25HC was from Sigma Aldrich (St. Louis, MO) and ent-25HC was custom-synthesized by Avanti Polar Lipids (Birmingham, AL). The GPR183-specific antagonist NIBR189 was from Tocris (Bio-Techne Corp, Minneapolis, MN).
Nibr189
Manufactured by Bio-Techne
NIBR189 is a laboratory equipment product from Bio-Techne. It is a device used for analysis and measurement purposes in various research and scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nibr189
1
Agonist and Antagonist Effects on GPR183
2
Assay for Calcium Signaling in Differentiated BAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Differentiated BAs were seeded on a black 96-well plate with clear bottom. The following day, the assay was performed according to manufacturer’s instruction in presence of 7α,25-OHC (1 μM, Avanti Biolipids), NIBR189 (10 μM, Tocris), Norepinephrine (1 μM, Sigma-Aldrich) or H2O2 (3%, Sigma-Aldrich). The fluorescence increase was measured at different time points at Ex/Em = 520/605 nm at bottom read mode with a EnSpire™ Multimode Plate Reader (Perkin Elmer).
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