Anti ddb1

Manufactured by BD

Anti-DDB1 is a laboratory reagent used for the detection and analysis of the DDB1 protein. DDB1 is a crucial component of the nucleotide excision repair pathway, which is responsible for repairing DNA damage. Anti-DDB1 can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression, localization, and interactions of the DDB1 protein.

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Lab products found in correlation

Anti ki67, by Thermo Fisher Scientific (1 mentions) Anti β catenin, by Merck Group (1 mentions) Anti sox2, by Merck Group (1 mentions) Anti cul4b, by Merck Group (1 mentions) Ab5603, by Abcam (1 mentions) Anti ctip, by Abcam (1 mentions) Anti histone 4, by Abcam (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti actin, by Merck Group (1 mentions) Anti gapdh, by Merck Group (1 mentions) Anti arp3, by Santa Cruz Biotechnology (1 mentions) Anti pericentrin, by Fortrea (1 mentions) Immobilon p membrane, by Merck Group (1 mentions) Imagequant las4010 biomolecular imager, by GE Healthcare (1 mentions) Anti ha 3f10, by Roche (1 mentions)

2 protocols using anti ddb1

Comprehensive Antibody Panel for Western Blot and Immunofluorescence

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For western blot and immunofluorescence analysis, following primary antibodies were used: Anti‐Actin (mouse, Merck Millipore); Anti‐ARP3 (mouse, Santa Cruz); Anti‐CK1α (mouse, Santa Cruz); Anti‐CUL4A (rabbit); Anti‐CUL4‐CT (rabbit) (Olma et al, 2009 (link)); Anti‐CUL4B (rabbit, Sigma); Anti‐DCTN1 (rabbit, Atlas antibodies); Anti‐DDB1 (mouse, BD Biosciences); Anti‐GAPDH (mouse, Sigma); Anti‐HA.II (mouse or rabbit, both Covance); Anti‐Histone 4 (rabbit, Abcam); Anti‐Phospho‐Histone H2A.X S139 (mouse, Merck Millipore); Anti‐Phospho‐Histone H3 S10 (rabbit, Upstate); Anti‐LIS1 (mouse, Santa Cruz Biotechnology); Anti‐Pericentrin (rabbit, Covance); Anti‐Tubulin (mouse, Sigma); Anti‐WDR1 (rabbit, Atlas antibodies); Anti‐SOX2 (rabbit, Millipore, AB5603); Anti‐CTIP (rat, Abcam, ab18465); Anti‐β‐catenin (mouse, Millipore, AB5733); and Anti‐Ki67 (rat, eBioscience, 14‐5698‐82). For western blot Anti‐mouse or rabbit IgG‐HRP (both Bio‐rad) and for immunofluorescence anti‐mouse or rabbit IgG‐Alexa 488, 568, and 647 (Invitrogen), secondary antibodies were used. Rhodamine phalloidin dye was used for acting staining.

Stier A., Gilberto S., Mohamed W.I., Royall L.N., Helenius J., Mikicic I., Sajic T., Beli P., Müller D.J., Jessberger S, & Peter M. (2023). The CUL4B‐based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho‐specific DCAFs. The EMBO Journal, 42(17), e112847.

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Western Blot and Immunofluorescence Analysis of DNA Damage Response Proteins

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Anti-XPC (13 (link)) and anti-RAD23B (39 (link)) antibodies were obtained as described previously. Anti-lamin B1 and CUL4 (Santa Cruz Biotechnology), anti-DDB1 (BD Biosciences), anti-DDB2 (R&D Systems) and anti-HA (3F10: Roche Applied Science) antibodies were purchased, respectively. For immunoblot analyses, proteins separated by SDS-PAGE were transferred onto Immobilon-P membranes (Merck Millipore) and detected by chemiluminescence using the appropriate secondary antibodies and substrates. Detection and quantitation were performed on an ImageQuant LAS-4010 biomolecular imager (GE Healthcare Biosciences). Blots were also exposed to X-ray films. Immunofluorescence staining was carried out basically as described previously (40 (link)).

Matsumoto S., Fischer E.S., Yasuda T., Dohmae N., Iwai S., Mori T., Nishi R., Yoshino K.I., Sakai W., Hanaoka F., Thomä N.H, & Sugasawa K. (2015). Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein. Nucleic Acids Research, 43(3), 1700-1713.

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