Drabkin reagent kit 525

Manufactured by Merck Group

Sourced in United States

The Drabkin reagent kit 525 is a laboratory product manufactured by Merck Group. It is a ready-to-use solution designed for the colorimetric determination of hemoglobin. The kit provides the necessary reagents and protocols to perform this analysis.

Automatically generated - may contain errors

Lab products found in correlation

Matrigel matrix, by BD (1 mentions) Heparin, by BD (1 mentions) Rat anti cd31 antibody, by BD (1 mentions) Anti cd31 antibody, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Drabkin reagent (36 citations) Drabkin’s reagent kit 525 (10 citations) Drabkin reagent kit (4 citations) Drabkin kit (2 citations)

13 protocols using drabkin reagent kit 525

In Vivo Angiogenesis Assay with VEGF and Heparin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 6

The matrigel containing VEGF (100 ng/ml) and heparin (24-26 U/ml) is subcutaneously injected into B6 mice. After 5 days the gels are recovered, weighed and processed for hemoglobin quantification or histology as previously described. Hemoglobin content is measured with a Drabkin reagent kit 525 (Sigma). For histological analyses, the matrigel pellets are fixed in 4% paraformaldehyde and embedded in paraffin; four micron sections are stained with hematoxylin-eosin by standard procedures.

An aliquot (300 μl) of MATRIGEL™ (Becton Dickinson Lab.) containing VEGF (150 ng) and heparin (30 IU) was injected subcutaneously into the dorsal region of 6-8 week-old C57BL/6 mice. The MATRIGEL™ formed a plug rapidly. AR-NP (1 mg/kg) or ARLDDL (SEQ ID NO: 351) (1 mg/kg) (see Table 13 for consensus sequence) was administered once intravenously 24 hr later. After 5 days, plugs were taken and photographed (upper panel). Neovessels were quantified by measuring the hemoglobin of the plugs as an indication of blood vessel formation with the Drabkin method and Drabkin reagent kit 525 (Sigma) (B&C). The analysis showed that AR-NP was more effective than ARLDDL (SEQ ID NO: 351) when the drug was injected only once during 5 days angiogenesis period. See FIG. 3.

US10508137B2. Disintegrin variants and pharmaceutical uses thereof (2019-12-17). National Cheng Kung University [TW]. Inventors: Woei-Jer Chuang [TW].

+ Open protocol

+ Expand

Quantifying Functional Vascularization in Matrigel Plugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hemoglobin estimation from the Matrigel was performed by Drabkin’s method [26 ]. To quantify the formation of functional vasculature in the Matrigel plug, the amount of hemoglobin was measured using a Drabkin reagent kit 525 (Sigma, MO) following the Drabkin and Austin methods [27 (link)]. Briefly, the Matrigel plugs were homogenized in a Dounce homogenizer on ice in the presence of 0.5 mL deionized water and allowed to stand overnight at 4 °C. The lysate was centrifuged at 5000×g for 10 min and the supernatant was collected. Next, 0.3 mL of each sample was mixed with 0.5 mL of Drabkin’s reagent and allowed to stand for 15 min at room temperature. The absorbance was read at 540 nm by using Drabkin’s reagent solution as blank. A standard curve was constructed by using known concentrations of hemoglobin, and the concentration of the samples was obtained from the standard curve.

Chaudhary P., Gibbs L.D., Maji S., Lewis C.M., Suzuki S, & Vishwanatha J.K. (2020). Serum exosomal-annexin A2 is associated with African-American triple-negative breast cancer and promotes angiogenesis. Breast Cancer Research : BCR, 22, 11.

+ Open protocol

+ Expand

Matrigel Plug Angiogenesis Assay in Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Matrigel plug angiogenesis assay was performed as described previously [15 (link)]. Briefly, a mixture of BD Matrigel matrix (0.5 mL) and heparin (50 unit/mL; BD Bioscience) was subcutaneously injected into C57BL/6 mice (aged 6–7 weeks) with VEGF (50 ng/mL) and MSSV (125 and 250 µg/mL). After 7 days, mice were euthanized and the Matrigel plugs were removed. Vascularization was determined by measuring the hemoglobin content of the Matrigel plugs using the Drabkin method (Drabkin reagent kit 525, Sigma-Aldrich, Louis, MO, USA) as well as the infiltrated endothelial cell count in the Matrigel plugs via CD31 antibody staining. All animal experiments were performed with the approval of the Animal Care and Use Committee of Chungbuk National University.

Song J.H., Park J., Park S.L., Hwang B., Kim W.J., Lee C, & Moon S.K. (2021). A Novel Cyclic Pentadepsipeptide, N-Methylsansalvamide, Suppresses Angiogenic Responses and Exhibits Antitumor Efficacy against Bladder Cancer. Cancers, 13(2), 191.

+ Open protocol

+ Expand

Matrigel Plug Assay for Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Matrigel plug assay was performed as previously described^{36 (link)}. Briefly, C57BL6 mice (Orient Technology, Seoul, Korea; weighing ~20 g; 7 weeks of age) were injected subcutaneously with 0.6 mL Matrigel containing saline, ECG supplement (354006; BD Bioscience), ECG + 4-HBA (0.1 mM), ECG + PDGF-BB (20 nM), or ECG plus 4-HBA (0.1 mM) and PDGF-BB (20 nM). After 7 days, the mice were anesthetized using Zoletil (30 mg/kg) and Rompun (10 mg/kg) by intraperitoneal injection. Next, the skins of the mice were pulled back to expose the Matrigel plug, which remained intact. The animals were then euthanized by carbon dioxide inhalation. Haemoglobin obtained from the Matrigel was measured using a Drabkin reagent kit 525 (Sigma-Aldrich) in order to qualify blood vessel formation. The concentration of haemoglobin was calculated in comparison to a known amount of haemoglobin assayed in parallel.

Kang C.W., Han Y.E., Kim J., Oh J.H., Cho Y.H, & Lee E.J. (2017). 4-Hydroxybenzaldehyde accelerates acute wound healing through activation of focal adhesion signalling in keratinocytes. Scientific Reports, 7, 14192.

+ Open protocol

+ Expand

Xenograft tumor angiogenesis model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nude mice were manipulated and cared for according to NIH Animal Care and Use Committee guidelines in the Experiment Animal Center of the Nanjing Medical University (Nanjing, China). The protocol was approved by the Committee on the Ethics of Animal Experiments of the Nanjing Medical University. Female athymic BALB/c nu/nu mice, 3–4 weeks old (Model Animal Research Center of Nanjing University, Nanjing, China), were maintained under pathogen‐free conditions. Cell aliquots (100 μL) were mixed with an equal volume of high concentration matrigel, and then injected subcutaneously into the right flanks of the nude mice. The gel plugs were excised from nude mice 7 days after inoculation. The hemoglobin content of the matrigel was determined using a Drabkin reagent kit 525 (Sigma). Histological sections on slides were stained with hematoxylin and eosin (HE), and with monoclonal antibodies recognizing endothelial cell marker CD31 and CD34 and the vascular/lymphatic marker VEGF.

Qu S., Wu J., Bao Q., Yao B., Duan R., Chen X., Li L., Yuan H., Jin Y, & Ma C. (2018). Osterix promotes the migration and angiogenesis of breast cancer by upregulation of S100A4 expression. Journal of Cellular and Molecular Medicine, 23(2), 1116-1127.

+ Open protocol

+ Expand

Matrigel Plug Angiogenesis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 mice (7 weeks of age) were given s.c. injections of 600 µL of Matrigel (Collaborative Biomedical Products, Bedford, MA) at 4℃, containing the indicated amount (25 µg) of ATME, 100 ng VEGF, and 10 units heparin. After injection, the Matrigel rapidly formed a plug. After 7 days, the skin of the mouse was pulled back to expose the Matrigel plug, which remained intact. After quantitative differences were noted and imaged, hemoglobin was measured using the Drabkin method and Drabkin reagent kit 525 (Sigma) to quantify blood vessel formation. The hemoglobin concentration was calculated using a known amount of hemoglobin assayed in parallel.

Kim E.C., Kim S.H., Piao S.J., Kim T.J., Bae K., Kim H.S., Hong S.S., Lee B.I, & Nam M. (2015). Antiangiogenic Activity of Acer tegmentosum Maxim Water Extract in Vitro and in Vivo. Journal of Korean Medical Science, 30(7), 979-987.

+ Open protocol

+ Expand

Measuring Tumor Angiogenesis via Hemoglobin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumours from each animal were removed, and haemoglobin was measured as an indication of blood vessel formation using the Drabkin method (Drabkin reagent kit 525; Sigma-Aldrich) 34 (link). The concentration of haemoglobin was calculated from a known amount of haemoglobin assayed in parallel.

Lai K.C., Hsu S.C., Yang J.S., Yu C.C., Lein J.C, & Chung J.G. (2014). Diallyl trisulfide inhibits migration, invasion and angiogenesis of human colon cancer HT-29 cells and umbilical vein endothelial cells, and suppresses murine xenograft tumour growth. Journal of Cellular and Molecular Medicine, 19(2), 474-484.

+ Open protocol

+ Expand

Measuring Angiogenesis in Matrigel Plugs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

2-month-old C57BL/6 mice were injected subcutaneously with 0.5 ml of Matrigel containing heparin (10 U/ml), IL-5, VEGF (50 ng/mL) or IL-5 antibody (2 μg/ml). After 7 days, the matrigel plugs were removed and photographed. The quantification of vascularization was determined by measuring the hemoglobin content using the Drabkin method (Drabkin reagent kit 525, Sigma-Aldrich, Louis, MO). The infiltrating endothelial cells were identified by immunochemistry using a CD-31 antibody. Confocal microscopy was performed using previously described antibody-conjugated QD565 nanoparticles7 (link)8 (link).

Park S.L., Chung T.W., Kim S., Hwang B., Kim J.M., Lee H.M., Cha H.J., Seo Y., Choe S.Y., Ha K.T., Kim G., Yun S.J., Park S.S., Choi Y.H., Kim B.K., Kim W.T., Cha E.J., Patterson C., Kim W.J, & Moon S.K. (2017). HSP70-1 is required for interleukin-5-induced angiogenic responses through eNOS pathway. Scientific Reports, 7, 44687.

+ Open protocol

+ Expand

Matrigel Plug Assay for Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Angiogenesis was evaluated in 5–6-wk-old male C57BL/6 mice using the Matrigel plug assay as previously described (Choi et al., 2009 (link)). 500 µl of growth factor–reduced Matrigel containing 200 ng VEGF and 10 U heparin was mixed with the indicated amount of either PTD-A2 or 25-µM control peptide. After injection, the Matrigel rapidly formed a single, solid gel plug. After 5 d, the skin of the mouse was pulled back to expose the Matrigel plug, which remained intact. To quantify blood vessel formation, hemoglobin was measured using the Drabkin method and the Drabkin reagent kit 525 (Sigma-Aldrich). The concentration of hemoglobin was calculated by comparison to a known amount of hemoglobin assayed in parallel. To identify infiltrating ECs, immunohistochemistry was performed using rat anti-CD31 antibody (BD).

Park H., Lee S., Shrestha P., Kim J., Park J.A., Ko Y., Ban Y.H., Park D.Y., Ha S.J., Koh G.Y., Hong V.S., Mochizuki N., Kim Y.M., Lee W, & Kwon Y.G. (2015). AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation. The Journal of Cell Biology, 211(3), 619-637.

+ Open protocol

+ Expand

Angiogenic Potential of LysRS-DKK2-Fc

Check if the same lab product or an alternative is used in the 5 most similar protocols

An aliquot of 600 μL liquid Matrigel at 4 °C was mixed with 14 nM LysRS-DKK2-Fc or TEV-cleaved LysRS-DKK2-Fc and injected into the abdominal subcutaneous tissues of seven-week-old male C57BL/6 mice. The injected Matrigel rapidly formed a solid gel plug. After three days, the mouse skin was pulled back to expose the Matrigel plug and quantify blood vessel infiltration. Hb levels were measured using the Drabkin Reagent Kit 525 (Sigma-Aldrich) [13 (link)]. Animal experiment was approved by the institutional animal care and use committee (IACUC) of Yonsei University (IACUC-A-201801-158-01).

Lee H.M., Kwon S.B., Son A., Kim D.H., Kim K.H., Lim J., Kwon Y.G., Kang J.S., Lee B.K., Byun Y.H, & Seong B.L. (2019). Stabilization of Intrinsically Disordered DKK2 Protein by Fusion to RNA-Binding Domain. International Journal of Molecular Sciences, 20(11), 2847.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!