Dmem medium

Manufactured by GE Healthcare

Sourced in United States, Germany, Austria, China, Australia

DMEM medium is a cell culture medium commonly used to support the growth and maintenance of a variety of cell types in laboratory settings. It provides the necessary nutrients, salts, and other components required for cell proliferation and survival.

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Lab products found in correlation

Fetal bovine serum (fbs), by GE Healthcare (4 mentions) Rpmi 1640 medium, by GE Healthcare (3 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (3 mentions) Penicillin, by PanEco (3 mentions) Streptomycin, by PanEco (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Fetal calf serum (fcs), by GE Healthcare (2 mentions) Glutamine, by PanEco (2 mentions) Nahco3, by PanEco (2 mentions) Hydrocortisone hc, by Merck Group (2 mentions) Dmem f12 medium, by Biosera (2 mentions) Rpm1 1640 medium, by Biosera (2 mentions) Egf epithelial growth factor, by Merck Group (2 mentions) Penicillin streptomycin, by GE Healthcare (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Mcf 7, by GE Healthcare (2 mentions) Zeocin, by InvivoGen (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Matrigel, by GE Healthcare (2 mentions) 1640 medium, by GE Healthcare (2 mentions)

Spelling variants of this product

DMEM (338 citations) Dulbecco's modified Eagle's medium (DMEM) (44 citations) HyClone™ Dulbecco’s modified Eagle’s medium (DMEM, (9 citations) Hyclone™ Dulbecco’s Modified Eagle Medium (DMEM) (4 citations) High glucose Dulbecco's modified Eagle's medium (DMEM) (4 citations) DMEM/high glucose Hyclone medium (3 citations) Dulbecco's modified Eagle's medium (DMEM)/F12 (3 citations) Hyclone DMEM medium (3 citations) DMEM/F12 medium (HyClone (2 citations) Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium (2 citations)

32 protocols using dmem medium

Prostate Cell Line Cultivation and Maintenance

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Two human PCa cell lines (DU145 and LNCaP) and one benign prostate cell line (RWPE-1) were purchased from American Type Culture Collection (Manassas, VA, USA). DU145 and LNCaP cell lines were maintained in the 1640 Medium (HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum, and RWPE-1 cells were cultured in DMEM Medium (HyClone; GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum. The cells were cultured at 37°C in a humidified incubator with 5% CO₂.

Huang Y., Jiang G., Liang X., Lan Z., Su Z., Wu H., Weng J, & Jiang X. (2018). Elevated expression of PTCD3 correlates with tumor progression and predicts poor prognosis in patients with prostate cancer. Molecular Medicine Reports, 18(4), 3914-3922.

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Murine Colon Adenocarcinoma Cell Models

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The murine colon adenocarcinoma cell lines, MC38 and CT26, was purchased from the American Type Culture Collection (Rockville, MD, USA). MC38 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium (GE Healthcare, USA) supplemented with 10% fetal bovine serum (FBS, VWR, USA). CT26 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (GE Healthcare, USA) supplemented with 10% FBS. Medium was further supplemented with 100 U per mL penicillin G sodium and 100 μg per mL streptomycin sulfate. Cells were cultured in a humidified atmosphere containing 5% CO₂ at 37 °C. C57Bl/6 and Balb/c mice (6–8 weeks) were obtained from Harlan-Envigo Laboratories, Inc (USA). Mycoplasma was tested before use by MycoAlert detection kit (Lonza Nottingham, Ltd.) The study protocol was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Chicago.

Ni K., Lan G., Veroneau S.S., Duan X., Song Y, & Lin W. (2018). Nanoscale metal-organic frameworks for mitochondria-targeted radiotherapy-radiodynamic therapy. Nature Communications, 9, 4321.

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APN Peptide Neuroprotection in HT22 Cells

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Mouse HT22 hippocampal cells were cultured in DMEM medium (GE Healthcare Life Sciences, Logan, UT, USA) containing 10% (v/v) fetal calf serum (FBS) and 0.35% glucose and supplemented with duplex antibiotics of 100 U/mL penicillin and 100 μg/mL streptomycin, in a humidified atmosphere of 5% CO₂ and 95% air at 37°C. The APN peptide was dissolved in dimethyl sulfoxide (DMSO) and diluted with culture medium immediately prior to use. DMSO (0.01%) was used as a sham control. The cells were pretreated with different concentrations of the APN peptide for 6 h before being exposed to Glu. The concentrations and durations of Glu exposure in the Glu group were selected based on a previous study [30 (link)]. After relevant treatments, cells were collected for further analysis.

Yue L., Zhao L., Liu H., Li X., Wang B., Guo H., Gao L., Feng D, & Qu Y. (2016). Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1α Expression in HT22 Hippocampal Neurons. Oxidative Medicine and Cellular Longevity, 2016, 2957354.

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Anti-inflammatory Signaling Pathway Assay

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DMEM medium and Fetal calf serum were obtained from GE Healthcare; anti-TNF-α antibody (sc-1350) and anti-NF-κB antibody (sc-372) were obtained from Santa Cruz Biotechnology; anti-IRS-1Ser307 antibody (AI623) was obtained from Beyotime Biotechnology, China, and anti-GAPDH (TA-08) and ECL display color liquid were purchased from Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., China.

Zhang H., Ta N., Chen P, & Wang H. (2017). Erchen Decoction and Linguizhugan Decoction Ameliorate Hepatic Insulin Resistance by Inhibiting IRS-1Ser307 Phosphorylation In Vivo and In Vitro. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 1589871.

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Cell Culture of TLR4 and NOD2 Reporters

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RAW-Blue, HEK-Blue-hTLR4, HEK-Blue-hNOD2, and control HEK-Blue-Null2 cells were obtained from Invivogen (San Diego, CA, USA) and maintained in Dulbecco‘s Modified Eagle’s medium (DMEM) medium (GE Healthcare, Chicago, IL, USA) supplemented with 10% fetal calf serum (Thermo Scientific, Waltham, MA, USA), 50 U/mL penicillin, 50 μg/mL streptomycin, 2 mM glutamine, and 0.1 M NaHCO₃ (all PanEco, Moscow, Russia) at 37 °C with 5% CO₂.

Tukhvatulin A., Dzharullaeva A., Erokhova A., Zemskaya A., Balyasin M., Ozharovskaia T., Zubkova O., Shevlyagina N., Zhukhovitsky V., Fedyakina I., Pruss I., Shcheblyakov D., Naroditsky B., Logunov D, & Gintsburg A. (2020). Adjuvantation of an Influenza Hemagglutinin Antigen with TLR4 and NOD2 Agonists Encapsulated in Poly(D,L-Lactide-Co-Glycolide) Nanoparticles Enhances Immunogenicity and Protection against Lethal Influenza Virus Infection in Mice. Vaccines, 8(3), 519.

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Sumac Extract's Effects on Breast Cancer Cells

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In the in vitro experiments, human breast cancer cell lines MCF-7 (ER+, PR+, HER2−), MDA-MB-231 (ER− PR−, HER2−) and non-cancer MCF-10A (human mammary gland epithelial cells) were used. Breast cancer cells were cultured in DMEM medium (GE Healthcare, Piscataway, NJ, USA) or RPM1 1640 medium (Biosera, Kansas City, MO, USA) and non-cancer cells were cultured in DMEM F12 medium (Biosera, Kansas City, MO, USA) + supplemented with insulin, EGF- epithelial growth factor, HC-hydrocortisone (all Sigma, Steinheim, Germany). The growth medium was supplemented with 10% FBS (Gibco), antibiotic/antimycotic solution (1× HyClone™; GE Healthcare, Chicago, IL, USA) and cells were cultivated in an atmosphere containing 5% CO₂ in humidified air at 37 °C. Before each experiments, the cell viability was estimated by trypan blue exclusion (≥95%).
For the flow cytometry experiments, the MCF-7 (3 × 10⁵) and MDA-MB-231 (1 × 10⁵) cells were seeded in Petri dishes and cultivated for 24 h in a complete cultivation medium. The sumac extract (SONNENTOR Kräuterhandels GMBH, Sprögnitz, Austria) was added to every experimental group for 24, 48, and 72 h prior to analysis.

Kubatka P., Kello M., Kajo K., Samec M., Liskova A., Jasek K., Koklesova L., Kuruc T., Adamkov M., Smejkal K., Svajdlenka E., Solar P., Pec M., Büsselberg D., Sadlonova V, & Mojzis J. (2020). Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma. International Journal of Molecular Sciences, 22(1), 183.

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Culturing Mouse Bladder Cancer Cells

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The mouse bladder cancer cell line MB49 was obtained from the Chinese Academy of Sciences. MB49 cells were cultured in DMEM medium (GE Healthcare Life Sciences, Pittsburgh, PA, USA), supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Science, Massachusetts, USA).

Jiang Z., Zhang Y., Zhang Y., Jia Z., Zhang Z, & Yang J. (2021). Cancer derived exosomes induce macrophages immunosuppressive polarization to promote bladder cancer progression. Cell Communication and Signaling : CCS, 19, 93.

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Murine Cancer Cell Line Cultivation

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Murine triple-negative breast cancer cell line 4T1 was kindly provided by Dr. Stephen J. Kron at University of Chicago Murine colon adenocarcinoma cell CT26 and murine squamous cell carcinoma SCC VII were purchased from the American Type Culture Collection (Rockville, MD, USA). CT26 cells was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (GE Healthcare, USA). 4T1 and SCC VII cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium (GE Healthcare, USA). All media were supplemented with 10% FBS, 100 U/mL penicillin G sodium and 100 μg/mL streptomycin sulfate. Cells were cultured in a humidified atmosphere containing 5% CO₂ at 37°C. Mycoplasma was tested before use by MycoAlert detection kit (Lonza Nottingham, Ltd.) BALB/c mice (6 – 8 weeks) and C3H mice (6 – 8 weeks) were obtained from Harlan-Envigo Laboratories, Inc (USA). The study protocol was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Chicago.

Ni K., Lan G., Chan C., Duan X., Guo N., Veroneau S.S., Weichselbaum R.R, & Lin W. (2019). Ultrathin metal-organic layer-mediated radiotherapy-radiodynamic therapy enhances immunotherapy of metastatic cancers. Matter, 1(5), 1331-1353.

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THP-1 Cells Transduced with TMEM126B shRNA

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THP-1 cells (derived from a male donor) were incubated at 37 °C with 5% CO₂ in DMEM medium (GE Healthcare, Munich, Germany) with 10% FCS and 1% penicillin/streptomycin (PAA Laboratories, Cölbe, Germany). THP-1 cells were stably transduced with a lentiviral shRNA (Mission shRNA) against TMEM126B (sh126B: V2LHS_175840) and selected using puromycin. Controls (shC) are THP-1 cells transduced with a pLKO.1-puro vector.

Fuhrmann D.C., Wittig I, & Brüne B. (2018). TMEM126B deficiency reduces mitochondrial SDH oxidation by LPS, attenuating HIF-1α stabilization and IL-1β expression. Redox Biology, 20, 204-216.

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Inducible DDX39B Overexpression in HeLa Cells

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HeLa cells stably expressing an inducible DDX39B cDNA trans-gene were generated using the Flp-In T-Rex system (Life Technologies) as recommended by the manufacturer. HeLa Flp-In T-Rex cell line (Kaiser et al., 2008 (link)) was kindly provided to our group by Dr. E Dobrikova (Duke University). The coding sequence of DDX39B was amplified with Phusion High-Fidelity DNA polymerase (New England BioLabs) using as template cDNA prepared from total RNA isolated from human PBMCs. Forward and reverse primers contained HindIII and XhoI restriction sites, respectively. The resulting PCR amplicon was cloned into pcDNA5/FRT/TO plasmid using HindIII and XhoI restriction sites and verified by Sanger sequencing. This plasmid was co-transfected with pOG44 plasmid, which encodes the Flp recombinase, into HeLa Flp-In T-Rex cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions. Transfected cells were grown in DMEM medium supplemented with 10% FBS free of tetracycline (GE Healthcare Life Sciences) under blasticidin/hygromycinB selection for 15 days, and resistant cells were expanded and used for subsequent experiments. Expression of the trans-gene was induced by addition of tetracycline at 50 μg/ml.

Galarza-Muñoz G., Briggs F.B., Evsyukova I., Schott-Lerner G., Kennedy E.M., Nyanhete T., Wang L., Bergamaschi L., Widen S.G., Tomaras G.D., Ko D.C., Bradrick S.S., Barcellos L.F., Gregory S.G, & Garcia-Blanco M.A. (2017). Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk. Cell, 169(1), 72-84.e13.

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