The mean diameter of liposomes was determined by dynamic light scattering at 25 °C at an angle of 90°. The zeta potential was evaluated by electrophoretic mobility determination at an angle of 90°. Size and zeta potential measurements were performed in triplicate using the nano ZS90 Zetasizer equipment (Malvern Instruments, England). The samples were diluted using a 0.9% (w/v) NaCl solution. The encapsulation efficiency (EE) of IRN in liposomes was determined by HPLC, using phosphate buffer: acenonitrile (70:30 v/v) + 0.2% triethylamine (pH 2.5, adjusted with phosphoric acid) as the mobile phase, and a reversed-phase C18 column with fluorescence detection [30 (link)].
Nano zs90 zetasizer equipment
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Nano ZS90 Zetasizer is a lab equipment designed for particle size and zeta potential measurements. It utilizes dynamic light scattering (DLS) technology to determine the size distribution of particles in the nanometer to micrometer range. The equipment also measures the zeta potential, which provides information about the surface charge of the particles.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nano zs90 zetasizer equipment
1
Liposomal Encapsulation Efficiency Determination
2
Liposomal Encapsulation Efficiency Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mean diameter of liposomes was determined by dynamic light scattering at 25 °C at an angle of 90°. The zeta potential was evaluated by electrophoretic mobility determination at an angle of 90°. Size and zeta potential measurements were performed in triplicate using the nano ZS90 Zetasizer equipment (Malvern Instruments, England). The samples were diluted using a 0.9% (w/v) NaCl solution. The encapsulation efficiency (EE) of IRN in liposomes was determined by HPLC, using phosphate buffer: acenonitrile (70:30 v/v) + 0.2% triethylamine (pH 2.5, adjusted with phosphoric acid) as the mobile phase, and a reversed-phase C18 column with fluorescence detection [30 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!