Axio observer z1 microscope

Manufactured by Zeiss

Sourced in Germany, United States, United Kingdom, France, Japan, Canada, Denmark, Italy, Austria

The Axio Observer Z1 is an inverted research microscope designed for advanced imaging applications. It features a modular and flexible platform that can be configured with a variety of accessories and imaging modules to meet the specific needs of researchers and scientists. The core function of the Axio Observer Z1 is to provide high-quality optical performance and advanced imaging capabilities for a wide range of microscopy techniques, including fluorescence, brightfield, phase contrast, and differential interference contrast (DIC).

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Close spelling variants of this product

Axio Observer Z1 inverted microscope Axio Observer Z1 epifluorescent microscope Axio Observer Z1 motorized FL inverted microscope Axio Observer.Z1 inverted epifluorescence microscope Axio Observer Z1 Inverted Microscope with Apotome Axio Observer Z1 optical microscope Axio Observer Z1 inverted fluorescence microscope Axio Observer.Z1 invert confocal microscope Axio Observer Z1 Microscope System LSM Axio Observer Z1 confocal microscope

1 025 protocols using axio observer z1 microscope

Imaging and Nuclear Translocation Assays

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For imaging, young adult worms were paralyzed by 2,3-butanedione monoxime (BDM), mounted on 2% agarose pads, and imaged using a Zeiss Axio Observer Z1 microscope.
For nuclear translocation scoring, animals were analyzed on day 2 of adulthood after exposure to the following conditions: for low IIS, daf-2(e1370) background worms were shifted to 25 °C for 6 h to inactivate IIS; for lack of a germline, glp-1(e2141) background animals were raised at 25 °C from the egg stage to prevent germline proliferation; for heat stress, animals were exposed for 3 h to 35 °C; for oxidative stress, animals were exposed for 3 h to 100 mM tert-butylhydroperoxide (tBOOH); for UV irradiation, animals were exposed to 360 mJ cm⁻² followed by 45 min of recovery; for starvation, animals were transferred for 6 h to plates without food; for pathogen exposure, animals were grown for 1 day on Pseudomonas araginosa PA14. Worms were mounted on 2% agarose pads in the presence of BDM, and the DAF-16::GFP or HLH-30::GFP nuclear translocation in the intestine was scored immediately, using a Zeiss Axio Observer Z1 microscope. To avoid subtle translocation phenotypes caused by exposure to the mounting and imaging conditions, all scoring was conducted within 5 min after mounting.

Lin X.X., Sen I., Janssens G.E., Zhou X., Fonslow B.R., Edgar D., Stroustrup N., Swoboda P., Yates JR 3.r.d., Ruvkun G, & Riedel C.G. (2018). DAF-16/FOXO and HLH-30/TFEB function as combinatorial transcription factors to promote stress resistance and longevity. Nature Communications, 9, 4400.

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GFP Fluorescence Imaging in Cell Cultures

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GFP expression in cell cultures at various time points was examined using Zeiss AxioObserver Z.1 microscope equipped with AxioCam MRc digital camera and appropriate filters. Exposure times were adjusted for optimum imaging and kept consistent for each time point of the culture. Panoramic images of larger areas of the cultures were obtained using a computer-controlled motorized imaging workstation and Zeiss AxioObserver Z.1 microscope.

Sagomonyants K, & Mina M. (2015). Stage-specific effects of FGF2 on the differentiation of dental pulp cells. Cells, tissues, organs, 199(0), 311-328.

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Visualizing TβRII Localization in Cells

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A549 and Mv1Lu cells were cultured on 24-mm round coverslips. For transient transfection, cells were transfected with TβRII-Flag using the Lipofectamine 3000 reagent as per the manufacturer's instructions. After 24 hours of transfection, cells were treated with PG for 4 hours at 37°C. Following treatment, the cells were fixed in 4% paraformaldehyde and then permeabilized with PBS containing 0.2% Triton for 20 minutes. Subsequently, fixed cells were washed and blocked with 5% BSA PBS for 1 hour at room temperature (RT), followed by overnight incubation with the appropriate primary antibody solution at 4°C. After washing, cells were incubated with Alexa Fluor-conjugated secondary antibodies for 1 hour at RT. The coverslips were mounted using mounting medium, with DAPI used as a nuclear counterstain. For visualization of stress fibers, cells were fixed and permeabilized following the aforementioned protocol and were stained with Alexa Fluor 594-conjugated phalloidin (ThermoFisher #A12 381) to label F-actin. The samples were examined using a Zeiss AxioObserver Z1 microscope (Oberkochen, Germany), and images were acquired using AxioVision Rev 4.6 software. To determine the localization of TβRII, we utilized the Zeiss AxioObserver Z1 microscope equipped with Apotome optical sectioning technique to acquire single focal plane images.

Tai S.B., Huang C.Y., Chung C.L., Sung P.J., Wen Z.H, & Chen C.L. (2024). Prodigiosin Inhibits Transforming Growth Factor β Signaling by Interfering Receptor Recycling and Subcellular Translocation in Epithelial Cells. Molecular pharmacology, 105(4).

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Membrane Staining and Sporulation Visualization

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For membrane staining, Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark. Hyphae were then directly spotted onto a 1% agarose pad and visualized using a Zeiss Axio Observer Z1 microscope using an Alpha Plan-Apo 100×/1.46 Oil DIC M27 objective. Spores of SV55 were loaded into a BA04 microfluidic plate (ONIX, CellASIC) and allowed to germinate and to grow using constant perfusion of MYM containing 5.5 μg/ml FM4-64 at 30 °C. To promote sporulation, MYM/FM4-64 medium was replaced after 3 h of growth by “spent-MYM”/FM4-64. The “spent-MYM” was prepared as described previously²⁹. Hyphae were visualized using a Zeiss Axio Observer Z1 microscope equipped with a Plan Apochromat 100×/1.4 Oil Ph3 objective. Images were collected and analyzed using Zen Blue (Zeiss) or Fiji²⁸. We note that FM4-64 staining using the ONIX microfluidics systems is inefficient as the membrane dye appears to bind to the internal plate material. Thus, the majority of images shown in the manuscript were generated using cells immobilized on agarose pads.

Bush M.J., Gallagher K.A., Chandra G., Findlay K.C, & Schlimpert S. (2022). Hyphal compartmentalization and sporulation in Streptomyces require the conserved cell division protein SepX. Nature Communications, 13, 71.

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Dexmedetomidine Effects on Neuronal Cytoskeleton

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Immunofluorescent staining was performed to assess the impact of dexmedetomidine on neuronal cytoskeletal growth in neurons grown for 7 days post-culture. Fixation and staining were done as previously described for neurofilament antibody^{34 (link)}. Cells were imaged on a Zeiss Axio Observer Z1 microscope (Zeiss Corp.) using a 20×/0.8 DICII objective. Fifteen to twenty neurons per biological replicate (n = 12–13) were quantified.
A procedure similar to the one previously outlined was followed to evaluate the impact of DEX on synaptic network assembly in neurons grown for 7 days post-culture using synaptophysin and PSD-95 antibodies as previously described^{34 (link)}. Cells were imaged on a Zeiss Axio Observer Z1 microscope (Zeiss Corp.) using a 63×/1.4 oil DICII objective 15–20 neurons per biological replicate (n = 8–10) were quantified.

Jimenez-Tellez N., Iqbal F., Pehar M., Casas-Ortiz A., Rice T, & Syed N.I. (2021). Dexmedetomidine does not compromise neuronal viability, synaptic connectivity, learning and memory in a rodent model. Scientific Reports, 11, 16153.

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Immunofluorescence and Apoptosis Analysis

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Cell samples were fixed in 10% formalin washed in Hank's solution, and blocked using PBS supplemented with 10% goat normal serum. Samples were then washed with PBST and then treated with primary antibodies at 4°C overnight. Subsequently, samples were then incubated with fluorescence-conjugated secondary antibodies. After stained with DAPI, areas of interest were captured by a Zeiss Axio Observer Z1 microscope.
Apoptosis was assessed by propidium iodide (PI) staining using a PI staining Kit (P1304MP, ThermoFisher). Images were acquired using a Zeiss Axio Observer Z1 microscope.

Cai C., Li Z., Zheng Z., Guo Z., Li Q., Deng S., Shi N., Ou Q., Zhou H., Guo Z., Chen Z, & Zhu H. (2023). Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response. International Journal of Biological Sciences, 19(14), 4657-4671.

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Dietary Lipids Modulate Retinal Neovascular Lesions in AMD Mouse Model

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Vldlr⁻^/⁻ mice develop pathological RAP similar to AMD.^{25 (link)} Neovessels extend from the deep retinal vascular layer of the outer plexiform layer (OPL) toward the retinal pigment epithelium (RPE). Mice were fed defined rodent diets with either 2% ω-3 (DHA and EPA) or 2% ω-6 LCPUFA (AA)^{23 (link)} from postnatal day (P) 1. At P16, the eyes were enucleated and fixed in 4% PFA for 1 hour at room temperature. The retinas were dissected and stained overnight with fluorescent Griffonia Bandeiraea Simplicifolia Isolectin B4 (Alexa Fluor 594, I21413, Molecular Probes, 10 μg/mL) in 1 mM CaCl₂ in PBS, then whole mounted with photoreceptors facing up. Images were taken at 50× magnification on a Zeiss AxioObserver.Z1 microscope and merged to form one image with AxioVision 4.6.3.0 software. Vascular lesions were analyzed using the SWIFT_MACTEL method, a plugin in ImageJ.^{25 (link)}

Fu Z., Liegl R., Wang Z., Gong Y., Liu C.H., Sun Y., Cakir B., Burnim S.B., Meng S.S., Löfqvist C., SanGiovanni J.P., Hellström A, & Smith L.E. (2017). Adiponectin Mediates Dietary Omega-3 Long-Chain Polyunsaturated Fatty Acid Protection Against Choroidal Neovascularization in Mice. Investigative Ophthalmology & Visual Science, 58(10), 3862-3870.

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Seam Cell Fusion Analysis in C. elegans

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All strains were grown on OP50-seeded NGM plates supplemented with 1 mM auxin. Gravid animals were bleached and eggs were incubated at room temperature in M9 for hatching overnight. To allow accumulation of LIN-29a, LIN-29b or MAB-10, the animals were not exposed to auxin anymore from this point onward. Synchronized L1 animals were plated and grown at 25°C. Seam cell fusion was scored by observation of the ajm-1::mCherry marker at 15 hr after plating synchronized L1 animals. Fluorescent and Differential Interference Contrast (DIC) images were acquired with a 40x/2.5 oil immersion objective with a Zeiss Axio Observer Z1 microscope using the AxioVision SE64 software and Zen 2 (blue edition). Selections of regions and processing of images was performed with Fiji (Schindelin et al., 2012 (link)).

Azzi C., Aeschimann F., Neagu A, & Großhans H. (2020). A branched heterochronic pathway directs juvenile-to-adult transition through two LIN-29 isoforms. eLife, 9, e53387.

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Fluorescent Microscopy of Cervical Tissue

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Images of the H&E stained cervical tissue sections were acquired using a Zeiss Axio Observer Z1 microscope and an AxioVision 4.6.3-AP1 camera using brightfield and at an excitation wavelength of 450–490 nm with emission captured at 515–565 nm (Fig. 1). The 32-bit grayscale fluorescent images were used for image analysis (see below). The same exposure time was used across all samples.

Castellanos M.R., Szerszen A., Gundry S., Pirog E.C., Maiman M., Rajupet S., Gomez J.P., Davidov A., Debata P.R., Banerjee P, & Fata J.E. (2015). Diagnostic imaging of cervical intraepithelial neoplasia based on hematoxylin and eosin fluorescence. Diagnostic Pathology, 10, 119.

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Fluorescent Tagging of dpf-3 and pgl-1

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All confocal images were acquired exactly as described in (Aeschimann et al., 2017) . Fluorescent and Differential Interference Contrast (DIC) images represented in Fig. XXX were obtained with Zeiss Axio Observer Z1 microscope and AxioVision SE64 software and Zen 2 (blue edition). Fiji was used for selecting/cropping and processing of the images. Construction of Fluorophore tagged dpf-3 and pgl-1
Using the plasmid pIK377 as a template that contains 3xFLAG::TEV::GFP::HiBit, PCR was done with DPF-3 overhang fw-oIK1315 and DPF-3 overhang rev-oIK1288 to generate final asymmetric PCR product containing the intended tag and homology (120 nucleotides) to dpf-3 gene and tagged lines were obtained as described in (Dokshin et al., 2018) . The tag in the dpf-3 is inserted just before the translation stop codon. The RFP tagged pgl-1 strain is a kind gift from Ketting laboratory and is described in the submitted manuscript (Schreier et al, unpublished) .

Gudipati R.K., Braun K., Gypas F., Heß D., Schreier J., Carl S.H., Ketting R.F., & Großhans H. (2020). Protease-mediated processing of Argonaute proteins controls small RNA association.

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