APL was obtained from Shandong Xinhua Pharmaceutical Co., Ltd. (Shandong, China). K. pneumoniae (strain ID: 46114) was purchased from the National Center for Medical Culture Collection (CMCC, Beijing, China). Mayer’s hematoxylin and 1% eosin alcohol solution were purchased from MUTO PURE CHEMICALS (Tokyo, Japan). Thirty-two Sprague–Dawley rats (16 male and 16 female; 200 ± 20 g) were obtained from the Experimental Animal Center of Henan province (Zhengzhou, China). The animals were housed in a temperature (25 ± 2°C) and humidity (50 ± 10%)-controlled environment with a 12:12-h light/dark cycle. Feed and water were available ad libitum. All animals were handled with humane care throughout the experiment.
Eosin alcohol solution
Manufactured by Muto Pure Chemicals
Sourced in Japan
1% eosin alcohol solution is a laboratory reagent used for various purposes in scientific research and analysis. It is a 1% solution of the dye eosin in an alcohol-based solvent. This solution is commonly used in staining and histological procedures to enhance the visibility and contrast of cellular structures under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Mayer s hematoxylin, by Muto Pure Chemicals (6 mentions)
Malinol, by Muto Pure Chemicals (3 mentions)
Formalin neutral buffer solution, by Fujifilm (2 mentions)
Chloral hydrate, by Nacalai Tesque (2 mentions)
Diff quik, by Sysmex (2 mentions)
Porcine pancreatic elastase, by Merck Group (1 mentions)
Methylcellulose, by Fujifilm (1 mentions)
Potassium dihydrogen phosphate, by Fujifilm (1 mentions)
Icr mice, by Charles River Laboratories (1 mentions)
Mepenzolate, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Vascular endothelial growth factor (vegf), by Santa Cruz Biotechnology (1 mentions)
Mmp 9, by Santa Cruz Biotechnology (1 mentions)
Matrix metalloproteinase mmp 2, by Santa Cruz Biotechnology (1 mentions)
Timp 1, by Santa Cruz Biotechnology (1 mentions)
Rneasy kit, by Qiagen (1 mentions)
Cell counting kit, by Dojindo Laboratories (1 mentions)
Hyclone fetal bovine serum fbs, by Thermo Fisher Scientific (1 mentions)
Cellstain double staining kit, by Dojindo Laboratories (1 mentions)
F12 medium, by Thermo Fisher Scientific (1 mentions)
8 protocols using eosin alcohol solution
1
Antibiotic Efficacy Testing in Rats
2
Porcine Pancreatic Elastase and Methacholine Chloride Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Porcine pancreatic elastase (PPE) and methacholine chloride (methacholine) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Novo-Heparin for injection was from Mochida Pharmaceutical, Co. (Tokyo, Japan). Chloral hydrate was from Nacalai Tesque (Kyoto, Japan). Diff-Quik was from Sysmex, Co. (Kobe, Japan). Formalin neutral buffer solution was from WAKO Pure Chemicals (Tokyo, Japan). Mayer’s hematoxylin, 1% eosin alcohol solution and malinol were from MUTO Pure Chemicals (Tokyo, Japan).
3
Analysis of Mepenzolate in Mouse Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mepenzolate, PPE and HPLC-grade acetonitrile were obtained from Sigma-Aldrich (St. Louis, MO). Novo-Heparin for injection was from Mochida Pharmaceutical Co. (Tokyo, Japan). Chloral hydrate was from Nacalai Tesque (Kyoto, Japan). Diff-Quik was from the Sysmex Co (Kobe, Japan). Sodium 1-propanesulfonate was from Tokyo Kasei Chemical Co (Tokyo, Japan). The Amicon utra-0.5 centrifugal filter unit was purchased from Merck Millipore (Billerica, MA). Formalin neutral buffer solution, potassium dihydrogen phosphate and methylcellulose were from WAKO Pure Chemicals (Tokyo, Japan). Mayer's hematoxylin, 1% eosin alcohol solution and malinol were from MUTO Pure Chemicals (Tokyo, Japan). ICR mice (4–6 weeks old, male) were purchased from Charles River (Yokohama, Japan). The experiments and procedures described here were carried out in accordance with the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Institutes of Health, and were approved by the Animal Care Committee of Keio University.
4
Aminophylline and Klebsiella pneumoniae Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aminophylline was obtained from Shandong Xinhua Pharmaceutical Co., Ltd. (Zibo, People’s Republic of China). Klebsiella pneumoniae (strain ID: 46114) was purchased from the National Center for Medical Culture Collection (Beijing, People’s Republic of China). Antibodies against interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α, soluble TNF-α receptor 2, collagen I, collagen III, collagen IV, endothelin (ET)-1, transforming growth factor (TGF)-β, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), matrix metalloproteinase (MMP)-2, MMP-9, and tissue inhibitor of MMP (TIMP)-1 were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The RNeasy kit was obtained from Qiagen (Valencia, CA, USA). Mayer’s hematoxylin and 1% eosin alcohol solution were purchased from MUTO Pure Chemicals (Tokyo, Japan). In all, 42 Sprague Dawley rats (21 male and 21 female; 200±20 g) were purchased from the Experimental Animal Center of Henan Province (Zhengzhou, People’s Republic of China). The animals were housed in cages with free access to food and tap water under standard conditions of humidity (50%±10%), temperature (25°C±2°C), and light (12 hours light/12 hours dark cycle). All animals were handled with humane care throughout the experiment.
5
HPBCD-Assisted Cell Culture Examination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPBCD was kindly donated by Nihon Shokuhin Kako Co., Ltd. (Tokyo, Japan). Mayer's hematoxylin, 1% eosin alcohol solution, and mounting medium for histological examination (malinol) were from MUTO Pure Chemicals (Tokyo, Japan). Dulbecco's modified Eagle's medium and F-12 medium were obtained from Gibco-Life Technologies (Life Technologies Japan, Tokyo, Japan). HyClone™ fetal bovine serum (FBS) was purchased from Thermo Scientific (Logan, UT, USA). The cell counting kit and Cellstain® Double Staining Kit were obtained from Dojindo Laboratories (Kumamoto, Japan). All other reagents and solvents were of reagent grade. De-ionized and distilled bio-pure grade water was used throughout the study.
6
Histological Analysis of Submandibular Glands
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SMGs from the respective mice were fixed with 4% (w/v) paraformaldehyde at 4°C overnight and equilibrated with 30% (w/v) sucrose. The specimens were frozen in OCT compound (Sakura Finetek Japan, Tokyo, Japan) and cut into 10-μm sections. For hematoxylin and eosin (HE) staining, the sections were stained with Mayer's Hematoxylin (Muto pure chemicals, Tokyo, Japan) and 1% Eosin Alcohol Solution (Muto pure chemicals) for 5 min each. For Azan staining, the sections were incubated with 5% (v/v) trichloroacetic acid and 5% (v/v) potassium dichromate for 10 min, Azocarmine G Solution (Wako Pure Chemical Industries, Osaka, Japan) for 30 min, 0.1% (v/v) aniline in 70% (v/v) ethanol for 3 sec, 0.1% (v/v) acetic acid in 95% (v/v) ethanol for 1 min, 5% (w/v) phosphotungstic acid for 60 min, and Aniline blueorange G solution (Wako Pure Chemical Industries) for 3 min. The slides were evaluated under the light microscope (BZ-X710; Keyence Corporation, Osaka, Japan).
7
Histological Analysis of Rat Cornea
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H&E staining of rat cornea cryosections was performed as described previously with minor modifications. Hematoxylin staining was performed with hematoxylin solution (Type M, Muto Pure Chemicals, Tokyo, Japan) for 1 min, and sections were then stained for 30 s with 0.3% eosin alcohol solution (Muto Pure Chemicals). Images of stained rat cornea sections were captured with an Olympus BX53 microscope using a 10 × objective lens and software (Standard Cellsens, Olympus, Tokyo, Japan).
8
Hematoxylin and Eosin Staining of Mouse Retina
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H&E staining of mouse retina cryosections was performed as described previously with minor modifications (Ikuta et al, 2017 (link)). Briefly, hematoxylin staining was performed with hematoxylin solution (Type M; Muto Pure Chemicals) and eosin staining was performed with 0.3% eosin alcohol solution (Muto Pure Chemicals).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!