Pdnor207

Manufactured by Thermo Fisher Scientific

Sourced in United States

The PDNOR207 is a versatile laboratory instrument designed for precise and reliable measurements. It features advanced technology to ensure accurate data collection and analysis. The core function of this product is to provide a robust and dependable platform for various laboratory applications.

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Lab products found in correlation

Bp reaction, by Thermo Fisher Scientific (2 mentions) Lr reaction, by Thermo Fisher Scientific (2 mentions) Tcs sp8 microscope, by Leica (1 mentions) Bp reaction kit, by Thermo Fisher Scientific (1 mentions) Tcs sp8, by Leica (1 mentions)

2 protocols using pdnor207

Subcellular Localization of ZEP1 Protein

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We cloned ZEP1 cDNA from Kronos, ligated it into the pDNOR207 vector by BP reaction (Invitrogen, Life Technologies, Carlsbad, CA, USA), and confirmed the fragment by sequencing (Supplemental Table 2). The GUS gene was from the control of the BP reaction kit (Invitrogen). For subcellular localization analysis, we incorporated the genes into pMDC83 to create Pro_35S:ZEP1-GFP or Pro_35S:GUS-GFP by LR reaction (Invitrogen), transformed the constructs into Agrobacterium GV3101, and infiltrated the cells into tobacco leaves for 48 h before observing the leaves under a Leica TCS SP8 microscope (Leica Microsystems, Mannheim, Germany). We captured images with 488-nm laser excitation and 500- to 550-nm long-pass emission filters. We imaged chloroplast autofluorescence with 633-nm laser excitation and 640- to 700-nm long-pass emission filters.

Chang C.Y., Yang S.X., Zhang M.Q., Guo Y.T., Li X.M., Yan Y., Ding C.H., Niu K.X., Wang M.L., Li Q.Q., Zhang J., Zhang X., Chen S., Xie C., Ni Z., Sun Q, & Gou J.Y. (2023). Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat. Plant Communications, 4(5), 100608.

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Cloning and Subcellular Localization of KAT-2B

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KAT-2B cDNA was cloned from WT plants, ligated into pDNOR207 by BP reaction (Invitrogen, Life Technologies, Carlsbad, CA, USA), and confirmed by sequencing. For subcellular localization analysis, KAT-2B was incorporated into pMDC43 (Pro_35S: GFP-KAT-2B) by LR reaction (Invitrogen), transformed into Agrobacterium GV3101, infiltrated into tobacco leaves, and observed with an inverted confocal microscope equipped with a ×40 oil-corrected objective in Leica TCS SP8 (Leica, Microsystems, Mannheim, Germany) at 48 h^{46 (link)}. To complement the Arabidopsis kat2 mutant, KAT-2 was incorporated into a gateway transgenic vector under the drive of maize ubiquitin (Pro_Ubi: KAT-2-Flag) by LR reaction, and transformed into Arabidopsis by the flower dip method using Agrobacterium GV3101. Immature embryos were collected and inoculated with Agrobacterium to generate transgenic wheat according to earlier reports^{47 (link)}.

Chen Y., Yan Y., Wu T.T., Zhang G.L., Yin H., Chen W., Wang S., Chang F, & Gou J.Y. (2020). Cloning of wheat keto-acyl thiolase 2B reveals a role of jasmonic acid in grain weight determination. Nature Communications, 11, 6266.

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