Anti cbp

Manufactured by Merck Group

Sourced in Switzerland

Anti-CBP is a laboratory reagent used for the detection and quantification of the CREB-binding protein (CBP) in biological samples. It functions as an antibody that specifically binds to CBP, enabling researchers to analyze its expression and activity in various experimental settings.

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Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions) Anti pgk1, by Abcam (1 mentions) Odyssey equipment, by LI COR (1 mentions) Image studio lite ver 5, by LI COR (1 mentions) Anti gfp, by Merck Group (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Sc 9996, by Santa Cruz Biotechnology (1 mentions) Protein a g magnetic beads, by Merck Group (1 mentions) Anti gr, by Santa Cruz Biotechnology (1 mentions) Anti smrt, by Merck Group (1 mentions) Anti pparγ, by Merck Group (1 mentions) Amersham protran 0.45 m nc, by GE Healthcare (1 mentions) Anti gfp, by Roche (1 mentions)

Spelling variants of this product

Anti-CBP antibody (5 citations) Anti-CBP tag antibody (2 citations) Anti-p300/CBP Ab (1 citations)

4 protocols using anti cbp

Immunoblot Analysis of Yeast Complexes

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Yeast whole-cell lysates, the fractions from the glycerol gradient sedimentation, and the coimmunoprecipitated proteins were resuspended in SDS sample buffer, resolved on 8 or 10% SDS-PAGE (Tris-glycine running buffer), transferred to nitrocellulose membranes (Ambion) and incubated with anti-CBP (Millipore), anti-GFP (Sigma-Aldrich), anti-uL18 (gift from Dr Cleslei F. Zanelli, UNESP), anti-Nog1 (gift from Dr John L. Woolford Jr., Carnegie Mellon University) and anti-Pgk1 (Abcam). Secondary antibodies conjugated to IR700dye (anti-rabbit IgG, LI-COR) or IR800dye (anti-mouse IgG, LI-COR) were employed, and near-infrared Western blot detection was carried out using ChemiDoc MP Imaging System (BioRad) or Odyssey equipment (LI-COR). Images were processed using Image Studio™ Lite (ver. 5.2) software (LI-COR).

Bagatelli F.F., de Luna Vitorino F.N., da Cunha J.P, & Oliveira C.C. (2021). The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events. Nucleic Acids Research, 49(12), 7053-7074.

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Visualizing Protein Complexes in Yeast

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Cell extracts from strain MJM01 (Cdc5-GFP, Lea1-protein A, Snu114-CBP), MJM02 (Brr2-GFP, Lea1-protein A, Snu114-CBP), and MJM03 (Spp42-GFP, Lea1-protein A, Snu114-CBP) were used for Western blots. Total proteins were separated by 4%–12% SDS-PAGE and transferred to PVDF membrane. Western blots were carried out by using anti-GFP (SC-9996, Santa Cruz) with 800RD anti-rabbit IgG, anti-CBP (07-482, Millipore) with 680RD anti-mouse IgG, and detected on an Odyssey scanner (fluorescence).

Chen W., Shulha H.P., Ashar-Patel A., Yan J., Green K.M., Query C.C., Rhind N., Weng Z, & Moore M.J. (2014). Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes. RNA, 20(3), 308-320.

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Epididymal Fat Protein Interaction

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Protein isolated from epididymal fat pads was incubated overnight with protein A/G magnetic beads (Millipore) and an Anti-SMRT (Millipore #17-10057) or Anti-GR (Santa Cruz #sc-1004) antibody. In the morning the beads were washed with RIPA buffer three times, then denatured in Laemmle buffer at 95°C. The resulting proteins were run on a western blot and probed with Anti-GR (Santa Cruz #sc-1004), Anti-CBP (#sc-369), Anti-Med1 (#sc-5334), Anti-PPARγ (#sc-7273), or Anti-SMRT (Millipore #17-10057) antibodies. Blots were performed at least three times.

Emont M.P., Mantis S., Kahn J.H., Landeche M., Han X., Sargis R.M, & Cohen R.N. (2015). Silencing Mediator of Retinoid and Thyroid Hormone Receptors (SMRT) regulates glucocorticoid action in adipocytes. Molecular and cellular endocrinology, 407, 52-56.

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Protein Detection by Western Blotting

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Proteins were separated by SDS or Native PAGE and transferred to either a PVDF (Immobilon FL PVDF 0.45 µm, Millipore, Billerica, MA) or a nitrocellulose (Amersham Protran 0.45 µm NC, GE healthcare, Chicago, IL) membranes using standard methods. Antibodies used were anti-GFP (Roche, Basel, Switzerland) and anti-CBP (Millipore). Signals were visualized using an Odyssey IR scanner (LI-CORE Biosciences, Lincoln, NE).

Berglund L.L., Hao X., Liu B., Grantham J, & Nyström T. (2017). Differential effects of soluble and aggregating polyQ proteins on cytotoxicity and type-1 myosin-dependent endocytosis in yeast. Scientific Reports, 7, 11328.

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