Bcl xl sc 56021

MHC specific antibody was covalently conjugated to N-hydroxysuccinamide magnetic beads (Pierce) per manufacturer’s protocol. 50 to 100 ug protein equivalent of EVs were incubated with antibody beads overnight at 4°C. The bead bound EV fractions were separated per manufacturer’s protocol. EVs bound to beads were eluted using tris glycine and utilized for downstream analysis. Unconjugated HLA allele-specific anti-HLA A2 monoclonal IgG antibody (Catalogue # 0791HA) was purchased from One Lambda (West Hills, CA, USA), for donor HLA class I specific exosome isolation from recipient mouse plasma total pool of exosomes. Antibodies to insulin (15848–1-AP; used at a dilution of 1:200), TSG 101(28283–1-AP; used at a dilution of 1:500) were purchased from Proteintech Lab; antibodies to GLP1R (sc-390774; used at a dilution of 1:200), GLP-1(sc-57166; used at a dilution of 1:200), Bcl-2 (sc-7382; used at a dilution of 1:200), and Bcl-XL (sc-56021; used at a dilution of 1:200) were purchased from Santa Cruz Biotechnologies, Inc. Secondary antibodies conjugated to HRP (ready-to-use anti-rabbit, anti-mouse were purchased from Vector Lab: MP7451 and MP7452, respectively).