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Hnrnp 1

Manufactured by Santa Cruz Biotechnology
Sourced in United States

HnRNP I is a protein that plays a role in the regulation of gene expression. It is involved in the processing and transport of mRNA within the cell. HnRNP I is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family of proteins.

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3 protocols using hnrnp 1

1

Quantifying RNA-binding protein expression

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Sources of primary antibodies: c-Myc from Abcam (Cambridge, MA, USA); GAPDH from Protein Tech (Chicago, IL, USA); hnRNP I and AUF1 from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies conjugated with IRDye 800CW or IRDye 680 were purchased from LI-COR Biosciences (Lincoln, NE, USA).
PCR primers were purchased from IDT (Coralville, IA, USA). Linc-RoR and control siRNAs were purchased from ThermoFisher Scientific (Waltham, MA, USA). hnRNP I or AUF1 siRNA mixture was purchased from Santa Cruz Biotechnology. Colon cancer tissue cDNA arrays were purchased from OriGene (Rockville, MD, USA). Breast cancer tissue microarrays (TMAs) were purchased from US Biomax (Rockville, MD, USA).
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2

Antibody and Primer Sources for Cell Research

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Sources of primary antibodies were: p27 and Ki-67 from Cell Signaling (Danvers, MA, USA); hnRNP I from Santa Cruz Biotechnology (Dallas, TX, USA); GAPDH from ProteinTech (Chicago, IL, USA); and β-actin from Sigma-Aldrich (St. Louis, MO, USA). Antibody against phosphorylated hnRNP I were custom made from a synthetic peptide GTKRGSDELF with Ser-16 phosphorylated (PTBP1 amino acids 11–20) by Alpha Diagnostics (San Antonio, TX, USA) using the same procedure as previously described.46 (link) Secondary antibodies conjugated with IRDye 800CW or IRDye 680 were purchased from LI-COR Biosciences (Lincoln, NE, USA).
PCR primers were purchased from IDT (Coralville, IA, USA) (Supplementary Table S1). UCA1 siRNAs and control siRNA were purchased from ThermoFisher Scientific (Waltham, MA, USA). hnRNP I-siRNA mixture was purchased from Santa Cruz Biotechnology. Biotin-labeled UCA1-LNA probe and control oligo for in situ hybridization were purchased from Exiqon (2950 Vedbaek, Denmark). Breast cancer TMAs were purchased from US Biomax (Rockville, MD, USA).
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3

Protein Expression Analysis

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40 μg of whole cell extract was separated by 10% SDS-PAGE and electroblotted to nitrocellulose membrane (Protran, S&S). Immunoblots were incubated with antibodies to C/EBPα (sc-61, Santa Cruz Biotechnology), p27kip1 (sc-528, Santa Cruz Biotechnology), hnRNPI (sc-56701, Santa Cruz Biotechnology) and GAPDH (sc-25778; Santa Cruz Biotechnology).
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