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2 protocols using 5 methyltetrahydrofolate

1

Analytical Standards for Phytochemicals

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Analytical standards (Zeaxanthin, Lutein, β-carotene, Gallic acid, Protocatechuic acid, 4-Hydroxy-benzoic acid, Vanillic acid, Chlorogenic acid, Caffeic acid, p-coumaric acid, Ferulic acid, Sinapic acid, Rutin, Kaempferol, IsoQuercetin, Astragalin, Myricetin, Quercetin, α-tocopherol, γ-tocopherol, δ-tocopherol, 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolic acid, l-ascorbic acid) were obtained from Sigma Aldrich (Poznan, Poland). 10-formylfolic acid and pteroyltri-γ-L-glutamic acid were obtained from Schirck’s Laboratories (Jona, Switzerland). α-amylase obtained from Sigma Aldrich (Poznan, Poland) and γ-glutamyl hydrolase obtained from rat blood plasma (Europa Bioproducts Ltd., Cambridge, UK). Other reagents (acetonitrile, triethylamine, ethyl acetate, pyrogallol, ethanol, potassium hydroxide, orthophosphoric acid, methanol, nitric acid, lanthanum chloride, potassium dihydrogen phosphate) were purchased from Merck (Warsaw, Poland).
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2

Analytical Quantification of Sulfur Metabolism

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Adenosine deaminase, 4-chloro-7-sulfobenzofurazan ammonium salt (SBD-F), L-Cys, cystathionine, Hcy, heptanesulfonate, hydroxocobalamin, L-Met, 5-methyltetrahydrofolate, propargylglycine, pyridoxal 5′-phosphate, quercetin, reduced glutathione (GSH), SAH, SAM, and L-Ser were obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). TRIzol reagent kit and PCR primers for MS, BHMT, CBS, and CSE were purchased from Takara (Dalian, China). FastStart Universal SYBR Green Master mix and cDNA synthesis kit were obtained from Roche (Roche, Switzerland). All other chemicals were of the highest grade available.
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