MS-grade acetonitrile (ACN) was purchased from Thermo Fisher Scientific, United States, and ultrapure water was filtered through the Milli-Q system (Millipore, Billerica, MA). Formic acid (FA) was obtained from DIMKA, and ammonium bicarbonate was purchased from Fluka (Honeywell Fluka, United States). Sodium dodecyl sulfate (SDS), SDS-free protein lysate, dithiothreitol (DTT), iodoacetamide (IAM), Coomassie brilliant blue G-250, trypsin and acetone were obtained from Aladdin, China.
Ms grade acetonitrile acn
Manufactured by Thermo Fisher Scientific
Sourced in United States
MS-grade acetonitrile (ACN) is a high-purity organic solvent commonly used in mass spectrometry (MS) applications. It is a clear, colorless liquid with a characteristic sweet odor. MS-grade ACN is designed to meet the stringent requirements of MS analysis, ensuring low levels of impurities and contaminants that could interfere with sensitive MS measurements.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (2 mentions)
Methanol meoh, by Thermo Fisher Scientific (1 mentions)
L leucine d3, by LGC (1 mentions)
L tryptophan d5, by Merck Group (1 mentions)
Progesterone 2 3 4 13c3, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Sucrose, by Bio-Rad (1 mentions)
D glucose, by Bio-Rad (1 mentions)
Cho k1, by American Type Culture Collection (1 mentions)
Simponi, by Johnson & Johnson (1 mentions)
4 protocols using ms grade acetonitrile acn
1
Optimized Protein Sample Preparation
2
LC-MS Metabolite Quantification Standards
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MS-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Thermo Fisher Scientific, USA. Formic acid was obtained from DIMKA, and ammonium formate was purchased from Fluka (Honeywell Fluka, USA). Ultrapure water was filtered through the Milli-Q system (Millipore, Billerica, MA). The internal standard (IS) mixture contained L-Leucine-d3 (Toronto Research Chemicals, TRC), L-Phenylalanine (13C9, 99%) (Cambridge Isotope Laboratories, CIL), L-Tryptophan-d5 (TRC), Progesterone-2,3,4-13C3 (Sigma).
3
Mandarin Fish Protein Extraction
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Mandarin fish (Siniperca chuaisi) were bought in a Yonghui supermarket (Hangzhou, China). Pepsin and bicinchoninic acid (BCA) protein assay kits were purchased from Sigma (St. Louis, MO, USA). Anti-rabbit IgG peroxidase-conjugated antibody produced in goat was purchased from Polila (Beijing, China). 3,3′,5,5′-Tetramethylbenzidine (TMB) was purchased from Aladdin (Shanghai, China). Formic acid (FA) and acetonitrile (ACN) (MS grade) were bought from Thermo Fisher Scientific (Waltham, MA, USA). D-glucose, maltose, sucrose and other chemicals were purchased from Bio-Rad in the best available purity grade.
4
Golimumab Characterization and Cell Assays
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Golimumab A and golimumab B were manufactured by Shanghai Zhangjiang Biotechnology Co., and purified by Protein A Sepharose (Shanghai Zhangjiang Biotechnology Co.). SIMPONI® (golimumab, Janssen) was obtained from the market of Shanghai, China. Acetonitrile (ACN, MS grade) was obtained from Thermo Scientific (United States); ammonium formate (NH4FA), ammonium bicarbonate (NH4HCO3), sodium cyanotrihydridoborate (NaBH3CN), fibrinopeptide (GFP), tris (2-carboxyethyl) phosphine hydrochloride (TCEP), and NaI were all obtained from Sigma (United States). Dithiothreitol (DTT), formic acid (FA, MS grade), and iodoacetamide (MIA) were obtained from Fluka (GER). Trypsin, IdeS (immunoglobulin-degrading enzyme of Streptococcus pyogenes), and recombinant human TNFα were obtained from Shanghai Zhangjiang Biotechnology Co. The CHO-K1 and the mouse fibroblastic cell line L929 cell lines were obtained from the American Type Culture Collection (ATCC).
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