The pulse sequence was implemented on a 14.1T vertical Varian NMR microimaging system (Agilent Technologies, Santa Clara, CA, USA) with a 38mm dual-tuned (13C/1H) volume RF coil. A custom 3D bSSFP sequence was implemented with optimized RF pulses. The sequence can run dynamically and cycle the transmit frequency among the Larmor frequencies of pyruvate, lactate and urea when images are acquired for each metabolite. The RF transmit gain was calibrated with a 13C-enriched urea phantom, placed near the center of the RF coil. Dissolution DNP was performed with a HyperSense polarizer (Oxford Instruments, Oxford, UK) operating at 1.3 K and 3.35 T.
Hypersense
Manufactured by Oxford Instruments
Sourced in United Kingdom, United States, France
HyperSense is a cryogen-free, high-field dynamic nuclear polarization (DNP) system designed for advanced nuclear magnetic resonance (NMR) spectroscopy. It provides a controlled environment for sample preparation and analysis, enabling enhanced sensitivity and resolution in NMR experiments.
Automatically generated - may contain errors
Lab products found in correlation
Dotarem, by Guerbet (8 mentions)
Ox063, by GE Healthcare (4 mentions)
Trityl radical ox063, by Oxford Instruments (3 mentions)
1 13c pyruvic acid, by Merck Group (2 mentions)
2 13c dhac, by Merck Group (2 mentions)
Ox063 trityl radical, by GE Healthcare (2 mentions)
3t mri scanner, by GE Healthcare (1 mentions)
Isotec, by Merck Group (1 mentions)
3t whole body mri scanner, by GE Healthcare (1 mentions)
Ethylenediaminetetraacetic acid disodium salt dehydrate, by Merck Group (1 mentions)
Ox063, by Guerbet (1 mentions)
Gd dota, by Guerbet (1 mentions)
Ge 3t clinical mri, by GE Healthcare (1 mentions)
Gadoterate meglumine, by Guerbet (1 mentions)
1 13c pyruvate, by Cambridge Isotopes (1 mentions)
Oxo63, by GE Healthcare (1 mentions)
Ox063 radical, by GE Healthcare (1 mentions)
1 13c pyruvic acid, by Cambridge Isotopes (1 mentions)
400 mhz nmr, by Agilent Technologies (1 mentions)
Topspin 3, by Bruker (1 mentions)
38 protocols using hypersense
1
Hyperpolarized 13C MRI Pulse Sequence
2
Dissolution-DNP Spin Polarization of [1-13C]Pyruvic Acid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spin polarization and fast dissolution were carried out in a dissolution-DNP spin polarization device (HyperSense, Oxford Instruments Molecular Biotools, Oxford, UK) operating at 3.35 T. Microwave frequency of 94.110 GHz was applied for the polarization of a [1-13C]pyruvic acid formulation at 1.40 to 1.49 K. The formulations consisted of 11.1 to 14.0 mM OX063 radical in the neat acid. The amount of [1-13C]pyruvic acid formulation placed in the polarization cup was 5 ± 0.5 mg in 15 of the experiments described herein and 10 ± 1 mg in 2 experiments. The dissolution medium consisted of 4 ml of 50 mM phosphate buffer which contained 19 mM TRIS and 138.6 mM NaCl. The pH of the dissolution medium was adjusted with NaOH such that upon mixing with the pyruvic acid in the cup the final pH was 7.4.
3
Spin Polarization of Isotopically-Labeled Molecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spin polarization was performed in a dDNP spin polarizer (HyperSense, Oxford Instruments Molecular Biotools) operating at 3.35 T. For [15N2]urea and [13C]urea, the polarization was achieved by irradiating the sample at a microwave (MW) frequency of range of 94.104–94.150 GHz. For [15N]NH4Cl, the polarization was achieved by irradiating the sample at an MW frequency of 94.116 GHz (n = 2) or 94.136 GHz (n = 21). Both polarization protocols were carried out with a power of 100 mW for the MW irradiation, at 1.5 K, for about 2 h. The MW source of the spin polarizer was replaced towards the end of the study (prior to acquiring the data shown in Figure 6 later). To eliminate possible differences in MW source frequency calibrations, the [13C]urea irradiation frequencies were referenced to that of [1‐13C]pyruvic acid P+ (the first maximum in the DNP MW intensity profile), as shown in Figure 3 later.
4
Hyperpolarized Pyruvate Metabolism
5
Hyperpolarized 13C MRI of Diabetic Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The db/db mice and their control littermates db/m mice were fasted for 6 h prior to image acquisition. A tail vein catheter was placed for intravascular access. A 2.2 mol/L solution of [1-13C]DHA in dimethyacetamide containing 15 mmol/L OX063 trityl radical (Oxford Instruments) was HP on a HyperSense dynamic nuclear polarization instrument (Oxford Instruments). The frozen sample was dissolved in distilled water containing 0.3 mmol/L EDTA. Imaging was performed using a 3-T MRI scanner (GE Healthcare, Waukesha, WI) equipped with a multinuclear spectroscopy hardware package. The radiofrequency coil used in these experiments was a dual-tuned 1H-13C coil with a quadrature 13C and 1H channels. Prior to 13C studies, three-plane T2-weighted images were acquired for anatomic localization (echo time 100 ms; repetition time 4 s; six averages) using a standard fast spin echo sequence. 13C MR spectroscopic imaging studies were then acquired 25 s postinjection of 15 mmol/L HP 13C DHA, at 5-mm isotropic resolution, as previously published (21 (link),24 (link)).
6
Hyperpolarized [1-13C]Pyruvate Injection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the HP process, 14 M [1-13C]pyruvate (36.7 ± 1.9 mg) supplemented with 15 mM OX063 trityl radical and 1 mM Dotarem were polarized with a HyperSense (Oxford Instruments, Abingdon, United Kingdom) for ~30 min at 1.2 K using a microwave frequency of 94.155 GHz and 100 mW power. The sample was dissolved in a solution pressurized to 10 bar and heated to 180 °C containing 4.5 ± 0.3 mL 100 mM phosphate buffered saline (PBS) supplemented with 100 mM sodium hydroxide (NaOH) and 0.1 g/L sodium ethylenediaminetetraacetic acid (EDTA). Finally, the solution for injection contained an average pyruvate concentration of 90.3 ± 3.9 mM at pH 7.6 ± 0.2. The polarization level was about 38% (measured at B0 = 1 T, Spinsolve Carbon, Magritek, Aachen, Germany) 38 . The hyperpolarized solution was rapidly transferred to the clinical PET/MR scanner and injected 21.7 ± 2.1 s after dissolution.
7
Hyperpolarized 13C-Pyruvate Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A solution of 20 μL of 13C pyruvic acid (ISOTEC; Sigma-Aldrich), 15 mM OX63 trityl radical (GE Healthcare), and 1.5 mM gadolinium chelate (ProHance) was polarized at 3.35 T and 1.4 K using dynamic nuclear polarization (HyperSense; Oxford Instruments) for one hour. A frozen 13C-pyruvate sample was rapidly dissolved in 4 mL of superheated alkaline buffer containing 100 mg/L EDTA, 40 mM NaOH, 40 mM TRIS buffer, and 30 mM NaCl. The final concentration of pyruvate injected into the mouse tail vein was 80 mM, with a physiologic pH of about 7.4. Next, 200 μL of hyperpolarized pyruvate was injected into mice using a tail vein catheter for 8–10 seconds.
8
Hyperpolarized Probe 3 for DNP-NMR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 4.4 M solution of probe 3 containing 23% glycerol and 19 mM OX063 (GE Healthcare, Chicago, IL) was prepared (pH adjusted to 7 with 10 M HCl aq.). The sample was submerged in liquid helium in a DNP polarizer magnet (3.35 T; HyperSense, Oxford Instruments, Oxford, UK). Hyperpolarization was achieved using microwave irradiation at 94 GHz and 100 mW for approximately 1.5 hours under 2.8 mbar at 1.45 K. Then, hyperpolarized samples were dissolved in DPBS containing 0.68 mM EDTA. DNP-NMR measurements were performed using a 3-T MR Solutions animal scanner (MR Solutions Ltd., Guildford, UK) in the absence or presence of 250 nM rat APN (164599; Merck, Darmstadt, Germany). The apparent T1 was obtained by curve fitting to signal decay profile, and the corrected T1 was estimated by considering the magnetization loss due to successive flip angles. The details are described in the Supplementary Materials.
9
Hyperpolarized 13C Liver Imaging in Rats
10
Hyperpolarization of 13C Bicarbonate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
13C cesium bicarbonate was hyperpolarized using dynamic nuclear polarization (HyperSense, Oxford Instruments, Abingdon, UK). The bicarbonate sample was cooled to 1.4 K at 3.3 T in the dynamic nuclear polarization apparatus and irradiated with 50 mW of microwave radiation at a frequency of 94.123 GHz to transfer electronic spin magnetization to the 13C-labeled bicarbonate. Approximately 90% solid-state polarization was achieved during 2 h of hyperpolarization. The sample was rapidly mixed with a superheated mixture of 80-mmol/L phosphate buffer (pH = 7.2) and 100-mg/L ethylenediamine tetraacetic acid disodium salt dehydrate (Sigma-Aldrich, St. Louis MO). The resulting hyperpolarized solution had a 13C bicarbonate concentration of 150 mmol/L and a pH of 7.4 at 37°C. The in vitro spin lattice relaxation time and polarization values of the 13C nucleus were 26.2 ± 1.4 s and ∼7.5% measured at 3 T (24 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!