, France) were randomly (unblinded) assigned to experimental groups of five animals and housed in a pathogen free facility with 12 h light/dark cycle. The health status of the mice was supervised daily by husbandry personnel. To assess in vivo functionality of RevCAR T-cells, 1 × 106 MOLM-13-Luc cells were injected subcutaneously alone or mixed with RevCAR T-cells (1 × 106) and RevTM (17 µg) in a total volume of 100 µL PBS (Thermofisher Scientific). The optical imaging was performed with the In-Vivo Xtreme (Bruker, Nehren, Germany) as described before [15 (link),25 (link)].
3
SPECT Imaging and Biodistribution of PC3 Cells
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All animal handling was performed according to the guidelines of the German Regulations of Animal Welfare. The protocol was approved by the local Ethical Committee for Animal Experiments (reference number 24-9168.21-4/2004-1). For SPECT imaging, an amount of 4 × 106 PC3 and 3 × 106 PC3-P cells was injected subcutaneously in the left and right flank, respectively, of athymic NMRI nude mice (Rj:NMRI-Foxn1nu/nu; Janvier Labs, Le Genest-Saint-Isle, France). For biodistribution, mice were injected with PC3-P cells on their right flank only. Subcutaneous tumors were allowed to grow for about 21 days to reach a tumor size of about 500 mm3. Before radioactivity application the mice weighed 23 ± 1.9 g for biodistribution (n = 8) or 27 ± 1.7 g for SPECT imaging (n = 2).
4
In vivo Biodistribution of Barium Radioisotopes
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Animal experiments were performed at HZDR according to the guidelines of German Regulations for Animal Welfare and have been approved by the local Animal Ethics Committee for Animal Experiments (Landesdirektion, Dresden, Germany). Investigations were performed using female athymic nude mice (Rj:NMRI-Foxn1nu/nu, Janvier Labs, Le Genest-Saint-Isle, France), between 12 and 14 weeks old, housed in a pathogen-free facility. Animals received one intravenous injection (0.1–0.2 mL; tail vein) of [131Ba]Ba(NO3)2 diluted in 0.01 M HNO3, pH 6, or 131Ba-macropa (0.1–0.2 mL in 0.2 M ammonium acetate; pH 6), respectively. For in vivo imaging, anesthesia was induced and maintained with inhalation of 10 vol% desflurane (Baxter, Deerfield, IL, USA) in 30 vol% oxygen air, and body temperature of the animals was maintained at 37 °C. After the last imaging examination and during biodistribution studies, animals were sacrificed using CO2 inhalation and cervical dislocation, and organs were excised for further analyses.
5
Orthotopic Glioblastoma Xenograft Model
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Female athymic nude mice (Rj:NMRI-Foxn1 nu/nu) were chosen for this study (Janvier labs, France). The mice were used for tumour implantation at the age of 8 weeks (26–30 g). During microsurgery mice were anesthetized with a mixture of air and isoflurane concentrate (1.5–2% depending on the breathing) under sterile conditions. The mice were placed into a Stoelting stereotactic frame (just for mouseTM, Stoelting Europe, Dublin, Ireland). A midline incision was done and a burr hole was drilled 0.5 mm anterior and 2.5 mm lateral to the bregma. 5 × 104 U87-MG cells were suspended in 1 μL Hank’s Buffered Salt Solution (HBSS, 1X) and were injected 3.0 mm into the brain parenchyma with a flow of 0.1 μL/min using a 10 μL Hamilton syringe. After injection, the burr hole was filled with bonewax (Ethicon, US, LLC), the scalp incision sutured (Vicryl 6.0, Ethicon, US, LLC) and the surface antiseptically cleaned. Animal sacrifice was performed by induction of anesthesia with a mixture of air and isoflurane concentrate followed by cervical dislocation.
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