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Quantikine human immunoassay kit

Manufactured by R&D Systems
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The Quantikine Human Immunoassay kits are in vitro diagnostic products designed to quantitatively measure specific human proteins in cell culture supernates, serum, plasma, and other biological fluids. The kits utilize enzyme-linked immunosorbent assay (ELISA) technology.

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Lab products found in correlation

No assay kit, by Thermo Fisher Scientific (2 mentions) Duoset elisa development systems kit, by R&D Systems (1 mentions) Chemiluminescence immunoassay, by Siemens (1 mentions) 2030 arvo x3, by PerkinElmer (1 mentions) Gag assay kit, by Biocolor (1 mentions)

Close spelling variants of this product

Quantikine ELISA Human Amyloid β aa1-40/aa1-42 immunoassay kits Human Quantikine® Immunoassay kits Quantikine Human Immunoassay Quantikine Immunoassay kits Quantikine Immunoassays Quantikine Human Quantikine kits Quantikines Human kits

13 protocols using quantikine human immunoassay kit

1

Growth Factor Quantification in Cell Culture

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During the cell culture period, the spent medium was collected for the analysis of growth factors; transforming growth factor (TGF)-β and interleukin (IL)-1β were chosen for the analyses. Active TGF-β and IL-1β concentrations were determined using enzyme-linked immunosorbent assay (ELISA) in duplicate aliquots of all samples with the Quantikine Human Immunoassay kits (R&D Systems, Minneapolis, Minnesota).
Chiu C.H., Chen P., Yeh W.L., Chen A.C., Chan Y.S., Hsu K.Y, & Lei K.F. (2019). The gelling effect of platelet-rich fibrin matrix when exposed to human tenocytes from the rotator cuff in small-diameter culture wells and the design of a co-culture device to overcome this phenomenon. Bone & Joint Research, 8(5), 216-223.
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2

Quantification of PGE2 and NO

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The amount of PGE2 released into the medium was quantified by ELISA using the Quantikine Human Immunoassay kits (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions. The production of NO was measured by Griess reaction using the NO Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) according to manufacturer’s instructions.
Yin W., Qi X., Zhang Y., Sheng J., Xu Z., Tao S., Xie X., Li X, & Zhang C. (2016). Advantages of pure platelet-rich plasma compared with leukocyte- and platelet-rich plasma in promoting repair of bone defects. Journal of Translational Medicine, 14, 73.
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3

Quantifying Angiogenic Factors in Co-cultures

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The concentration of VEGF-A, TGF-β and PDGF-BB released in single cultures and in co-culture supernatants were determined by ELISA using Quantikine Human Immunoassay kits (all by R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s protocol. After 48 hrs of co-culture and tube formation, media samples were collected and tested.
Cipriani P., Di Benedetto P., Ruscitti P., Campese A.F., Liakouli V., Carubbi F., Pantano I., Berardicurt O., Screpanti I, & Giacomelli R. (2014). Impaired Endothelium-Mesenchymal Stem Cells Cross-talk in Systemic Sclerosis: a Link Between Vascular and Fibrotic Features. Arthritis Research & Therapy, 16(5), 442.
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4

Activation and Cytokine Release of PRP

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PCP obtained from different first-spin conditions and P-PRP obtained using different second-spin conditions were activated with 10% CaCl2 (final concentration, 22.8 mM). Subsequently, the formulations were incubated at 37°C in a humidified atmosphere with 5% CO2 for 2 h. At the end of the incubation period, the formulations were centrifuged at 2,800×15 and the supernatant was collected and stored at −80°C until analysis. The supernatant was assayed for IL-1β, tumor necrosis factor (TNF)-α, platelet-derived growth factor (PDGF)-AB and transforming growth factor (TGF)-β1 with Quantikine Human Immunoassay kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol.
Yin W., Xu H., Sheng J., Zhu Z., Jin D., Hsu P., Xie X, & Zhang C. (2017). Optimization of pure platelet-rich plasma preparation: A comparative study of pure platelet-rich plasma obtained using different centrifugal conditions in a single-donor model. Experimental and Therapeutic Medicine, 14(3), 2060-2070.
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5

Quantification of Growth Factors and Cytokines

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PDGF-AB, TGF-β1, VEGF, IL-1β, and TNF-α concentrations of whole blood, L-PRP, and P-PRP were quantified by enzyme-linked immunosorbent assay (ELISA) [30 (link)]. In brief, whole blood, L-PRP, and P-PRP were incubated with 10 % CaCl2 (final concentration 22.8 mM) at 37 °C for 7 days. At the end of the incubation period, the formulations were spun at 2800g for 15 min, and the supernatants were collected and assayed for growth factors and pro-inflammatory cytokine concentrations using the Quantikine Human Immunoassay kits (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions.
Yin W., Qi X., Zhang Y., Sheng J., Xu Z., Tao S., Xie X., Li X, & Zhang C. (2016). Advantages of pure platelet-rich plasma compared with leukocyte- and platelet-rich plasma in promoting repair of bone defects. Journal of Translational Medicine, 14, 73.
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6

Quantification of Growth Factors in Activated PRP

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ELISA was performed to quantify PDGF-AB and TGF-β1 concentrations in activated PRP samples as described elsewhere [14 (link)]. Briefly, PRP samples were incubated in 10% calcium chloride (final concentration 22.8 mM) for seven days at 37°C in a humidified atmosphere with 5% CO2. Then, the formulations were spun at 2800 g for 15 minutes to collect the supernatant. PDGF-AB and TGF-β1 concentrations in the supernatant were determined using the Quantikine Human Immunoassay kits (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions.
Shen B., Zhang Z., Zhou N.F., Huang Y.F., Bao Y.J., Wu D.S, & Zhang Y.D. (2017). Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 3578-3584.
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7

Serum Adhesion Molecules and Inflammatory Analytes

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Serum adhesion molecules (soluble E-selectin [sE-Selectin], soluble P-selectin [sP-Selectin], soluble intercellular adhesion molecule-1 [sICAM-1], and soluble vascular cell adhesion molecule-1 [sVCAM-1]), as well as inflammatory analytes (interleukin [IL]-6, IL-1β, tumor necrosis factor-α), were measured using a Quantikine human immunoassay kits (R&D Systems, Minneapolis, MN, USA). All these inter-assay CVs ranged between 4 and 8%.
Basu A., Izuora K., Betts N.M., Ebersole J.L, & Scofield R.H. (2021). Dietary Strawberries Improve Biomarkers of Antioxidant Status and Endothelial Function in Adults with Cardiometabolic Risks in a Randomized Controlled Crossover Trial. Antioxidants, 10(11), 1730.
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8

Quantification of Growth Factors and Cytokines in Platelet-Rich Plasma

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The concentrations of PDGF-AB, TGF-β1, IL-1β and TNF-α in the whole blood, L-PRP and P-PRP samples were quantified according to the protocols described previously (14 (link)). In brief, the whole blood, L-PRP and P-PRP samples were incubated with 10% CaCl2 (final concentration, 22.8 mM) at 37°C in a humidified atmosphere containing 5% CO2 for 7 days. At the end of the incubation period, the supernatants were collected, and the concentrations of growth factors and pro-inflammatory cytokines were determined using an enzyme-linked immunosorbent assay (ELISA) with the Quantikine Human Immunoassay kits (R&D Systems, Inc., Minneapolis, MN, USA) according to manufacturer's protocol.
Yin W., Xu H., Sheng J., Xu Z., Xie X, & Zhang C. (2017). Comparative evaluation of the effects of platelet-rich plasma formulations on extracellular matrix formation and the NF-κB signaling pathway in human articular chondrocytes. Molecular Medicine Reports, 15(5), 2940-2948.
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9

Serum Biomarker Profiling in Trastuzumab Therapy

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We collected serum samples at four time points: before treatment (baseline), immediately before the second and fourth trastuzumab administrations, and after confirmation of progressive disease (PD). Serum HER2 levels were measured by a chemiluminescence immunoassay (Siemens Healthcare Diagnostic, Tokyo, Japan). All other serum markers were measured by enzyme-linked immunosorbent assay (ELISA): EGF, TGF-a, and NRG1 levels were measured with DuoSet ELISA Development Systems kits (R&D Systems, Minneapolis, MN, USA), and HGF, IGF1, and tissue inhibitor of metalloproteinase 1 were measured with Quantikine human immunoassay kits (R&D Systems). All assays were performed in duplicate. The lower limits of detection were 3.91 pg/ml for EGF, 7.81 pg/ml for TGF-a, and 62.5 pg/ml for NRG1.
Miura Y., Sukawa Y., Hironaka S., Mori M., Nishikawa K., Tokunaga S., Okuda H., Sakamoto T., Taku K., Nishikawa K., Moriwaki T., Negoro Y., Kimura Y., Uchino K., Shinozaki K., Shinozaki H., Musha N., Yoshiyama H., Tsuda T., Miyata Y., Sugimoto N., Shirakawa T., Ito M., Yonesaka K., Yoshimura K., Boku N., Nosho K., Takano T, & Hyodo I. (2018). Five-weekly S-1 plus cisplatin therapy combined with trastuzumab therapy in HER2-positive gastric cancer: a phase II trial and biomarker study (WJOG7212G). Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 21(1).
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10

Adiponectin Levels in Hemorrhagic Shock

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The human subject study was approved by the University of Texas Health Science Center at Houston (UTHSC-H) Committee for the Protection of Human Subjects as waiver of informed consent. Severely injured patients in hemorrhagic shock with a systolic blood pressure <90 mmHg and/or a base deficit of >5 meq/ml who received at least one unit of blood after Emergency Department (ED) arrival but prior to Intensive Care Unit (ICU) admission were eligible for the study. Subject demographics, laboratory values and outcomes were obtained from patient records. Blood samples were obtained upon admission to the ED (referenced as pre-resuscitation) and subsequently upon arrival to the ICU (referenced as post-resuscitation) at Memorial Hermann Hospital, Houston, TX, a Level I trauma center. Blood samples were also obtained from healthy subjects following obtainment of consent. After collection, blood samples were centrifuged and plasma aliquots prepared and stored at -80°C for subsequent analysis. Levels of total human adiponectin in plasma were measured using Quantikine Human Immunoassay kit from R&D Systems (Minneapolis, MN).
Deng X., Cao Y., Huby M.P., Duan C., Baer L., Peng Z., Kozar R.A., Doursout M.F., Holcomb J.B., Wade C.E, & Ko T.C. (2016). Adiponectin in Fresh Frozen Plasma Contributes to Restoration of Vascular Barrier Function after Hemorrhagic Shock. Shock (Augusta, Ga.), 45(1), 50-54.
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