Analytical grade methanol

Analytical standard of metronidazole (MET) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Two types of titanium(IV) oxide were used in this study—namely Degussa P25 (from Evonik, Essen, Germany) and TiO₂ in the form of anatase from Sigma-Aldrich (St. Louis, MO, USA). Based on information from the manufacturer, the size of the anatase particles was <25 nm, while the specific surface area ranged from 45 to 55 m² g⁻¹. In the case of P25, its particle size was about 27 nm, and the specific surface area was at the level 57 m² g⁻¹. The other semiconductor catalysts such as zinc oxide, tungsten(VI) oxide, zircon (IV) oxide, and lead(II) sulphide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Analytical-grade methanol was purchased from CHEMPUR (Piekary Śląskie, Poland). Hypergrade acetonitrile, formic acid, trifluoroacetic acid, water, and methanol were obtained from Merck (Darmstadt, Germany) and used for the LC analyses.

Analytical standards of sulfamethoxazole (SMX) and trimethoprim (TRI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hypergrade acetonitrile, formic acid, and water were purchased from Merck (Darmstadt, Germany). Analytical-grade methanol, hydrochloric acid, sulfuric acid, and disodium phosphate were purchased from CHEMPUR (Piekary Śląskie, Poland). Analytical-grade acetic acid was purchased from POCH S.A. (Gliwice, Poland). Analytical-grade acetonitrile was purchased from Eurochem (Tarnów, Poland). Analytical-grade citric acid, formic acid, and acetic acid were purchased from ChemLand (Stargard, Poland). Analytical-grade sodium hydroxide was purchased from POCH S.A. (Gliwice, Poland). Oasis HLB cartridges of varying masses of sorbent (500 mg, 6 mL; 400 mg, 6 mL; 200 mg, 3 mL) from Waters (Eschborn, Germany) were used for solid-phase extraction.

The following reagents were used for the mineralization of lyophilized biomass, fruiting bodies, and media: 65% HNO₃ Suprapur^® and 30% H₂O₂ Suprapur^® from Merck (Darmstadt, Germany). Bioelement content standards (Ca, Cu, Fe, K, Mg, Mn, Na, Zn) of 1 g/L concentration were purchased from the District Measurements Office (Łódź, Poland). Artificial digestive juices were prepared using the following: HCl and pancreatic extract from Merck (Darmstadt, Germany); bile salts and pepsin from BTL (Łódź, Poland); CaCl₂ and MgCl₂ from Chempur (Kraków, Poland); NaCl from Pol-Aura (Dywity, Poland); NaHCO₃ from PPH Golpharm (Kraków, Poland); and Na₂HPO₄, KHCO₃, K₂HPO₄, and citric acid from Avantor Performance Materials Poland (Gliwice, Poland). HPLC (high-performance liquid chromatography)-grade standards of cordycepin, lovastatin, ergothioneine, L-phenylalanine, sterols, and indole and phenolic compounds were obtained from Merck (Darmstadt, Germany). Analytical-grade methanol, acetic acid, phosphoric acid, and ammonium acetate were purchased from Chempur (Gliwice, Poland). HPLC-grade methanol, acetonitrile, and KH₂PO₄ were purchased from Merck (Darmstadt, Germany). Water (quadruple-distilled) with a conductivity of less than 1 µS/cm was obtained using an S2-97A2 distillation apparatus from Chemland (Stargard, Poland).