SFMSCs and SFMSC-iPSC-MSCs were seeded at a density of 5000 cells/cm2 in 6-well plates and incubated in complete culture medium at 37°C in 5% CO2. After 24 h, the medium was replaced with osteogenic induction medium consisting of H-DMEM (Gibco), 10% FBS (Gibco), 10 mM sodium β-glycerophosphate (Santa Cruz Biotechnology), 10 nM 1,25-dihydroxyvitamin D3 (Sigma), and 50 μg/L ascorbic acid-2-phosphate (Wako, Japan). The medium was replaced every 3 days. After induction, medium was assessed by Alizarin Red staining by incubation with a fresh 0.1% Alizarin Red solution for 30 min at 37°C.
Sodium β glycerophosphate
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sodium β-glycerophosphate is a chemical compound used as a buffer and a source of phosphate ions in various laboratory applications. It is a white, crystalline powder that is soluble in water. The compound is commonly used in cell culture media, enzyme assays, and other biochemical procedures where the controlled release of phosphate is required.
Automatically generated - may contain errors
Lab products found in correlation
Ascorbic acid 2 phosphate, by Fujifilm (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
1 25 dihydroxyvitamin d3, by Merck Group (2 mentions)
Dexamethasone (dex), by MP Biomedicals (2 mentions)
H dmem, by Thermo Fisher Scientific (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Alizarin red solution, by Cyagen (1 mentions)
4 protocols using sodium β glycerophosphate
1
Osteogenic Differentiation of SFMSCs
2
Osteogenic Differentiation of SFMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SFMSCs were seeded in 12-well plates at a density of 5,000 cells/cm2. Following adherence, the complete culture medium was replaced with osteogenic induction medium consisting of H-DMEM, 10% fetal bovine serum (FBS) (both from Gibco), 10 mM sodium β-glycerophosphate (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), 10 nM 1,25-dihydroxyvitamin D3 (Sigma-Aldrich, St. Louis, MO, USA) and 50 μg/l ascorbic acid-2-phosphate (Wako, Tokyo, Japan). The culture medium was replaced every 3 days. Following 28 days of induction, osteogenic differentiation was assessed by staining with a fresh 0.1% Alizarin Red solution (Cat. no. 130-22-3; Santa Cruz Biotechnology, Inc.) for 30 min at 37°C.
3
Osteogenic Differentiation of SMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SMSCs were cultured in six-well plates with osteogenic induction medium, which composed of high glucose DMEM (Gibco, USA), 10 mM sodium β-glycerophosphate (Santa Cruz Biotechnology, USA), 10% FBS, 50 μg/L ascorbic acid-2-phosphate (Wako, Japan), and 100 nM dexamethasone (MP Biomedicals, USA). The control group was cultured with complete culture medium. After induction for 2 weeks, the cells in the plates were fixed and stained with alizarin red solution (Cyagen, USA). The gene expression levels of alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX2) were quantitated by reverse-transcription quantitative polymerase chain reaction (qRT-PCR).
4
Osteogenic Differentiation of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded at a density of 5,000 cells/cm2 in 6-well plates and were induced to differentiate the next day. The differentiation medium consisted of high-glucose Dulbecco's modified Eagle's medium (H-DMEM) containing 10% FBS (both Gibco; Thermo Fisher Scientific, Inc.), 10 mM sodium β-glycerophosphate (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), 100 nM dexamethasone (MP Biomedicals, LLC, Santa Ana, CA, USA) and 50 μg/l ascorbic acid-2-phosphate (Wako Pure Chemical Industries, Ltd., Osaka, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!