The effect of different concentrations of N source was determined at 11.89, 27.84, 36.20 and 44.55 (g/L) of yeast extract (Ohly KAT, Germany). The concentration (g/L) of glucose was fixed at 20.0, sodium acetate at 5.0, diammonium hydrogen citrate at 2.0, dipotassium hydrogen phosphate at 2.0, Tween 80 at 1.0, magnesium sulphate heptahydrate at 0.2 and manganese sulphate tetrahydrate at 0.04, which followed the composition of MRS broth (Table 8 ; Merck, Darmstadt, Germany). glucose, sodium acetate, diammonium hydrogen citrate, dipotassium hydrogen phosphate, Tween 80, magnesium sulphate heptahydrate and manganese sulphate tetrahydrate were purchased from Merck Company (Darmstadt, Germany). The experiment was performed in triplicate with 2 h sampling intervals.
Dipotassium hydrogen phosphate
Manufactured by Merck Group
Sourced in Germany, United States, India
Dipotassium hydrogen phosphate is a chemical compound with the formula K2HPO4. It is a colorless, crystalline solid that is soluble in water. Dipotassium hydrogen phosphate is commonly used as a buffer in various laboratory and industrial applications to maintain a specific pH level.
Automatically generated - may contain errors
Lab products found in correlation
Potassium dihydrogen phosphate, by Merck Group (75 mentions)
Sodium hydroxide (naoh), by Merck Group (29 mentions)
Hydrochloric acid (hcl), by Merck Group (27 mentions)
Sodium chloride (nacl), by Merck Group (25 mentions)
Hydrogen peroxide, by Merck Group (15 mentions)
Potassium chloride (kcl), by Merck Group (14 mentions)
Methanol, by Merck Group (14 mentions)
Gallic acid, by Merck Group (12 mentions)
Ascorbic acid, by Merck Group (11 mentions)
Trichloroacetic acid, by Merck Group (11 mentions)
Sodium carbonate, by Merck Group (11 mentions)
Potassium hydroxide, by Merck Group (10 mentions)
Quercetin, by Merck Group (10 mentions)
Ethanol, by Merck Group (9 mentions)
Disodium hydrogen phosphate, by Merck Group (9 mentions)
Folin ciocalteu reagent, by Merck Group (9 mentions)
Tween 20, by Merck Group (8 mentions)
Potassium ferricyanide, by Merck Group (8 mentions)
Ferric chloride, by Merck Group (7 mentions)
Sodium dihydrogen phosphate, by Merck Group (7 mentions)
121 protocols using dipotassium hydrogen phosphate
1
Optimizing Nitrogen Source Concentration for Microbial Growth
2
Antioxidant Compound Extraction and Analysis
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Bismuth(III) nitrate, l-(+)-tartaric acid, potassium iodide, mercury(II) chloride, sodium acetate, isoamyl alcohol, ferric chloride, magnesium chips, isoamyl alcohol, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium carbonate, butylated hydroxytoluene, butylated hydroxyanisole, gallic acid and quercetin were used as standards, aluminum chloride (AlCl3), DPPH, Folin-Ciocalteu's phenol reagent and trichloroacetic acid (TCA) were acquired from Sigma-Aldrich. For acetic anhydride, sulfuric acid, formaldehyde, hydrochloric acid, and solvents used were of analytical grade were purchased from Merck Co. (Darmstadt, Germany).
3
Antioxidant Enzyme Activity Assay
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Methanol, Trichloroacetic acid (TCA), 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB), Hydrogen Peroxide (H2O2), potassium dihydrogen phosphate, pyragallol, potassium hydroxide, di-potassium hydrogen phosphate were purchased from Sigma-Aldrich (USA). Ketamine was obtained from Caraway pharmaceutical (Pakistan). Sinemet was obtained from OBS (Pakistan). All the chemicals used were of analytical grade.
4
HPLC Analysis of Pharmaceutical Compounds
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For HPLC analysis, chromatographic-grade methanol was used (VWR, Vienna, Austria). A Millipore purifier (Millipore, Burlington, MA, USA) was used to obtain water for HPLC. Potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid, potassium chloride, quercetin, hispiduline, ascorbic acid, ibuprofen, ketoprofen, sodium chloride, hydrogen peroxide, trypsin, Tris-HCl buffer, sodium nitroprusside, sodium salicylate, ferrous sulfate, sulfanilamide, naphthylethylenediamine dihydrochloride and perchloric acid were purchased from Sigma-Aldrich, Taufkirchen, Germany. Human albumin 20%—BB, 200 g/L was ordered from BB-NCIPD Ltd., Sofia, Bulgaria.
5
Synthesis and Characterization of Gold Nanoparticles
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Gold nanoparticles, 60 and 80 nm diameter, OD = 1 in PBS buffers were obtained from Sigma-Aldrich. Urea, lactic and citric acids, sodium bicarbonate, calcium chloride, sodium chloride, sodium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium chloride were also all procured from Sigma-Aldrich. Paracetamol was purchased from a drugstore, and was ground into powder before dissolution in deionized (DI) water Al foil was purchased in a supermarket. DI water was obtained from Milli-Q system. Gold film on glass microscope slides of 100 nm thickness and 99.9% purity, prepared by evaporation under vacuum, were purchased from EMF Corporation (USA).
6
Antioxidant and Anti-inflammatory Assay Protocol
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2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS); bovine
catalase; bovine serum albumin (BSA); butylated hydroxyl toluene (BHT); eosin;
epinephrine; Folin-Ciocalteu; hydrochloric acid (HCl); hematoxylin; hydrogen
peroxide (H2O2); mEthanol; paraffin; potassium dihydrogen
phosphate (KH2PO4); dipotassium hydrogen phosphate (K2HPO4); rutin; sodium
hydroxide (NaOH); sodium pentobarbital (C11H18N2O3); tannic acid;
2-thio-barbituric acid (TBA) and trichloroacetic acid (TCA) were purchased
products from Sigma Chemical Co. (Sigma-Aldrich GmbH, Steinheim, Germany).
Ethanol (EtOH); sulfasalazine; sodium chloride (NaCl); loperamide; and castor
oil were supplied by the Central Pharmacy of Tunisia. All other reagents used
were of analytical quality.
catalase; bovine serum albumin (BSA); butylated hydroxyl toluene (BHT); eosin;
epinephrine; Folin-Ciocalteu; hydrochloric acid (HCl); hematoxylin; hydrogen
peroxide (H2O2); mEthanol; paraffin; potassium dihydrogen
phosphate (KH2PO4); dipotassium hydrogen phosphate (K2HPO4); rutin; sodium
hydroxide (NaOH); sodium pentobarbital (C11H18N2O3); tannic acid;
2-thio-barbituric acid (TBA) and trichloroacetic acid (TCA) were purchased
products from Sigma Chemical Co. (Sigma-Aldrich GmbH, Steinheim, Germany).
Ethanol (EtOH); sulfasalazine; sodium chloride (NaCl); loperamide; and castor
oil were supplied by the Central Pharmacy of Tunisia. All other reagents used
were of analytical quality.
7
Sunflower Oil Characterization Protocol
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Commercial sunflower oil was purchased from a local market. Sesamol (> 98%), Tween 80, ammonium thiocyanate, potassium dihydrogen phosphate, di-potassium hydrogen phosphate, PGPR, and barium chloride were purchased from Sigma-Aldrich Company (St. Louis, MO). Chloroform, n-heptane, hydrochloric acid, and methanol were purchased from Merck Company (Darmstadt, Germany).
8
Surface Functionalization for Protein Assays
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Absolute ethanol 99.8%, bovine serum albumine (BSA), dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium hydroxide, dithioerythritol (DTE), dithiothreitol (DTT), 3-glycidyloxypropyl trimethoxysilane (GOPTS), guanidinium hydrochloride (GuHCl), hydrogen chloride (37%), methanol, Pluronic® F-127, poly(ethylene glycol) diglycidyl ether (diepoxy-PEG, MN = 500), potassium dihydrogen phosphate, sodium azide, sodium carbonate, sodium chloride, sodium dodecyl sulfate (SDS), sodium hydrogen carbonate, fuming sulfuric acid, D-(+)-trehalose dihydrate, tris(hydroxymethyl)aminomethane Sigma 7–9® (TRIS), Tween®-20 and urea were obtained from Sigma-Aldrich (Taufkirchen, Germany). 3-(N-morpholino)-propanesulfonic acid (MOPS PUFFERAN®) and Menzel glass slides (76 mm × 26 mm × 1 mm) were obtained from Carl Roth (Karlsruhe, Germany). Hellmanex was obtained from Hellma GmbH (Mannheim, Germany). Jeffamine® ED-2003 polyetheramine (DAPEG) was obtained from Huntsman Belgium BVBA (Everberg, Belgium) as a gift. WESTAR supernova ELISA luminol and hydrogen peroxide were purchased from Cyanagen (Bologna, Italy). The ARcare 90106 adhesive film was obtained from Adhesive Research Ireland (Limerick, Ireland). The poly(methyl methacrylate) support for the chip was produced in our workshop. 384-well PP flat bottom microtiter plates (MTPs) were obtained from Greiner GmbH (Frickenhausen, Germany).
9
In vitro MAO Inhibition Assay
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Harmine was obtained from THC Pharm (Frankfurt, Germany), amphetamine-d 5 and AMT succinate from LGC Standards (Wesel, Germany), harmaline, yohimbine, selegiline, kynuramine (KYN), 4-hydroxyquinoline (4-OHC), ammonium acetate, potassium dihydrogenphosphate, and dipotassium hydrogenphosphate from Sigma-Aldrich (Taufkirchen, Germany), formic acid (MS grade) from Fluka (Neu-Ulm, Germany), acetonitrile, methanol (both LC-MS grade), and all other chemicals from VWR (Darmstadt, Germany). 5-IT was synthesized (Scott et al. 2014 ) and kindly provided by the Department of Pharmacology and Therapeutics, Trinity Centre for Health Sciences, St James's Hospital, Dublin 8, Ireland, before it was scheduled. All non-scheduled AMT analogs were prepared following procedures published previously (Young 1958) . Details can also be found in (Brandt et al. 2004 ).
The baculovirus-infected insect cell microsomes (Supersomes) containing human complementary DNA-expressed MAO-A or MAO-B (5 mg protein/mL), wildtype Supersomes as negative control without MAO activity (MAO control, 5 mg protein/mL), and pooled human liver S9 (20 mg protein/mL) were obtained from Corning (Amsterdam, The Netherlands). After delivery, enzyme preparations were thawed at 37 °C, aliquoted, snap-frozen in liquid nitrogen, and stored at -80 °C until use.
The baculovirus-infected insect cell microsomes (Supersomes) containing human complementary DNA-expressed MAO-A or MAO-B (5 mg protein/mL), wildtype Supersomes as negative control without MAO activity (MAO control, 5 mg protein/mL), and pooled human liver S9 (20 mg protein/mL) were obtained from Corning (Amsterdam, The Netherlands). After delivery, enzyme preparations were thawed at 37 °C, aliquoted, snap-frozen in liquid nitrogen, and stored at -80 °C until use.
10
Quantification of Amino Acids and Sugars
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Chemicals and solvents were of HPLC or analytical grade. A certified reference material, the solution containing 17 amino acids in 0.1 M HCl and the solid standard amino acids and sugars β-alanine, L-citrulline, L-ornitihine monohydrochloride, taurine, gamma-aminobutyric acid (GABA), D-fructose, xylitol and tartaric acid were acquired from Sigma Alrich (St. Louis, Missouri, USA). L-asparagine monohydrate, L-glutamine, L-tryptophan and L-theanine were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), D-glucose and sucrose from VWR Chemicals (BDH Prolabo, Leuven Belgium) and Tri-Sil HTP Reagent was obtained from Thermo Scientific. Methanol (MeOH), acetonitrile (ACN), hydrochloric acid (35%), potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium phosphate monobasic and 3-mercaptopropionic acid (3-MPA) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and VWR Chemicals (Radnor, Pennsylvania, USA). Boric acid was purchased from Merck KGaA (Darmstadt, Germany) and sodium hydroxide from VWR Chemicals (BDH Prolabo, Leuven Belgium). Derivatization reagent o-phthalaldehyde (OPA, ≥ 98%) and 85% ortophosphoric acid (85-90%) were acquired from MP Biomedicals (Santa Ana, California, USA). Ethanol anhydride was acquired from Yliopiston Apteekki (Helsinki, Finland).
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