Pycr1

MCF-7 cells (ATCC^® HTB-22™) were obtained from ATCC (Manassas, VA, USA). MEM Eagle’s medium, fetal bovine serum, puromycin, Pen/Strep mix, and PBS were products of Gibco (Waltham, MA, USA). Propidium iodide (PI), annexin V-CF488A conjugate, and annexin V binding buffer were provided by the Biotium Company (Fremont, CA, USA). NC-Slide A2™ was purchased from Chemometec (Allerod, Denmark). Horseradish peroxidase conjugated anti-rabbit IgG and anti-mouse IgG antibodies, bacterial collagenase, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescin diacetate (DCFDA), indomethacin, and diclofenac were purchased from Sigma Aldrich (Saint Louis, MO, USA). Primary antibodies against COX2, p-AMPKα, mTor, caspase 8, caspase 9 total and cleaved, caspase 7 total and cleaved, BID, PARP, Beclin 1, AGT5, ATG 7, and B-actin were products of Cell Signaling Technology (Danvers, MA, USA). Primary antibodies for PRODH/POX, PPARγ, GLUD 1/2, and prolidase were products of Santa Cruz Biotechnology (Dallas, TX, USA). PYCR1, PYCRL, and PPARδ primary antibodies were obtained from Abnova (Taipei, Taiwan). Hoechst 33342 was obtained from Becton Dickinson (Franklin Lakes, NJ, USA). CRISPR All-In-One Non-Viral Vector and lipofectamine were products of Applied Biological Materials Inc. (Richmond, BC, Canada).

MCF7 (ATCC^® HTB-22™) were obtained from the ATCC. Horseradish-peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies, Alexa488 conjugated anti-rabbit IgG, bacterial collagenase, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DCFDA, tetrahydro-2-furoic acid (THFA), glycyl-L-proline, ibuprofen, indomethacin, diclofenac, and sulindac were purchased from Sigma-Aldrich. The celecoxib and troglitazone were products of Targetmol. Solution 5 (VB-48™ PI–AO) was purchased from ChemoMetec. The primary antibodies against: COX2; p53; p-AMPKα; m-TOR; caspase-8; caspase-9, total and cleaved; caspase-7, total and cleaved; PARP, total and cleaved; and B-actin were products of Cell Signaling Technology. The PRODH/POX primary antibodies and PPARγ were products of Santa Cruz Biotechnology. The PYCR1, PYCRL, and PPAR delta primary antibodies were obtained from Abnova. The Hoechst 33342 was obtained from Becton Dickinson.