Antibodies against gapdh

Cells were harvested by Tyrisin (Invitrogen), then cytoplasmic and nucleus protein was extracted by Cytoplasmic and Nucleus Protein Extraction Kit (Thermal Scientific, USA) according to its protocol, quantified by the BCA assay.
The standard detail experimental process of western blot as same as our previous study (PMID29331423 and PMID29800682). Western blot band was quantified through the Image-J software (NIH, USA). Antibodies against GAPDH, FHL3, E-cadherin, MMP2 and GSK3β were purchased from Proteintech (1:1000, Hangzhou, China), antibodies against smad2/3, smad4, twist1, Ubiquitin, Histone 3, ser473-phospho-Akt, try216/279-phospho-GSK3β, ser9-phospho-GSK3β and ser423/425-phospho-smad2/3 were purchased from Huabio (1:1000, Hangzhou, China), antibodies against ZO-1, Akt, vimentin, snail1 and zeb1 were gained from Abcam (1:1000, China).

Cells were harvested by cytology brush and lysed with RIPA lysis buffer (Sigma, USA) supplemented with phosphorylase and protease inhibitor mixture (Thermo, USA), quantified by the BCA assay. The standard detail experimental process of western blot was the same as our previous study (PMID:31935687). Western blot band was quantified through the Image-J software (NIH, USA). Antibodies against GAPDH, LAPM2 and p62 were purchased from Proteintech (1:1000, China), antibodies against Alex, c-myc, CD63, β-actin, BAX, BCL-2, LC3B, caspase3, caspase7, caspas8, caspas9, caspase12 and cleaved-PARP were purchased from Abcam (1:1000, China).

Human LUAD cell lines, including H1650, H358, HCC827, H23, A549, H1693, H1299 were purchased from American Type Culture Collection (ATCC). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (KeyGen Biotech Co. Ltd., Nanjing, China) with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific, Waltham, MA, U.S.A.) and 1% penicillin and streptomycin (KeyGen Biotech Co. Ltd., Nanjing, China) in a humidified incubator at 37°C containing 5% CO₂. Real-time quantitative PCR primers were provided by Bioengineering. In addition, FastQuant RT Kit (WithgDNase) (Cat:#KR106-02), RNA Extraction Kit (Cat:#RK123), and SuperReal PreMix Plus (SYBR Green) Kit (Cat:#FP205-02) were purchased from TIANGEN. Lipofectamine 2000 transfection reagents were purchased from Invitrogen (Cat:#1854323). Antibodies against GAPDH (Cat:#60004-1-Ig) was purchased from Proteintech and STEAP1 antibody (Cat#88677) was purchased from Cell Signaling Technology. EdU cell proliferation test kit was bought from Solarbio (Cat:#1170).

Antibodies against Flag (catalog no. F1804) and hemagglutinin (HA) (catalog no. H6908) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against IFNAR1 (catalog no. ab45172) and NSE1 (catalog no. ab139689) were purchased from Abcam (Cambridge, MA, USA). Antibody against IRAK1 (catalog no. 4504S) was purchased from Cell Signaling Technology (CST) (Beverly, MA, USA). Antibodies against GAPDH (catalog no. 60004-1-Ig), GFP (catalog no. 66002-1-Ig), GST (catalog no. 66001-1-Ig), SMC5 (catalog no. 14178-1-AP), SMC6 (catalog no. 14465-1-AP), and PJA1 (catalog no. 17687-1-AP) were purchased from Proteintech Group (Wuhan, Hubei, China). Antibody against NSMCE4A (catalog no. AP9909a) was purchased from Abgent (San Diego, CA, USA).
Hepatitis B virus antigen detection ELISA kits were purchased from Ke Hua Bio Engineering (Shanghai, China). The inhibitors Braco19 and MG132 were purchased from Sigma-Aldrich. 3-Deazaneplanocin A (DZNeP), 5-aza-2ʹ-deoxycytidine (5′-Aza), GSK343, idarubicin HCl, and topotecan HCl were purchased from Selleck Chemicals (Houston, TX, USA).

HEK293T, HepG2.2.15, and HepG2 cells were maintained in DMEM medium containing 10% inactivated fetal bovine serum. HLCZ01 cells were maintained according to the previous report.^{26 (link)} All cell lines were maintained in penicillin (100 IU/mL) and streptomycin (100 mg/mL) in the presence of 5% CO₂ at 37°C. The expression construct of CBFβ was generated by cloning the coding region sequence into the VR1012 expression vector, and CBFβ mutants were generated by Quik-Change PCR (TransGen, Beijing, China) (Table S1). The pHBV1.3 plasmid was kindly supplied by Dr. Lishan Su at the University of North Carolina, and the pHBV1.2 WT and ΔX plasmids were kindly provided by Professor Qiang Deng of Fudan University. Antibodies against tubulin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against CBFβ were from Cell Signaling Technology (Danvers, MA). Antibodies against GAPDH were obtained from Proteintech. IFIT2 antibodies were obtained from Signalway Antibody. Smc6 antibodies were purchased from Abcam (Shanghai, China). Human IL-10, IL-29 (IFN-λ1) recombinant protein, and IL-10 and IL-29 neutralized antibodies were obtained from R&D SYSTEMS (Minneapolis, MN, USA). Human IL-10 and IL-29 (IFN-λ1) ELISA Kits were purchased from ORIGENE (USA).