L 1 gentamicin

Human lung carcinoma cell lines A549 were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The cells were incubated in Kaighn’s modification of F-12 Ham Nutrient Mixture supplemented with 10% fetal bovine serum (FBS) and antibiotics (1% Antibiotic-Antimycotic 100 × and 50 × 10⁻³ g L⁻¹ gentamicin; Biosera) at 37 °C, 95% humidity and 5% CO₂. The cells (10,000/cm⁻²) were seeded on 12-well μ-Chamber slides (ibidi GmbH, Martinsried, Germany) on 6, 12 and 96-well plates (TPP, Trasadingen, Switzerland) and left to settle for 24 h. This incubation method has been published previously [39 (link)].

Human lung carcinoma cell line A549 and CCD-18Co colon fibroblasts were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The A549 cells were cultured in a complete RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) and CCD-18Co cells were cultured in a minimum essential medium (MEM) (PAN-Biotech GmbH, Aidenbach, Germany) at 37 °C, 95% humidity and 5% CO₂. The media were supplemented with 10% fetal bovine serum (FBS, Biosera, Nuaille, France) and antibiotics (1% Antibiotic-Antimycotic 100 × and 50 × 10⁻³ g L⁻¹ gentamicin, Biosera). Prior to the selected treatments, cells were seeded on 6- and/or 96-well plates (TPP, Trasadingen, Switzerland) and left to settle for 24 h. The acridine compounds solutions (at concentrations ranging from 5–75 µM) were then added to cells for 24 or 48 h, and analysis was subsequently performed.