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Af 214 14

Manufactured by Thermo Fisher Scientific
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Sourced in China

The AF-214-14 is a laboratory equipment product made by Thermo Fisher Scientific. It is designed to perform a specific core function, but a detailed description cannot be provided while maintaining an unbiased and purely factual approach. More information would be required to describe the product accurately without interpretation or extrapolation.

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Lab products found in correlation

Cerulenin, by Merck Group (2 mentions) Etomoxir, by Merck Group (2 mentions) Allopurinol, by Merck Group (2 mentions) Oleic acid, by Cayman Chemical (2 mentions) Simvastatin, by Merck Group (2 mentions) Mk 2206, by Cayman Chemical (2 mentions) 25 hydroxycholesterol, by Cayman Chemical (2 mentions) Diphenyleneiodonium chloride dpi, by Merck Group (2 mentions) L ng nitroarginine methyl ester l name, by Cayman Chemical (2 mentions) Sirt1 activator 2, by Merck Group (2 mentions) Ex 527, by Merck Group (2 mentions) Aicar, by Abcam (2 mentions) Hmgcoa, by Merck Group (2 mentions) Cholesterol methyl β cyclodextrin, by Merck Group (2 mentions) D4902, by Merck Group (2 mentions) Compound c, by Merck Group (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Anti mouse il 4 antibody, by Thermo Fisher Scientific (2 mentions) Af 315 02, by Thermo Fisher Scientific (1 mentions)

5 protocols using af 214 14

1

Polarization of Bone Marrow-Derived Macrophages

Cited 1 time
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BMMФ were stimulated either with IL-4 (10 ng/ml, AF-214-14, Peprotech), lipopolysaccharide (100 ng/ml, Sigma) or dexamethasone (100nM, D4902, Sigma) to respectively achieve M(IL-4), M(LPS) or M(GC) polarization state. In some experiments, BMMФ were treated with C75 (Sigma), Cerulenin (Sigma), Etomoxir (Sigma), pan-AKT inhibitor MK-2206 (1 mM, Cayman Chemical), 25-hydroxycholesterol (10 μM, Cayman chemical), N-acetyl cysteine (10mM, Sigma), Diphenyleneiodonium chloride (DPI, 5 μM, Sigma), L-NG-Nitroarginine methyl ester (L-NAME, 1mM, Cayman Chemical), Allopurinol (100 μM, Sigma), hydrogen peroxide (Sigma), SIRT1 activator II (Sigma), EX-527 (Sigma), Compound C (Sigma), AICAR (Abcam), HMGCoA (Sigma), water-soluble cholesterol (Cholesterol–methyl-β-cyclodextrin, Sigma) or Simvastatin (Sigma).
For fatty acid treatment, palmitic or oleic acid (Cayman) were solubilized at 100mM in absolute ethanol at 60°C. Fatty acid were conjugated at the desired concentration using a sonicator water bath into the macrophage culture medium to avoid endotoxin contamination from BSA54 (link).
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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2

Bone Marrow-Derived Macrophage Isolation and Polarization

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To derive bone marrow-derived macrophages (BMDMs), bone marrow cells freshly isolated from the femora of Prep-/- and Prep+/- littermates were seeded in 6-well plates, and cultivated in RPMI 1640 supplemented with 10% FBS (Gibco, 16000-044), a penicillin/streptomycin mix (Gibco, 15140122) and 50 ng/ml M-CSF (PeproTech, AF-315-02) at 37 °C with 5% CO2. BMDMs were fully differentiated and ready for use on Day 7. For macrophage polarization experiments, BMDMs were left unstimulated, stimulated with 50 ng/ml LPS, or stimulated with 20 ng/ml murine Interleukin-4 (PeproTech, AF-214-14) and 20 ng/ml murine Interleukin-13 (PeproTech, AF-315-02) for 24 h. RAW 264.7 cells were obtained from the China Center for Type Culture Collection (Shanghai, China) and cultured and maintained in DMEM supplemented with 10% FBS at 37 °C with 5% CO2. NIH-3T3 cells were purchased from Procell Life Science Technology Co., Ltd. (Wuhan, China) and were cultured and maintained in DMEM supplemented with 10% CS (AusGeneX, NCS-S) at 37 °C with 5% CO2.
Lin S.Z., Wu W.J., Cheng Y.Q., Zhang J.B., Jiang D.X., Ren T.Y., Ding W.J., Liu M., Chen Y.W, & Fan J.G. (2023). Prolyl endopeptidase remodels macrophage function as a novel transcriptional coregulator and inhibits fibrosis. Experimental & Molecular Medicine, 55(7), 1437-1450.
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3

Murine IL-4 Immune Modulation

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Animal-free recombinant murine IL-4 (AF-214-14, Peprotech) was suspended at a concentration of 500 μg/ml and mixed with anti-mouse IL-4 antibody (BioXcell clone 11b11) at a molar ratio of 2:1 (weight 1:5) for 1–2 minutes at room temperature to complex IL-4 with the antibody. The complex (IL-4c) was suspended in PBS to a concentration of 25μg/ml IL-4 and 125 μg/ml of 11b11. Each mouse was injected intraperitoneally with 200μl of IL-4c (5 μg IL-4 and 25 μg 11b11) on day 0 and peritoneal exudate cells (PECs) were collected on day 2.
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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4

Polarization of Bone Marrow-Derived Macrophages

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
BMMФ were stimulated either with IL-4 (10 ng/ml, AF-214-14, Peprotech), lipopolysaccharide (100 ng/ml, Sigma) or dexamethasone (100nM, D4902, Sigma) to respectively achieve M(IL-4), M(LPS) or M(GC) polarization state. In some experiments, BMMФ were treated with C75 (Sigma), Cerulenin (Sigma), Etomoxir (Sigma), pan-AKT inhibitor MK-2206 (1 mM, Cayman Chemical), 25-hydroxycholesterol (10 μM, Cayman chemical), N-acetyl cysteine (10mM, Sigma), Diphenyleneiodonium chloride (DPI, 5 μM, Sigma), L-NG-Nitroarginine methyl ester (L-NAME, 1mM, Cayman Chemical), Allopurinol (100 μM, Sigma), hydrogen peroxide (Sigma), SIRT1 activator II (Sigma), EX-527 (Sigma), Compound C (Sigma), AICAR (Abcam), HMGCoA (Sigma), water-soluble cholesterol (Cholesterol–methyl-β-cyclodextrin, Sigma) or Simvastatin (Sigma).
For fatty acid treatment, palmitic or oleic acid (Cayman) were solubilized at 100mM in absolute ethanol at 60°C. Fatty acid were conjugated at the desired concentration using a sonicator water bath into the macrophage culture medium to avoid endotoxin contamination from BSA54 (link).
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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5

Murine IL-4 Immune Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal-free recombinant murine IL-4 (AF-214-14, Peprotech) was suspended at a concentration of 500 μg/ml and mixed with anti-mouse IL-4 antibody (BioXcell clone 11b11) at a molar ratio of 2:1 (weight 1:5) for 1–2 minutes at room temperature to complex IL-4 with the antibody. The complex (IL-4c) was suspended in PBS to a concentration of 25μg/ml IL-4 and 125 μg/ml of 11b11. Each mouse was injected intraperitoneally with 200μl of IL-4c (5 μg IL-4 and 25 μg 11b11) on day 0 and peritoneal exudate cells (PECs) were collected on day 2.
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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