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Anti cd40 clone 1c10

Manufactured by Thermo Fisher Scientific
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The Anti-CD40 (Clone 1C10) is a lab equipment product. It functions as an antibody that binds to the CD40 antigen.

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Lab products found in correlation

E coli lps, by InvivoGen (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Anti-CD40 (70 citations) Anti-CD40 (1C10) (5 citations) CD40 (clone 1C10, (2 citations) CD40 (1C10, (2 citations)

2 protocols using anti cd40 clone 1c10

1

B Cell Stimulation Protocol

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Mice were sacrificed by CO2 inhalation according to a CWRU-approved IACUC protocol, and blood was obtained via cardiac blood draw into 3.2% sodium citrate to prevent coagulation. Plasma was generated from blood by taking the liquid fraction following centrifugation at 2500 x g for 15 minutes. B cells were obtained as described and plated in round-bottom 96-well plates at a density of 100,000 cells/well. The cells were stimulated by the addition of 100 ng/mL anti-CD40 (Clone 1C10; eBioscience) and 1 ng/mL E. coli LPS (Invivogen) to the media, and incubated for 7 days prior to media harvest.
Glendenning L.M., Zhou J.Y., Reynero K.M, & Cobb B.A. (2022). Divergent Golgi Trafficking Limits B cell-Mediated IgG Sialylation. Journal of leukocyte biology, 112(6), 1555-1566.
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2

Anti-CD19 and Anti-CD40 Immune Modulation

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Six-week-old C57BL/6 mice (The Jackson Laboratory) were injected i.v. with 500 μg/kg of anti-mCD19 or isotype control antibody. Mice were given 1.5 mg/kg of anti-CD40 (clone 1C10, eBioscience, 16-0401-86) via retroorbital vein either 1 hour after the i.v. injection (before washout) or 4 weeks after the i.v. injection (after washout). Mice were euthanized 24 hours after anti-CD40 injections; splenocytes were analyzed by FACS.
Boyles J.S., Sadowski D., Potter S., Vukojicic A., Parker J., Chang W.Y., Ma Y.L., Chambers M.G., Nelson J., Barmettler B., Smith E.M., Kersjes K., Himes E.R., Lin C., Lucchesi J., Brahmbhatt J., Sina R., Martin J.A., Maestri E., Wiethoff C.M., Dyas G.L., Linnik M.D., Na S., Witcher D.R., Budelsky A, & Rubtsova K. (2023). A nondepleting anti-CD19 antibody impairs B cell function and inhibits autoimmune diseases. JCI Insight, 8(13), e166137.
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