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HMGB2 is a protein that functions as a DNA-binding protein. It is involved in the regulation of transcription and plays a role in the organization of chromatin structure.

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Lab products found in correlation

Pparγ, by Cell Signaling Technology (2 mentions) C ebpα, by Cell Signaling Technology (2 mentions) Gapdh, by Merck Group (2 mentions) Ampkα, by Cell Signaling Technology (2 mentions) Phospho p ampkα, by Cell Signaling Technology (2 mentions) P akt1, by Cell Signaling Technology (2 mentions) L glutamine, by Corning (2 mentions) Penicillin streptomycin, by Corning (2 mentions) Dulbecco s modified eagle s medium dmem high glucose, by Corning (2 mentions) Atcc pcs210010, by American Type Culture Collection (2 mentions) Fibrillarin, by Santa Cruz Biotechnology (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Usp18, by Cell Signaling Technology (1 mentions) Ifit2, by Proteintech (1 mentions) Rig 1, by Proteintech (1 mentions) Isg15, by Proteintech (1 mentions)

3 protocols using hmgb2

1

Adipocyte Differentiation Protocol

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Mouse embryo fibroblast 3T3-L1 (ATCC®CL173) and normal human primary subcutaneous preadipocytes (ATCC® PCS210010) cells were purchased from American Type Culture Collection (ATCC, Manassas, VA). Dulbecco’s modified Eagle’s medium high glucose (DMEM), penicillin/streptomycin and L-glutamine were purchased from Mediatech, Inc. (Manassas, VA). PPARγ (Cat# 2443S), C/EBPα (Cat# 2295S), FAS (Cat# 4233S), HMGB2 (Cat# 14163S), AMPKα (Cat# 5832S) and phospho(p)-AMPKα (Cat# 50081S-Thr172), Akt1(Cat# 75692S) and p-Akt1 (Cat# 9018S-Ser473) antibodies were from Cell Signaling Technology (Boston, MA). GAPDH (Cat# G9545) antibodies were from Sigma-Aldrich (St. Louis, MO). All secondary antibodies (Cat# 305-035-045) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). All other chemicals were obtained from Sigma-Aldrich unless otherwise stated.
LEUNG I., VEISAGA M.L., ESPINAL M., ZHANG W., BARNUM R, & BARBIERI M.A. (2021). Anti-lipid droplets accumulation effect of Annona montana (mountain soursop) leaves extract on differentiation of preadipocytes. Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 46(3), 567-578.
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2

Immunoblotting Protocol for Protein Analysis

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Cells were lysed, and immunoblotting was performed as previously described (Bianco and Mohr, 2017 (link)). The antibodies used in this study were as follows: TIF-IA (Bethyl Laboratories; A303-065A) UBF (Santa Cruz; SC-13125), Akt (Cell Signaling; 9272), Fibrillarin (Santa Cruz; sc-166001), UL44 (Virusys; CA006), pp28 (Virusys; CA004-100), IE1/IE2 (Millipore; MAB810), HMGB2 (Cell Signaling Technology; 14163), USP18 (Cell Signaling Technology; 4813), IFIT2 (Proteintech; 12604–1-AP), TFIIIA (Bethyl; A303-621A), ISG15 (Proteintech; 15981–1-AP), RIG-I (Proteintech; 20566), MDA5 (Proteintech; 21775–1-AP), UBA7 (Cell Signaling Technology; 69023), actin (Cell Signaling Technology; 3700), BrdU (Sigma; B2531), p53 (Cell Signaling Technology; 9282).
Bianco C, & Mohr I. (2019). Ribosome biogenesis restricts innate immune responses to virus infection and DNA. eLife, 8, e49551.
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3

Adipocyte Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryo fibroblast 3T3-L1 (ATCC®CL173) and normal human primary subcutaneous preadipocytes (ATCC® PCS210010) cells were purchased from American Type Culture Collection (ATCC, Manassas, VA). Dulbecco’s modified Eagle’s medium high glucose (DMEM), penicillin/streptomycin and L-glutamine were purchased from Mediatech, Inc. (Manassas, VA). PPARγ (Cat# 2443S), C/EBPα (Cat# 2295S), FAS (Cat# 4233S), HMGB2 (Cat# 14163S), AMPKα (Cat# 5832S) and phospho(p)-AMPKα (Cat# 50081S-Thr172), Akt1(Cat# 75692S) and p-Akt1 (Cat# 9018S-Ser473) antibodies were from Cell Signaling Technology (Boston, MA). GAPDH (Cat# G9545) antibodies were from Sigma-Aldrich (St. Louis, MO). All secondary antibodies (Cat# 305-035-045) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). All other chemicals were obtained from Sigma-Aldrich unless otherwise stated.
LEUNG I., VEISAGA M.L., ESPINAL M., ZHANG W., BARNUM R, & BARBIERI M.A. (2021). Anti-lipid droplets accumulation effect of Annona montana (mountain soursop) leaves extract on differentiation of preadipocytes. Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 46(3), 567-578.
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