Ten milliliter of blood was drawn from each participant after an overnight fast
between the 2nd and the 3rd day of her menstrual cycle.
Serum samples were carefully collected and stored at -40°C until
analysis. Glucose and lipid profiles, including the TGs, total cholesterol (TC),
very low-density lipoprotein (VLDL-C), low-density lipoprotein (LDL-C), and
high-density lipoprotein-cholesterol (HDL-C) concentrations were measured using
enzymatic colorimetric assays (Siemens Healthcare Diagnostics INC., Tarrytown,
NY, USA). Serum insulin levels were determined with an electrochemiluminescence
immunoassay (ECLIA) using an immunoassay analyzer (Elecsys 1010/2010 and the E
170 module for Modular Analytics; Roche Diagnostics, Risch-Rotkreuz,
Switzerland). Degree of IR was calculated according to the Homeostasis Model
Assessment based on the following formula: HOMRA-IR = fasting insulin level
(µU/ml) × fasting glucose level
(mmol−1)/22.5.[18 (link)] FSH, LH, progesterone, and 17-ß E2 levels were also measured
with an ECLIA using an immunoassay analyzer (Elecsys 1010/2010 and E 170 module
for Modular Analytics; Roche Diagnostics). Enzyme-linked immunosorbent assay
kits were used to determine SHBG serum concentrations (R&D Systems,
Minneapolis, MN, USA) and visfatin serum concentrations (BioVision Inc.,
Mountain View, CA, USA).
between the 2nd and the 3rd day of her menstrual cycle.
Serum samples were carefully collected and stored at -40°C until
analysis. Glucose and lipid profiles, including the TGs, total cholesterol (TC),
very low-density lipoprotein (VLDL-C), low-density lipoprotein (LDL-C), and
high-density lipoprotein-cholesterol (HDL-C) concentrations were measured using
enzymatic colorimetric assays (Siemens Healthcare Diagnostics INC., Tarrytown,
NY, USA). Serum insulin levels were determined with an electrochemiluminescence
immunoassay (ECLIA) using an immunoassay analyzer (Elecsys 1010/2010 and the E
170 module for Modular Analytics; Roche Diagnostics, Risch-Rotkreuz,
Switzerland). Degree of IR was calculated according to the Homeostasis Model
Assessment based on the following formula: HOMRA-IR = fasting insulin level
(µU/ml) × fasting glucose level
(mmol−1)/22.5.[18 (link)] FSH, LH, progesterone, and 17-ß E2 levels were also measured
with an ECLIA using an immunoassay analyzer (Elecsys 1010/2010 and E 170 module
for Modular Analytics; Roche Diagnostics). Enzyme-linked immunosorbent assay
kits were used to determine SHBG serum concentrations (R&D Systems,
Minneapolis, MN, USA) and visfatin serum concentrations (BioVision Inc.,
Mountain View, CA, USA).